Zobrazeno 1 - 10
of 19
pro vyhledávání: '"J David, Barrass"'
Publikováno v:
Mendoza ochoa, G, Barrass, J, Maudlin, I & Beggs, J 2019, ' Blocking late stages of splicing quickly limits pre-spliceosome assembly in vivo ', Rna biology . https://doi.org/10.1080/15476286.2019.1657788
RNA Biology
RNA Biology
Pre-messenger RNA splicing involves multi-step assembly of the large spliceosome complexes that catalyse the two consecutive trans-esterification reactions, resulting in intron removal. There is evidence that proof-reading mechanisms monitor the fide
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::04713d11e26e323785483db0b2b76b7c
https://doi.org/10.1080/15476286.2019.1657788
https://doi.org/10.1080/15476286.2019.1657788
Autor:
J David, Barrass, Jean D, Beggs
Publikováno v:
Journal of visualized experiments : JoVE. (150)
The nucleotide analogue, 4-thiouracil (4tU), is readily taken up by cells and incorporated into RNA as it is transcribed in vivo, allowing isolation of the RNA produced during a brief period of labelling. This is done by attaching a biotin moiety to
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::e92f073525c793c191dfe3fe90146ac7
https://pubmed.ncbi.nlm.nih.gov/31498317
https://pubmed.ncbi.nlm.nih.gov/31498317
Autor:
Jean D. Beggs, J. David Barrass
Publikováno v:
Journal of Visualized Experiments.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::9507ca64d4f24da23a832fafe169d820
https://doi.org/10.3791/59952-v
https://doi.org/10.3791/59952-v
Autor:
J David, Barrass, Gonzalo I, Mendoza-Ochoa, Isabella E, Maudlin, Emanuela, Sani, Jean D, Beggs
Publikováno v:
Journal of visualized experiments : JoVE. (149)
The plant auxin binding receptor, TIR1, recognizes proteins containing a specific auxin-inducible degron (AID) motif in the presence of auxin, targeting them for degradation. This system is exploited in many non-plant eukaryotes, such that a target p
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::4fe5a677d51be6c5bc9a293fc4fb59b9
https://pubmed.ncbi.nlm.nih.gov/31380835
https://pubmed.ncbi.nlm.nih.gov/31380835
Autor:
Jean D. Beggs, Jane E. A. Reid, Yuanhua Huang, Guido Sanguinetti, Ralph D. Hector, Sander Granneman, J. David Barrass
Publikováno v:
Barrass, J D, Reid, J E A, Huang, Y, Hector, R D, Sanguinetti, G, Beggs, J D & Granneman, S 2015, ' Transcriptome-wide RNA processing kinetics revealed using extremely short 4tU labeling ', Genome Biology, vol. 16, no. 1, pp. 282-. https://doi.org/10.1186/s13059-015-0848-1
Genome Biology
Genome Biology
Background RNA levels detected at steady state are the consequence of multiple dynamic processes within the cell. In addition to synthesis and decay, transcripts undergo processing. Metabolic tagging with a nucleotide analog is one way of determining
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::70f83fd41e42bc3ebfdf44b68ba4b796
https://hdl.handle.net/20.500.11820/cd74291e-f9ec-46d8-b788-2f66b46c36ee
https://hdl.handle.net/20.500.11820/cd74291e-f9ec-46d8-b788-2f66b46c36ee
Publikováno v:
Alexander, R D, Innocente, S A, Barrass, J D & Beggs, J D 2010, ' Splicing-Dependent RNA Polymerase Pausing in Yeast ', Molecular Cell, vol. 40, no. 4, pp. 582-593 . https://doi.org/10.1016/j.molcel.2010.11.005
In eukaryotic cells, there is evidence for functional coupling between transcription and processing of pre-mRNAs. To better understand this coupling, we performed a high-resolution kinetic analysis of transcription and splicing in budding yeast. This
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::53aca1a146c45e36258ac892a2fc8007
Autor:
Tatsiana Auchynnikava, Jean D. Beggs, Parastoo Ehsani, Richard J. Grainger, Kum-Loong Boon, Chris F. Inglehearn, J. David Barrass
Publikováno v:
Nature Structural & Molecular Biology. 14:1077-1083
Prp8 protein (Prp8p) is a highly conserved pre-mRNA splicing factor and a component of spliceosomal U5 small nuclear ribonucleoproteins (snRNPs). Although it is ubiquitously expressed, mutations in the C terminus of human Prp8p cause the retina-speci
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::00ea5c62570c8988dec04a7ea196e91a
https://doi.org/10.1038/nsmb1303
https://doi.org/10.1038/nsmb1303
Publikováno v:
Nucleic Acids Research
Pre-mRNA splicing involves two transesterification steps catalyzed by the spliceosome. How RNA substrates are positioned in each step and the molecular rearrangements involved, remain obscure. Here, we show that mutations in PRP16, PRP8, SNU114 and t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::e06988a3de8e2784963f06b0368b1321
https://pubmed.ncbi.nlm.nih.gov/25740649
https://pubmed.ncbi.nlm.nih.gov/25740649
Autor:
J. David Barrass, Jean D. Beggs
Publikováno v:
Trends in Genetics. 19:295-298
Transcriptomics, the analysis of the complement of mRNAs transcribed from a cell's genome, currently focuses mainly on mature, processed mRNAs. However, posttranscriptional processing of primary transcripts can significantly affect both the quantity
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1e3e47a69234c3cf22e1db0f415d0446
https://doi.org/10.1016/s0168-9525(03)00091-x
https://doi.org/10.1016/s0168-9525(03)00091-x
Publikováno v:
Molecular cell. 53(5)
There is good evidence for functional interactions between splicing and transcription in eukaryotes, but how and why these processes are coupled remain unknown. Prp5 protein (Prp5p) is an RNA-stimulated adenosine triphosphatase (ATPase) required for
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::221741b199a99bfd912d6e4b8bccbb29
https://pubmed.ncbi.nlm.nih.gov/24622615
https://pubmed.ncbi.nlm.nih.gov/24622615