Zobrazeno 1 - 10
of 16
pro vyhledávání: '"J J, Guinovart"'
Autor:
J L, Albarracín, J M, Fernández-Novell, J, Ballester, M C, Rauch, A, Quintero-Moreno, A, Peña, T, Mogas, T, Rigau, A, Yañez, J J, Guinovart, J C, Slebe, I I, Concha, J E, Rodríguez-Gil
Publikováno v:
Biology of reproduction. 71(5)
In vitro capacitation of dog spermatozoa in a medium without sugars and with lactate as the metabolic substrate (l-CCM) was accompanied by a progressive increase of intracellular glycogen during the first 2 h of incubation, which was followed by a su
Autor:
T, Rigau, M, Rivera, M J, Palomo, J M, Fernández-Novell, T, Mogas, J, Ballester, A, Peña, P J, Otaegui, J J, Guinovart, J E, Rodríguez-Gil
Publikováno v:
Reproduction (Cambridge, England). 123(4)
Incubation of dog spermatozoa with 10 mmol l(-1) glucose or fructose rapidly increased the intracellular content of glucose 6-phosphate and fructose 6-phosphate, although the effect of fructose was greater. These effects were correlated with increase
Autor:
M, García-Rocha, A, Roca, N, De La Iglesia, O, Baba, J M, Fernández-Novell, J C, Ferrer, J J, Guinovart
Publikováno v:
The Biochemical journal. 357(Pt 1)
Changes in the intracellular distribution of liver glycogen synthase (GS) might constitute a new regulatory mechanism for the activity of this enzyme at cellular level. Our previous studies indicated that incubation of isolated hepatocytes with gluco
Publikováno v:
The Journal of biological chemistry. 276(26)
We used metabolic control analysis to determine the flux control coefficient of phosphorylase on glycogen synthesis in hepatocytes by titration with a specific phosphorylase inhibitor (CP-91149) or by expression of muscle phosphorylase using recombin
Publikováno v:
The Biochemical journal.
We have used recombinant adenoviruses (AdCMV-RLGS and AdCMV-GK) to overexpress the liver isoforms of glycogen synthase (GS) and glucokinase (GK) in primary cultured rat hepatocytes. Glucose activated overexpressed GS in a dose-dependent manner and ca
Autor:
J, Ballester, J M, Fernández-Novell, J, Rutllant, M, García-Rocha, M, Jesús Palomo, T, Mogas, A, Peña, T, Rigau, J J, Guinovart, J E, Rodríguez-Gil
Publikováno v:
Molecular reproduction and development. 56(2)
The glycogen content in fresh raw dog spermatozoa was 0.22+/-0.03 micromol/mg protein. This matched with the presence of a glycogen-like staining in the head and midpiece. Glycogen levels lowered to 0.05 micromol/mg protein after incubation for 60 mi
Publikováno v:
The Journal of biological chemistry. 265(5)
The mechanism for glycogen synthesis stimulation produced by adenosine, fructose, and glutamine has been investigated. We have analyzed the relationship between adenine nucleotides and glycogen metabolism rate-limiting enzymes upon hepatocyte incubat
Autor:
J. M. Fernández-Novell, J. Ballester, J. Altirriba, L. Ramió-Lluch, A. Barberà, R. Gomis, J. J. Guinovart, J. E. Rodríguez-Gil
Publikováno v:
Reproduction, Fertility & Development; Mar2011, Vol. 23 Issue 3, p468-480, 13p
Autor:
J J, Guinovart, M, Rosell-Pérez
Publikováno v:
Revista espanola de fisiologia. 31(4)
The application of the o-toluidine procedure for glucose estimation was studied in the determination of glycogen. Decrease in final colour formation, caused by the acid used on hydrolising glycogen, was the major problem. 90 minutes incubation period
Publikováno v:
Journal of cyclic nucleotide and protein phosphorylation research. 11(2)
The stoichiometry of the phosphorylation of rabbit muscle glycogen synthase by casein/glycogen synthase kinase-1 (CK-1) depended on the concentration of protein kinase in the assay and reached values of 7-8 mol/mol subunit at high concentrations. Pho