Zobrazeno 1 - 10 of 10 pro vyhledávání: '"J M, Beechem"'
1
Time-Resolved Fluorescence Anisotropy Study of the Refolding Reaction of the α-Subunit of Tryptophan Synthase Reveals Nonmonotonic Behavior of the Rotational Correlation Time
Autor: Matthews Cr, Jill A. Zitzewitz, J. M. Beechem, Osman Bilsel, Li Yang
Publikováno v: Biochemistry. 38:4177-4187
Time-resolved fluorescence anisotropy of a bound extrinsic probe was studied in an effort to characterize dynamic properties of the transient partially folded forms that appear during the folding of the alpha-subunit of tryptophan synthase (alphaTS)
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ec8fce7172792d83fed893d759404a08
https://doi.org/10.1021/bi9829433
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2
Comparison of three different fluorescent visualization strategies for detecting Escherichia coli ATP synthase subunits after sodium dodecyl sulfate-polyacrylamide gel electrophoresis
Autor: K N, Berggren, E, Chernokalskaya, M F, Lopez, J M, Beechem, W F, Patton
Publikováno v: Proteomics. 1(1)
The correlation between protein molecular weight and the number of lysine or basic amino acid residues was found to be high for broad range molecular weight standards, subunits of Escherichia coli F1F0-ATP synthase and the translated open reading fra
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::a26a846505b2b6390e0318014f9b76a1
https://pubmed.ncbi.nlm.nih.gov/11680898
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3
Molecular mechanism and energetics of clamp assembly in Escherichia coli. The role of ATP hydrolysis when gamma complex loads beta on DNA
Autor: J G, Bertram, L B, Bloom, M M, Hingorani, J M, Beechem, M, O'Donnell, M F, Goodman
Publikováno v: The Journal of biological chemistry. 275(37)
Escherichia coli DNA polymerase III holoenzyme is a multisubunit composite containing the beta sliding clamp and clamp loading gamma complex. The gamma complex requires ATP to load beta onto DNA. A two-color fluorescence spectroscopic approach was ut
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::6ef539fd134d45141c96fd72fe923bc1
https://pubmed.ncbi.nlm.nih.gov/10874049
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4
A model for Escherichia coli DNA polymerase III holoenzyme assembly at primer/template ends. DNA triggers a change in binding specificity of the gamma complex clamp loader
Autor: B, Ason, J G, Bertram, M M, Hingorani, J M, Beechem, M, O'Donnell, M F, Goodman, L B, Bloom
Publikováno v: The Journal of biological chemistry. 275(4)
The gamma complex of the Escherichia coli DNA polymerase III holoenzyme assembles the beta sliding clamp onto DNA in an ATP hydrolysis-driven reaction. Interactions between gamma complex and primer/template DNA are investigated using fluorescence dep
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::88afd1d1bf5068749ad4438524d3bf2f
https://pubmed.ncbi.nlm.nih.gov/10644772
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8
Effects of lateral diffusion on the fluorescence anisotropy in hexagonal lipid phases. II. An experimental study
Autor: Sun-Yung Chen, Kwan Hon Cheng, J. M. Beechem, W van der Meer
Publikováno v: Biophysical Journal. (6):1527-1537
The polymorphic phase behavior of unsaturated phosphatidylethanolamine (PE)/diacylglycerol (DG) binary lipid mixtures was investigated by the use of time-resolved fluorescence anisotropy. Using a fluorescent lipid, 1-palmitoyl-2-[[2-[4-(6-phenyl-tran
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7006c2ab573b9abc5faf7110b3826fe7
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9
Time-resolved fluorescence and 1H NMR studies of tyrosine and tyrosine analogues: correlation of NMR-determined rotamer populations and fluorescence kinetics
Autor: W R, Laws, J B, Ross, H R, Wyssbrod, J M, Beechem, L, Brand, J C, Sutherland
Publikováno v: Biochemistry. 25(3)
The time-resolved fluorescence properties of phenol and straight-chained phenol derivatives and tyrosine and simple tyrosine derivatives are reported for the pH range below neutrality. Phenol and straight-chained phenol derivatives exhibit single exp
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::6f81cc20b42415f3bc71c9a22d0bef5a
https://pubmed.ncbi.nlm.nih.gov/3955016
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10
Simultaneous analysis of multiple fluorescence decay curves by Laplace transforms. Deconvolution with reference or excitation profiles
Autor: M, Ameloot, J M, Beechem, L, Brand
Publikováno v: Biophysical chemistry. 23(3-4)
The properties and potentials of the noniterative Laplace deconvolution (LAP2) (M. Ameloot and H. Hendrickx, Biophys. J. 44 (1983) 27) are further investigated. It is shown that LAP2 is exact and that no extrapolations have to be calculated or assume
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::87209e272314c38ff6b1eadeb7948eff
https://pubmed.ncbi.nlm.nih.gov/3708094
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