Zobrazeno 1 - 10
of 28
pro vyhledávání: '"J K, Northup"'
Publikováno v:
The Biochemical journal. 358(Pt 2)
We have used surface plasmon resonance (SPR) measurements for the kinetic analysis of G-protein-receptor interaction monitored in real time. Functionally active rhodopsin was immobilized on an SPR surface, with full retention of biochemical specific
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::2b829ef9232196cdaef414fe75f7334e
https://pubmed.ncbi.nlm.nih.gov/11513737
https://pubmed.ncbi.nlm.nih.gov/11513737
Publikováno v:
The Journal of biological chemistry. 267(25)
The expression of nucleoside diphosphate kinase (NDK) genes has been implicated as a negative regulator of murine and human tumor metastases and is critical to proper development in Drosophila melanogaster. Molecular mechanisms for the role(s) of NDK
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::d54a1fc39bfcfd58af1065d4a7647fd3
https://pubmed.ncbi.nlm.nih.gov/1325460
https://pubmed.ncbi.nlm.nih.gov/1325460
Publikováno v:
Molecular pharmacology. 40(2)
Angiotensin II can inhibit hormone-stimulated adenylyl cyclase in intact hepatocytes or in hepatic membrane preparations. Because the response can be blocked by pertussis toxin, the object of the present study was to determine which of the known vari
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::60986dbc801d4c3a4ffc357ac76567bf
https://pubmed.ncbi.nlm.nih.gov/1908548
https://pubmed.ncbi.nlm.nih.gov/1908548
Publikováno v:
The Journal of biological chemistry. 266(19)
The beta gamma subunits of G-proteins are composed of closely related beta 35 and beta 36 subunits tightly associated with diverse 6-10 kDa gamma subunits. We have developed a reconstitution assay using rhodopsin-catalyzed guanosine 5'-3-O-(thio)trip
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::7a7c7c52779704cf7ca5a94f70843866
https://pubmed.ncbi.nlm.nih.gov/1905716
https://pubmed.ncbi.nlm.nih.gov/1905716
Publikováno v:
The Journal of biological chemistry. 265(16)
The 23.5-kDa Sec4 protein is required for vesicular transport between the Golgi apparatus and the plasma membrane in Saccharomyces cerevisiae. In order to analyze its biochemical properties, we have purified the soluble pool of the wild-type protein
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::62dc9bdf1215e8b72f6ce5734ffea220
https://pubmed.ncbi.nlm.nih.gov/2111819
https://pubmed.ncbi.nlm.nih.gov/2111819
Publikováno v:
The Journal of biological chemistry. 264(7)
The effects of pertussis toxin on the steady-state levels of G-protein alpha- and beta-subunits were investigated both in vitro and in vivo. The steady-state level Go alpha, a major substrate for pertussis toxin-catalyzed ADP-ribosylation, was unalte
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::e76d80349f024982c8f6fd5057d0e289
https://pubmed.ncbi.nlm.nih.gov/2492997
https://pubmed.ncbi.nlm.nih.gov/2492997
Publikováno v:
The Journal of biological chemistry. 261(15)
Using minor modifications of procedures developed to purify GTP-binding proteins (G-proteins) from rabbit liver, we have purified the major G-proteins present in human placental membranes. One, referred to as Gi, is the major substrate for pertussis
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::3f0fc22fbcab9f2e36d3208717421cd4
https://pubmed.ncbi.nlm.nih.gov/3084494
https://pubmed.ncbi.nlm.nih.gov/3084494
Publikováno v:
The Journal of biological chemistry. 258(18)
Activation of the stimulatory guanine nucleotide-binding regulatory component (G/F) of adenylate cyclase by guanine nucleotides or by Al3+, Mg2+, and F-stabilizes the protein to thermal denaturation or to inactivation by LiBr, guanidine HCl, or urea.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::e594ece16f67be8f2c29120ffea986ad
https://pubmed.ncbi.nlm.nih.gov/6309844
https://pubmed.ncbi.nlm.nih.gov/6309844
Publikováno v:
The Journal of biological chemistry. 263(11)
The wide range of functions attributed to GTP-binding regulatory proteins (G proteins) is reflected in the structural diversity which exists among the alpha, beta, and gamma subunits of G proteins. Recently two cDNA clones encoding beta subunits, bet
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::9f3d4df4292e95735331a6c3eb135052
https://pubmed.ncbi.nlm.nih.gov/3128533
https://pubmed.ncbi.nlm.nih.gov/3128533
Publikováno v:
The Journal of biological chemistry. 257(1)
The requirements for cholera toxin-catalyzed ADP ribosylation of the purified regulatory component of adenylate cyclase are described. In addition to the toxin, this reaction is dependent on or is facilitated by NAD, GTP, phospholipid, and a factor f
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::649f1c663a37b22bddc38b0e46eaffc4
https://pubmed.ncbi.nlm.nih.gov/6273425
https://pubmed.ncbi.nlm.nih.gov/6273425