Zobrazeno 1 - 10
of 60
pro vyhledávání: '"J E, Churchich"'
Publikováno v:
Journal of Biological Chemistry. 267:15032-15040
Bacteriophage T7 gene 2.5 protein has been shown to interact with T7 DNA polymerase (the complex of T7 gene 5 protein and Escherichia coli thioredoxin) by affinity chromatography and fluorescence emission anisotropy. T7 DNA polymerase binds specifica
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::199b4d8ae477029a6ded91d2c412a6e6
https://doi.org/10.1016/s0021-9258(18)42142-4
https://doi.org/10.1016/s0021-9258(18)42142-4
Autor:
P Y, Cheung, J E, Churchich
Publikováno v:
The Journal of biological chemistry. 274(46)
Refolding of partially folded mitochondrial malate dehydrogenase (mMDH) is assisted by protein-disulfide isomerase (PDI). The addition of a 20-fold molar excess of PDI over denatured protein (0. 1 microM) accelerates the recovery of catalytic activit
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::04b24343eb87714d5d807326d48053b6
https://pubmed.ncbi.nlm.nih.gov/10551835
https://pubmed.ncbi.nlm.nih.gov/10551835
Publikováno v:
The international journal of biochemistrycell biology. 30(12)
Porcine brain pyridoxal kinase has been cloned. A 1.2 kilo-based cDNA with a 966-base pair open reading frame was determined from a porcine brain cortex cDNA library using PCR technique. The DNA sequence was shown to encode a protein of 322 amino aci
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::dc62b03086d7b65e41b905de0b134e81
https://pubmed.ncbi.nlm.nih.gov/9924807
https://pubmed.ncbi.nlm.nih.gov/9924807
Autor:
J E, Churchich
Publikováno v:
The Journal of biological chemistry. 272(32)
2'-Deoxy-3'-anthraniloyl adenosine-5-triphosphate (ANT-dATP) coordinated to Tb3+ was used as an environmentally sensitive probe of the nucleotide-binding site of GroEL. Tb3+.ANT-dATP recognizes the nucleotide-binding site of GroEL and inhibits ATPase
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::7a34f3c442a4c42a188e05d0bb50b32d
https://pubmed.ncbi.nlm.nih.gov/9242617
https://pubmed.ncbi.nlm.nih.gov/9242617
Publikováno v:
Biochemistry and molecular biology international. 38(4)
Several spectroscopic methods have been used to study the structure of beta-lactoglobulin B at pH 2.1 in the presence of 8M urea. Fluorescence and polarization of fluorescence spectroscopy measurements indicate that the two tryptophanyl residues of t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::c28b067114141ef7d8c0a13337f04eda
https://pubmed.ncbi.nlm.nih.gov/8728107
https://pubmed.ncbi.nlm.nih.gov/8728107
Publikováno v:
Archives of biochemistry and biophysics. 313(2)
In the presence of myo -inositol monophosphatase, terbium ions can be excited by energy transfer from the aromatic side chains of the protein. This enhancement of Tb 3+ luminescence due to its binding to the enzyme at pH 6.5 was used to determine the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6cbad2ca79d7ba454f98d35bfa3dda34
https://pubmed.ncbi.nlm.nih.gov/8080273
https://pubmed.ncbi.nlm.nih.gov/8080273
Publikováno v:
Biochemistry and molecular biology international. 32(2)
Reversible dissociation of the dimeric structure of brain myo-inositol monophosphatase into subunits was attained by the addition of guanidine-HCl (4M). The molecular mass of the subunits (29 KDa) was determined by HPLC chromatography. Separation of
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::37905704362058db84193b9d6b0e248e
https://pubmed.ncbi.nlm.nih.gov/8019438
https://pubmed.ncbi.nlm.nih.gov/8019438
Autor:
O S, Kwon, J E, Churchich
Publikováno v:
The Journal of biological chemistry. 269(1)
Fluorescence techniques have been used to investigate the interaction of bovine 70-kDa heat shock cognate protein (Hsc 70) with small molecular weight peptides and myo-inositol monophosphatase. The emission properties of Hsc 70 remain invariant upon
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::90fecd19111ac4be492602080e95002e
https://pubmed.ncbi.nlm.nih.gov/8276805
https://pubmed.ncbi.nlm.nih.gov/8276805
Autor:
T, Pineda, J E, Churchich
Publikováno v:
The Journal of biological chemistry. 268(27)
The unfolding of brain pyridoxal kinase by guanidinium HCl has been investigated at equilibrium. The overall process was reversible as judged from the complete recovery of catalytic activity after removal of guanidinium HCl. Unfolding of pyridoxal ki
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::c5ad6d58df9ff9f350bdc4eb9e1223c6
https://pubmed.ncbi.nlm.nih.gov/8376382
https://pubmed.ncbi.nlm.nih.gov/8376382
Publikováno v:
The Journal of biological chemistry. 268(11)
myo-Inositol monophosphatase isolated from pig brain is a very stable dimeric protein characterized by a rotational correlation time of 30 ns. The unfolding and dissociation of the dimeric enzyme (58 kDa) by guanidine hydrochloride have been investig
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::dbaf0ccc7e7eca3caed03cdf970341c2
https://pubmed.ncbi.nlm.nih.gov/8385126
https://pubmed.ncbi.nlm.nih.gov/8385126