Zobrazeno 1 - 10
of 45
pro vyhledávání: '"J E Prenosil"'
Publikováno v:
Journal of Chemical Technology and Biotechnology. 31:705-709
The graphical methods of Lineweaver—Burk, Eadie, Hanes and the ‘direct linear plot’ have been used to determine the kinetic constants of β-galactosidase in soluble and immobilised states. Because some of these methods require a reciprocal conc
Publikováno v:
Biotechnology Progress. 18:1408-1413
Monitoring of cell adhesion, cell spreading, and cell proliferation opens attractive perspectives in the on-line control of monolayer cell cultures in toxicity tests, in bioreactors as used for the serial production of skin grafts, or in extracorpore
Autor:
J. E. Prenosil, Masahiro Kino-oka
Publikováno v:
Biotechnology and Bioengineering. 67:234-239
Human keratinocytes were cultured in serum-free medium for the purpose of on-line cell growth monitoring by image analysis. The validity of a process using a newly developed video microscopy system with image analysis for growth-rate monitoring in re
Autor:
J. E. Prenosil, Masahiro Kino-oka
Publikováno v:
Annals of the New York Academy of Sciences. 875:386-397
KERATOR-an automated membrane bioreactor-was developed to produce Autologous Wound Dressing (AWD) at significantly reduced cost and time of transplantation down to two weeks time. At the same time, the risk of human error is largely eliminated. The c
Autor:
P.E. Villeneuve, J. E. Prenosil
Publikováno v:
Biotechnology and Bioengineering. 59:679-683
The objective of this work was to engineer an automated system for the production of cultured epidermal autografts and sub-confluent cultured epidermal autografts. Human epidermal cells were grown directly on a transparent FEP film, which was held in
Autor:
J. E. Prenosil, Masahiro Kino-oka
Publikováno v:
IFAC Proceedings Volumes. 31:473-478
The culture conditions for production of composite skin grafts consisting of keratinocyte monolayer grown on a hydrophilic foil support were optimized. The skin grafts were produced in a special bioreactor automating the culture operations such as ce
Autor:
J. E. Prenosil, Masahiro Kino-oka
Publikováno v:
Scopus-Elsevier
Human keratinocytes were cultured on an inert polymeric film support in serum-free medium to produce subconfluent autologous skin grafts for healing of burn wounds and chronic leg ulcers. The growth of keratinocytes in Petriperm with such a hydrophil
Publikováno v:
Bioprocess Engineering. 14:51-56
Beta vulgaris cells were cultured in specially constructed fluidized bed reactors on Murashige and Skoog medium with 3% sucrose as a carbon source. Biomass density levels of approximately 17–18 g dry cell weight liter−1 for 5 liter fermenters and
Publikováno v:
Plant Cell, Tissue and Organ Culture. 43:171-183
A novel bubble free loop fluidized bed reactor for plant cell cultures was developed and tested usingCoffea arabica as a model cell line. The effects of main operational parameters like morphology and size of inoculum, oxygen supply as well as recirc
Publikováno v:
Journal of Physics D: Applied Physics. 27:1134-1138
A set-up for varying the temperature (5-90 degrees C) of planar optical waveguides useful in sensing applications is described. The principle is to control the temperature of the cover medium (in this case water). The propagation constants of guided