Zobrazeno 1 - 10
of 18
pro vyhledávání: '"J M, Dellacha"'
Autor:
J M Dellacha, G N Ciccia-Torres
Publikováno v:
Biochemical Journal. 228:761-764
Specific binding of 125I-labelled human somatotropin was demonstrated in isolated hepatocytes from male mice. In the presence of divalent cations (Ca2+ and Mg2+) the binding of 125I-labelled human somatotropin was competitive with ovine prolactin. Sc
Publikováno v:
Journal of Histochemistry & Cytochemistry. 9:271-277
Several Armour thyrotrophin preparations were labeled with different amounts of a fluorescent dye, acid rhodamine B (Lissamine-Rhodamine B 200, Imperial Chemical Industries, Ltd.) following Chadwick's method with slight modifications. The native thyr
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Protein Structure. 168:95-105
1. 1.|The variation of some physicochemical and biological properties of bovine growth hormone dissolved in various acidic buffers, has been examined. 2. 2.|The hormone preparation used was not contaminated with acid proteases. 3. 3.|Measurements of
Publikováno v:
Journal of Histochemistry & Cytochemistry. 10:194-203
Adult albino rats were injected intravenously with labelled homologous albumin, globulins and fibrinogen. Labelling was done with acid rhodamine B (C.I. No. 45100) and fluorescein isothiocyanate. The amount of fluorescent dye bound to proteins was me
Publikováno v:
Acta physiologica et pharmacologica latinoamericana : organo de la Asociacion Latinoamericana de Ciencias Fisiologicas y de la Asociacion Latinoamericana de Farmacologia. 34(2)
The presence of insulin binding sites in a clonal line of cells from rat pituitary tumor (GH1 cells) is described. The binding of insulin was a reversible and specific process. Binding of 125I-insulin to GH1 cells was inhibited by unlabeled insulin a
Publikováno v:
Archivos de biologia y medicina experimentales. 21(1)
Isolated cells from rat submaxillary gland bound 125I-labelled insulin in a time-dependent process that reached a maximum at 30-40 min at 25 degrees C. The radioactivity bound to cells could be dissociated by dilution of the binding site-hormone comp
Publikováno v:
Acta physiologica latino americana. 26(5)
Specific binding of 125I-HGH and 125I-bGH was obtained in hypophysectomized rat liver cells. Two types of somatogenic receptors were found with apparent dissociation constants of 4.2 x 10(8) M-1 and 1.7 x 10(7) M-1. The number of sites for the higher
Publikováno v:
Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas. 17(1)
Insulin labeled with 125I was injected into turtles to study its specific uptake by the thyroid gland. The gel filtration behavior of labeled material in blood and thyroid gland was determined in order to ascertain if the uptake is specific. Most ani
Publikováno v:
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society. 9
Rat serum proteins were labelled in vitro with a fluorescent dye, acid rhodamine B, C.I. No. 45100, using Chadwick's method. Labeled serum was injected intravenously in albino rats weighing 150-200 gm, in doses of 0.8 cc/100 gm body weight. Biochemic
Publikováno v:
Revista de la Sociedad Argentina de Biologia. 37