Výsledky vyhledávání

Use of vectors to confer resistance to antibiotics g418 and hygromycin in stably transfected cell lines

Autor: R F, Santerre, J D, Walls, B W, Grinnell

Publikováno v: Methods in molecular biology (Clifton, N.J.). 7

The development of dominant selection markers to identify eukaryotic cells that have undergone a gene transformation event has greatly facilitated molecular genetic studies in higher eukaryotic cells. Selection schemes based on resistance to antibiot

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::74908e74ca0db3f2f9fad0d237470c6c
https://pubmed.ncbi.nlm.nih.gov/21416360

Zobrazit plný text záznamu

Post-translational processing events in the secretion pathway of human protein C, a complex vitamin K-dependent antithrombotic factor

Autor: J D Walls, B W Grinnell, Mcclure Don B

Publikováno v: Journal of Biological Chemistry. 267:19710-19717

Human protein C (HPC) undergoes several post-translational modifications, including gamma-carboxylation, N-linked glycosylation, and internal proteolytic processing. We have utilized a recombinant human kidney cell line (293) secreting correctly modi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::e99f97365088a26d71a6c4bf1246ab65
https://doi.org/10.1016/s0021-9258(18)41833-9

Zobrazit plný text záznamu

Characterization of a modified human tissue plasminogen activator comprising a kringle-2 and a protease domain

Autor: M W Warrick, David T. Berg, Philip J. Burck, D B McClure, B Weigel, S R Jaskunas, David H. Berg, Chris J. Vlahos, J D Walls, R M Crisel

Publikováno v: Journal of Biological Chemistry. 265:5170-5177

To study structure/function relationships of tissue plasminogen activator (t-PA) activity, one of the simplest modified t-PA structures to activate plasminogen in a fibrin-dependent manner was obtained by constructing an expression vector that delete

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::028836082f264968d07134ada1b802e2
https://doi.org/10.1016/s0021-9258(19)34102-x

Zobrazit plný text záznamu

Glycosylation of human protein C affects its secretion, processing, functional activities, and activation by thrombin

Autor: B W, Grinnell, J D, Walls, B, Gerlitz

Publikováno v: The Journal of biological chemistry. 266(15)

Human protein C (HPC) is an antithrombotic serine protease that circulates in the plasma as several glycoforms. To examine the role of glycosylation in the function of this protein, we singly eliminated each of the four potential N-linked glycosylati

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::c54a8bd40e3c99f24b92559cf6a675e9
https://pubmed.ncbi.nlm.nih.gov/2033065

Zobrazit plný text záznamu

Characterization of a modified human tissue plasminogen activator comprising a kringle-2 and a protease domain

Autor: P J, Burck, D H, Berg, M W, Warrick, D T, Berg, J D, Walls, S R, Jaskunas, R M, Crisel, B, Weigel, C J, Vlahos, D B, McClure

Publikováno v: The Journal of biological chemistry. 265(9)

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::67145fb2450e4bcc504b26d0142a44df
https://pubmed.ncbi.nlm.nih.gov/2108167

Zobrazit plný text záznamu

A rapid and versatile method for the detection and isolation of mammalian cell lines secreting recombinant proteins

Autor: J D, Walls, B W, Grinnell

Publikováno v: BioTechniques. 8(2)

The secreted-protein agarose diffusion immunoassay (SADI) described here is an efficient and time-saving method for identifying cell lines secreting recombinant protein products. In addition to significantly reducing the labor involved in identifying

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::e5125239562900249b367c1776fd778e
https://pubmed.ncbi.nlm.nih.gov/2317366

Zobrazit plný text záznamu

Vyhledávací nástroje:

Upřesnit hledání