Zobrazeno 1 - 10
of 223
pro vyhledávání: '"J Keith Joung"'
Autor:
Weiting Zhang, Karl Petri, Junyan Ma, Hyunho Lee, Chia-Lun Tsai, J Keith Joung, Jing-Ruey Joanna Yeh
Publikováno v:
eLife, Vol 12 (2024)
CRISPR prime editing (PE) requires a Cas9 nickase-reverse transcriptase fusion protein (known as PE2) and a prime editing guide RNA (pegRNA), an extended version of a standard guide RNA (gRNA) that both specifies the intended target genomic sequence
Externí odkaz:
https://doaj.org/article/ad99b0e410504a6dbdf94fc1701017e7
Autor:
Thomas J Sproule, Robert Y Wilpan, Benjamin E Low, Kathleen A Silva, Deepak Reyon, J Keith Joung, Michael V Wiles, Derry C Roopenian, John P Sundberg
Publikováno v:
PLoS ONE, Vol 18, Iss 10, p e0292456 (2023)
Previous work strongly implicated Collagen 17a1 (Col17a1) as a potent genetic modifier of junctional epidermolysis bullosa (JEB) caused by a hypomorphic mutation (Lamc2jeb) in mice. The importance of the noncollagenous domain (NC4) of COLXVII was sug
Externí odkaz:
https://doaj.org/article/678e25f859e64d80a1b1744863c9b5d2
Autor:
Andrea Schmidts, Leah C Marsh, Ambike A Srivastava, Amanda A Bouffard, Angela C Boroughs, Rebecca C Larson, Felipe Bedoya, Bryan D Choi, Stefanie R Bailey, Mark B Leick, Sonika Vatsa, Michael C Kann, Michelle S Prew, Benjamin P Kleinstiver, J Keith Joung
Publikováno v:
Journal for ImmunoTherapy of Cancer, Vol 8, Iss 2 (2020)
Background Adoptive cell therapy with chimeric antigen receptor T cells (CAR-T) has become a standard treatment for patients with certain aggressive B cell malignancies and holds promise to improve the care of patients suffering from numerous other c
Externí odkaz:
https://doaj.org/article/1383b25b9a8e4f49a0640c17e9556951
Autor:
Katherine W Rogers, Nathan D Lord, James A Gagnon, Andrea Pauli, Steven Zimmerman, Deniz C Aksel, Deepak Reyon, Shengdar Q Tsai, J Keith Joung, Alexander F Schier
Publikováno v:
eLife, Vol 6 (2017)
Developmental signaling pathways often activate their own inhibitors. Such inhibitory feedback has been suggested to restrict the spatial and temporal extent of signaling or mitigate signaling fluctuations, but these models are difficult to rigorousl
Externí odkaz:
https://doaj.org/article/7977447a73d64502b0c629bd2f36edd0
Autor:
Shamim H Rahman, Johannes Kuehle, Christian Reimann, Tafadzwa Mlambo, Jamal Alzubi, Morgan L Maeder, Heimo Riedel, Paul Fisch, Tobias Cantz, Cornelia Rudolph, Claudio Mussolino, J Keith Joung, Axel Schambach, Toni Cathomen
Publikováno v:
PLoS Genetics, Vol 11, Iss 5, p e1005239 (2015)
In vitro disease modeling based on induced pluripotent stem cells (iPSCs) provides a powerful system to study cellular pathophysiology, especially in combination with targeted genome editing and protocols to differentiate iPSCs into affected cell typ
Externí odkaz:
https://doaj.org/article/1408d426c90e4ba8a6c9198e7e74e645
Autor:
Woong Y Hwang, Yanfang Fu, Deepak Reyon, Morgan L Maeder, Prakriti Kaini, Jeffry D Sander, J Keith Joung, Randall T Peterson, Jing-Ruey Joanna Yeh
Publikováno v:
PLoS ONE, Vol 8, Iss 7, p e68708 (2013)
We have previously reported a simple and customizable CRISPR (clustered regularly interspaced short palindromic repeats) RNA-guided Cas9 nuclease (RGN) system that can be used to efficiently and robustly introduce somatic indel mutations in endogenou
Externí odkaz:
https://doaj.org/article/ac7dbb56bb564ab4a308baa536e6c2a3
Autor:
Finola E Moore, Deepak Reyon, Jeffry D Sander, Sarah A Martinez, Jessica S Blackburn, Cyd Khayter, Cherie L Ramirez, J Keith Joung, David M Langenau
Publikováno v:
PLoS ONE, Vol 7, Iss 5, p e37877 (2012)
Zinc Finger Nucleases (ZFNs) made by Context-Dependent Assembly (CoDA) and Transcription Activator-Like Effector Nucleases (TALENs) provide robust and user-friendly technologies for efficiently inactivating genes in zebrafish. These designer nuclease
Externí odkaz:
https://doaj.org/article/06be4b11a7854f728ad26d2a7669fc64
Autor:
Mario Hermann, Morgan L Maeder, Kyle Rector, Joseph Ruiz, Burkhard Becher, Kurt Bürki, Cyd Khayter, Adriano Aguzzi, J Keith Joung, Thorsten Buch, Pawel Pelczar
Publikováno v:
PLoS ONE, Vol 7, Iss 9, p e41796 (2012)
Zinc finger nucleases (ZFNs) enable precise genome modification in a variety of organisms and cell types. Commercial ZFNs were reported to enhance gene targeting directly in mouse zygotes, whereas similar approaches using publicly available resources
Externí odkaz:
https://doaj.org/article/8101fc014e4343dd976109cee43490d9
Autor:
Jeongeun Lee, Andrew S Hirsh, Ben S Wittner, Morgan L Maeder, Rajasekhar Singavarapu, Magdalena Lang, Sailajah Janarthanan, Ultan McDermott, Vijay Yajnik, Sridhar Ramaswamy, J Keith Joung, Dennis C Sgroi
Publikováno v:
PLoS ONE, Vol 6, Iss 7, p e21112 (2011)
Combinatorial libraries of artificial zinc-finger transcription factors (ZF-TFs) provide a robust tool for inducing and understanding various functional components of the cancer phenotype. Herein, we utilized combinatorial ZF-TF library technology to
Externí odkaz:
https://doaj.org/article/94aa3d71087b4f05a83d837f9ac128ed
Autor:
Jonathan E Foley, Jing-Ruey J Yeh, Morgan L Maeder, Deepak Reyon, Jeffry D Sander, Randall T Peterson, J Keith Joung
Publikováno v:
PLoS ONE, Vol 4, Iss 2, p e4348 (2009)
Customized zinc finger nucleases (ZFNs) form the basis of a broadly applicable tool for highly efficient genome modification. ZFNs are artificial restriction endonucleases consisting of a non-specific nuclease domain fused to a zinc finger array whic
Externí odkaz:
https://doaj.org/article/b23ff9aaf9be4861b7f58cdfa38c5f62