Zobrazeno 1 - 10
of 11
pro vyhledávání: '"J H, Kenten"'
Publikováno v:
Clinical Chemistry. 38:873-879
We describe the characterization and utility of a new electrochemiluminescent (ECL) label for oligonucleotides, utilizing phosphoramidite chemistry. This phosphoramidite of the tris(2,2-bipyridine)ruthenium(II) complex, bis(2,2-bipyridine)(4-[4-(2-cy
Autor:
H P Shah, A Shah, J Peterman, Ralph A. Kamin, G F Blackburn, J Link, M J Powell, D B Talley, J Leland, J H Kenten
Publikováno v:
Clinical Chemistry. 37:1534-1539
Electrochemiluminescence (ECL) has been developed as a highly sensitive process in which reactive species are generated from stable precursors (i.e., the ECL-active label) at the surface of an electrode. This new technology has many distinct advantag
Publikováno v:
Clinical Chemistry. 37:1626-1632
We demonstrate the first use of an electrochemiluminescent (ECL) label, [4-(N-succimidyloxycarbonylpropyl)-4'-methyl-2,2'- bipyridine]ruthenium(II) dihexafluorophosphate (Origen label; IGEN Inc.), in DNA probe assays. This label allows rapid (less th
Autor:
Allan M. Weissman, Pankaj Oberoi, Howard O. Fearnhead, Shengyun Fang, D. Woods, Jane P. Jensen, Yassamin J. Safiran, J. H. Kenten, Karen H. Vousden, Ilia Davydov
Publikováno v:
Journal of biomolecular screening. 9(8)
An assay for the autoubiquitination activity of the E3 ligase HDM2 (Mdm2) was developed and adapted to a high-throughput format to identify inhibitors of this activity. The assay can also be used to measure the activity of other E3s and may be useful
Publikováno v:
Methods in molecular biology (Clifton, N.J.). 51
Publikováno v:
Clinical chemistry. 38(6)
We describe the characterization and utility of a new electrochemiluminescent (ECL) label for oligonucleotides, utilizing phosphoramidite chemistry. This phosphoramidite of the tris(2,2-bipyridine)ruthenium(II) complex, bis(2,2-bipyridine)(4-[4-(2-cy
Autor:
G F, Blackburn, H P, Shah, J H, Kenten, J, Leland, R A, Kamin, J, Link, J, Peterman, M J, Powell, A, Shah, D B, Talley
Publikováno v:
Clinical chemistry. 37(9)
Electrochemiluminescence (ECL) has been developed as a highly sensitive process in which reactive species are generated from stable precursors (i.e., the ECL-active label) at the surface of an electrode. This new technology has many distinct advantag
Publikováno v:
Clinical chemistry. 37(9)
We demonstrate the first use of an electrochemiluminescent (ECL) label, [4-(N-succimidyloxycarbonylpropyl)-4'-methyl-2,2'- bipyridine]ruthenium(II) dihexafluorophosphate (Origen label; IGEN Inc.), in DNA probe assays. This label allows rapid (less th
Autor:
Ursula Schulze-Gahmen, J H Kenten, Ian A. Wilson, Jairo H. Arevalo, Enrico A. Stura, James M. Rini
Publikováno v:
Journal of Biological Chemistry. 263:17100-17105
X-ray quality crystals which diffract to high resolution (less than or equal to 1.9-2.1 A) have been grown of an anti-peptide Fab and its complex with a 9-residue peptide antigen. Both crystals are monoclinic P2(1), with unit cell dimensions a = 90.3
Publikováno v:
Proceedings of the National Academy of Sciences. 79:6661-6665
Messenger RNA has been isolated from cells of the human myeloma line 266BL which synthesizes IgE of the myeloma ND. A fraction enriched in mRNA for the epsilon heavy chain was copied into DNA and the DNA was cloned in Escherichia coli. A chemically s