Zobrazeno 1 - 10
of 20
pro vyhledávání: '"J M, Vanderkooi"'
Publikováno v:
Journal of Fluorescence. 13:59-67
Fluorescence line narrowing is a sensitive tool to monitor subtle changes in protein conformation. We restudied the consequences of inhomogeneous broadening on the observed spectra. Vibronic absorption bands of systems with weak electron-phonon coupl
Publikováno v:
European journal of biochemistry. 268(4)
We have recently demonstrated that metalloporphyrins are potent inhibitors of both human immunodeficiency virus type 1 (HIV-1) and human immunodeficiency virus type 2 (HIV-2) reverse transcriptases (RTs) [Argyris, E.G., Vanderkooi, J.M., Venkateswara
Publikováno v:
Biospectroscopy. 5(3)
Charged groups reside mainly on protein surfaces, but for proteins that incorporate redox centers, a charge typically exists at the prosthetic group within the interior. How a protein accommodates a buried charge and the effect of redox changes on pr
Publikováno v:
Methods in enzymology. 278
Perhaps the most important contribution of FLN is that it provides an experimental approach to relate physical changes in the protein to predicted dynamical behavior. It is clear that the sample is inhomogeneously broadened in a continuous manner, co
Publikováno v:
The Journal of biological chemistry. 269(37)
The fluorescence properties of porphyrin were compared in protoporphyrin-protoheme hybrid hemoglobins (Hb) in which the protohemes (Fe) in either the alpha or beta subunits were substituted with protoporphyrin IX, alpha (P)2 beta (Fe)2-Hb and alpha (
Publikováno v:
Biochemistry. 31(28)
The influence of the protein matrix on the reactivity of external molecules with a species buried within the protein interior is considered in two general ways: (1) there may be structural fluctuations that allow for the diffusive penetration of the
Publikováno v:
Proceedings in Life Sciences ISBN: 9781461291596
Optical spectra of biological molecules are structure less under normal measuring conditions. However, using site selection conditions, i.e., narrow laser band excitation and low temperatures (4.2K), resolved spectra are obtained which allow for opti
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::35b21f164770d8cf448ebc72defa27ba
https://doi.org/10.1007/978-1-4612-4796-8_43
https://doi.org/10.1007/978-1-4612-4796-8_43
Publikováno v:
The Journal of biological chemistry. 262(12)
An optical method for measuring oxygen concentrations in aqueous solutions is described. This method is based upon the oxygen-dependent quenching of phosphorescence. Phosphorescence excitation and emission spectra and lifetimes of some of the probe m
Autor:
P.-I. Ohlsson, T. Horie, J. M. Vanderkooi, K.-G. Paul, Povl Krogsgaard-Larsen, Ragnar Ryhage, Roland Isaksson
Publikováno v:
Acta chemica Scandinavica. Series B: Organic chemistry and biochemistry. 40(4)
Fluorescent derivatives of horseradish peroxidase C were prepared by replacing protoheme by protoporphyrin or mesoporphyrin. Calculations according to Forster on energy transfer allowed the determination of the distances of greater than 2.2 nm betwee
Autor:
J M, Vanderkooi, D F, Wilson
Publikováno v:
Advances in experimental medicine and biology. 200
The oxygen dependent quenching of phosphorescence, if measured by the oxygen dependence of the phosphorescence lifetime, can be used as a sensitive and precise measure of oxygen concentration. The result is an optical method for measuring oxygen whic