Zobrazeno 1 - 10
of 14
pro vyhledávání: '"J L Creelan"'
Publikováno v:
Avian Pathology. 31:493-499
Amplification of avian paramyxovirus serotype 1 (APMV-1)-specific nucleic acid fragments, followed by restriction endonuclease analysis (REA) using BglI, was carried out to type strains according to their virulence. Primer sequences were used to ampl
Publikováno v:
Archives of Virology. 144:1961-1975
The Cux-1 isolate of chicken anaemia virus (CAV), which had received 310 (P310) cell culture passages, was substantially less pathogenic than virus that had been passaged 13 times (P13). Molecularly cloned virus isolates, selected from the P310 and P
Publikováno v:
Avian Pathology. 27:74-79
The Cux-1 isolate of chicken anaemia virus (CAV) was passaged over 320 times in Marek's disease virus transformed chicken lymphoblastoid (MDCC-MSB1) cells. Comparison of the infectivity titres of virus pools derived from viruses that had received 0 (
Publikováno v:
Archives of Virology. 141:1523-1534
Chicken anaemia virus (CAV) is an icosahedral virus, 25 nm in diameter, which, on the basis of its circular single-stranded DNA genome, has recently been classified in the family, Circoviridae. We have investigated whether infectious, monomeric CAV D
Publikováno v:
Avian Pathology. 24:171-187
The pathogenicity of the Cux-1 isolate of chicken anaemia virus (CAV) was substantially reduced following large numbers (50 to 173) of passages in MDCC-MSBl cells. Restriction endonuclease analysis of polymerase chain reaction (PCR)- amplified DNAs a
Publikováno v:
Journal of General Virology. 71:819-823
Chicken anaemia agent (CAA) was purified using differential centrifugation and successive cycles of equilibrium density gradient centrifugation using sucrose and CsCl. The purification method was dependent on the use of an antigen-detecting ELISA bas
Autor:
Neris W. Ball, Gerard F. Mckenna, Daniel Todd, J L Creelan, A. N. J. Scott, Brian M. Meehan, Thomas J. Connor, M S McNulty
Publikováno v:
Avian pathology : journal of the W.V.P.A. 32(4)
An attenuated chicken anaemia virus (CAV) isolate, cloned isolate 10, which was molecularly cloned from the Cuxhaven-1 CAV after 173 cell-culture passages, was shown previously to recover pathogenicity following 10 passages in young chicks. The conse
Autor:
J L, Creelan, S J, McCullough
Publikováno v:
Developments in biologicals. 126
A real-time fluorescent RT-PCR assay was developed to amplify avian paramyxovirus serotype 1 (APMV-1)-specific nucleic acid fragments from field samples. Subsequent restriction endonuclease analysis (REA) using BglI was carried out to type strains ac
Autor:
Samuel J. McCullough, J L Creelan
Publikováno v:
FEMS microbiology letters. 190(1)
Strain-specific primer sequences derived from the helicase gene of an ovine abortifacient strain (S26/3) of Chlamydophila abortus (Chlamydia psittaci) were evaluated for the diagnosis of enzootic abortion in ewes (EAE) by polymerase chain reaction (P
Publikováno v:
FEMS microbiology letters. 171(1)
Enzootic abortion in ewes (EAE) is caused by strains of Chlamydia psittaci which have the ability to invade and colonise the placenta of sheep. In an attempt to improve diagnostic methods for the detection of EAE, subtraction hybridisation was used t