Zobrazeno 1 - 10
of 20
pro vyhledávání: '"J M, Mancheño"'
Autor:
Javier Lacadena, Mercedes Oñaderra, A Martínez del Pozo, José Manuel Pérez-Cañadillas, Marta Bruix, J M Mancheño, José G. Gavilanes, Antonio Martínez-Ruiz, C. De Antonio
Publikováno v:
Proteins: Structure, Function, and Genetics. 41:350-361
α-Sarcin, a potent cytotoxic protein from Aspergillus giganteus, contains two tryptophan residues at positions 4 and 51. Two single, W4F and W51F, and the double mutant, W4/51F, have been produced and purified to homogeneity. These two residues are
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::908b3f0704580ef6865b416eb02f72d3
https://doi.org/10.1002/1097-0134(20001115)41:3<350::aid-prot70>3.0.co
https://doi.org/10.1002/1097-0134(20001115)41:3<350::aid-prot70>3.0.co
Autor:
Álvaro Martínez del Pozo, José G. Gavilanes, Mercedes Oñaderra, J M Mancheño, Javier Lacadena, María Gasset
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology. 1252:43-52
alpha-Sarcin is a ribosome-inactivating protein that translocates across lipid bilayers, these two abilities explaining its cytotoxic character. This protein is composed of a single polypeptide chain with two disulfide bridges. Reduction and carboxya
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fc39d86c4c28de431b15154789a0d7a2
https://doi.org/10.1016/0167-4838(95)00106-5
https://doi.org/10.1016/0167-4838(95)00106-5
Autor:
Mercedes Oñaderra, Javier Lacadena, María Gasset, Fernando Ramón, J M Mancheño, José G. Gavilanes, A Martínez del Pozo
Publikováno v:
Biophysical Journal. 67(3):1117-1125
alpha-Sarcin is a fungal cytotoxic protein that inactivates the eukaryotic ribosomes. A kinetic study of the aggregation and lipid mixing promoted by this protein on phosphatidylglycerol (PG) and phosphatidylserine (PS) vesicles has been performed. E
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::41ac0b4486fe072aa5c8e0d412eae62d
Autor:
Javier Lacadena, Mercedes Oñaderra, J M Mancheño, Carlos López-Otín, María Gasset, José G. Gavilanes, A Martínez del Pozo, Juan Antonio García, J L Barbero, S Ortega
Publikováno v:
Gene. 142:147-151
An efficient system was developed to produce, in Escherichia coli, large amounts of native alpha-sarcin (alpha Sar), a cytotoxin from the mold Aspergillus giganteus. The protein has been purified to homogeneity with a yield of 1.5 micrograms/ml of or
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2f8c87ac4e49a60c5a0d6c3f0d9506de
https://doi.org/10.1016/0378-1119(94)90370-0
https://doi.org/10.1016/0378-1119(94)90370-0
Autor:
Giampietro Schiavo, José G. Gavilanes, Javier Lacadena, María Gasset, Mercedes Oñaderra, A Martínez del Pozo, J M Mancheño
Publikováno v:
Scopus-Elsevier
ResearcherID
Europe PubMed Central
ResearcherID
Europe PubMed Central
alpha-Sarcin is a cytotoxic protein produced by the mould Aspergillus giganteus. Insertion of alpha-sarcin into asolectin membranes has been demonstrated by protein labelling with photoreactive phospholipids. alpha-Sarcin added externally to tRNA-con
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3c0e1103c6541513a261538f50a2d122
https://doi.org/10.1042/bj2950221
https://doi.org/10.1042/bj2950221
Publikováno v:
Biochemical Journal. 291:907-914
Penicillin acylase (PA) from Kluyvera citrophila was inhibited by N-ethoxycarbonyl-2-ethoxy-1,2-dihydroquinoline (EEDQ), a specific carboxy-group-reactive reagent. Enzyme activity progressively decreased to a residual value depending on EEDQ concentr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::434bb58280e3b155776465adb3c13c31
https://doi.org/10.1042/bj2910907
https://doi.org/10.1042/bj2910907
Autor:
M, Masip, J, Lacadena, J M, Mancheño, M, Oñaderra, A, Martínez-Ruiz, A, Martínez del Pozo, J G, Gavilanes
Publikováno v:
European journal of biochemistry. 268(23)
Alpha-sarcin, a cyclizing ribonuclease secreted by the mould Aspergillus giganteus, is one of the best characterized members of a family of fungal ribotoxins. This protein induces apoptosis in tumour cells due to its highly specific activity on ribos
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::af12d53f081932feba288823a096f5b8
https://pubmed.ncbi.nlm.nih.gov/11733014
https://pubmed.ncbi.nlm.nih.gov/11733014
Autor:
A, Martínez-Ruiz, L, García-Ortega, R, Kao, J, Lacadena, M, Oñaderra, J M, Mancheño, J, Davies, A, Martínez del Pozo, J G, Gavilanes
Publikováno v:
Methods in enzymology. 341
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::e44ce40b40565841c1a1d3e3d42af758
https://pubmed.ncbi.nlm.nih.gov/11582789
https://pubmed.ncbi.nlm.nih.gov/11582789
Autor:
C, de Antonio, A, Martínez del Pozo, J M, Mancheño, M, Oñaderra, J, Lacadena, A, Martínez-Ruiz, J M, Pérez-Cañadillas, M, Bruix, J G, Gavilanes
Publikováno v:
Proteins. 41(3)
alpha-Sarcin, a potent cytotoxic protein from Aspergillus giganteus, contains two tryptophan residues at positions 4 and 51. Two single, W4F and W51F, and the double mutant, W4/51F, have been produced and purified to homogeneity. These two residues a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::5806780ed2fe393fe7cd60eebf6f2bb2
https://pubmed.ncbi.nlm.nih.gov/11025546
https://pubmed.ncbi.nlm.nih.gov/11025546
Autor:
J, Lacadena, A, Martínez del Pozo, A, Martínez-Ruiz, J M, Pérez-Cañadillas, M, Bruix, J M, Mancheño, M, Oñaderra, J G, Gavilanes
Publikováno v:
Proteins. 37(3)
alpha-Sarcin is a ribotoxin secreted by the mold Aspergillus giganteus that degrades the ribosomal RNA by acting as a cyclizing ribonuclease. Three residues potentially involved in the mechanism of catalysis--histidine-50, glutamic acid-96, and histi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::dc8d32daafa5c22fe1775b6fcea5099d
https://pubmed.ncbi.nlm.nih.gov/10591106
https://pubmed.ncbi.nlm.nih.gov/10591106