Zobrazeno 1 - 10
of 63
pro vyhledávání: '"J J Parrish"'
Publikováno v:
Journal of Animal Science. 70:263-272
The proportion of transferable beef embryos obtained after superovulation, follicle aspiration, and in vitro maturation and fertilization has been small. To seek possible explanations, cows on different planes of nutrition were treated with exogenous
Publikováno v:
Biology of reproduction. 60(6)
To test the hypothesis that bull antisperm antibodies have the capacity to interfere with fertilization, antisperm antibodies were generated in three 13-mo-old Holstein bulls by auto-immunizing each bull with sperm three times. All bulls produced ser
Autor:
C M, Medeiros, J J, Parrish
Publikováno v:
Molecular reproduction and development. 44(4)
Changes in the plasma membrane of bovine sperm during heparin-induced capcitation were detected by the binding of fluorescent labeled lectins to unfixed sperm. Of the seven lectins evaluated, only binding of wheat germ agglutinin (WGA) changed with c
Autor:
A I, Mahmoud, J J, Parrish
Publikováno v:
Molecular reproduction and development. 43(4)
Eight different lectins conjugated to fluorescein isothiocyanate (FITC) were used to screen for sperm plasma membrane changes during in vitro capacitation of bovine sperm. Analysis of lectin binding to sperm was done using flow cytometry. Of the eigh
Publikováno v:
Biology of reproduction. 51(6)
The objective of this study was to determine whether the initiation and length of the zygotic S-phase differs for embryos sired by bulls demonstrating high versus low fertility in vivo. Bovine oocytes were matured in vitro for 24-27 h and fertilized
Publikováno v:
Biology of reproduction. 51(5)
The objective of this study was to investigate the importance of cAMP during capacitation of bovine sperm. The competitive antagonist of cAMP, Rp-adenosine-3'5'-cyclic monophosphorothioate (Rp-cAMP), blocked heparin-induced capacitation (p0.05). The
Publikováno v:
Reproduction, Fertility and Development. 20:192
Fourier harmonic analysis (FHA) of sperm nuclei is a precise and objective method to evaluate shape of the sperm head, with the calculated harmonic amplitudes highly related to male fertility. The FHA approach has been developed for use in the bull a
Publikováno v:
Reproduction, Fertility & Development; Jul2010, Vol. 22 Issue 7, p1092-1099, 8p
Publikováno v:
Biology of Reproduction. 35:413-424
Incubation of bull sperm with liposomes made with phosphatidylcholine (PC) containing fatty acyl chains of either 10 (PC10) or 12 (PC12) carbons resulted in greater than 90% of the sperm exhibiting an acrosome reaction (AR) within 15 min. Liposomes o
Publikováno v:
Gamete Research. 10:165-175
The effect of equipment design, separatory media, and time and temperature of separation were studied. Discontinuous 4%/10% bovine serum albumin (BSA) gradients were used to isolate highly motile spermatozoa in rabbit and bull semen. For all conditio