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Ty1 In Vitro Integration: Effects of Mutations in cis and in trans

Autor: L T, Braiterman, J D, Boeke

Publikováno v: Molecular and Cellular Biology. 14:5731-5740

Mutations within the TYB gene of Ty1 encoding integrase (IN) as well as alterations in its substrate, a linear DNA molecule, were examined for their effects on in vitro IN activity, using a recently developed physical assay. Five different codon-inse

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c005deb840bf4e5dc81b1a12c3af7389
https://doi.org/10.1128/mcb.14.9.5731-5740.1994

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An In Vivo Assay for the Reverse Transcriptase of Human Retrotransposon L1 in Saccharomyces cerevisiae

Autor: B A, Dombroski, Q, Feng, S L, Mathias, D M, Sassaman, A F, Scott, H H, Kazazian, J D, Boeke

Publikováno v: Molecular and Cellular Biology. 14:4485-4492

L1 elements constitute a highly repetitive human DNA family (50,000 to 100,000 copies) lacking long terminal repeats and ending in a poly(A) tail. Some L1 elements are capable of retrotransposition in the human genome (Kazazian, H. H., Jr., C. Wong,

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5359f8f48db16c3d92eab032131d738c
https://doi.org/10.1128/mcb.14.7.4485-4492.1994

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RNA lariat debranching enzyme

Autor: S L, Ooi, C, Dann, K, Nam, D J, Leahy, M J, Damha, J D, Boeke

Publikováno v: Methods in enzymology. 342

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::e1db6a5b48a0666ee795938e062d2fcf
https://pubmed.ncbi.nlm.nih.gov/11586896

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Transcription. Is S phase important for transcriptional silencing?

Autor: J S, Smith, J D, Boeke

Publikováno v: Science (New York, N.Y.). 291(5504)

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::69b197f238b07e060fd47ea7dab8bf5e
https://pubmed.ncbi.nlm.nih.gov/11158677

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Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications

Autor: C B, Brachmann, A, Davies, G J, Cost, E, Caputo, J, Li, P, Hieter, J D, Boeke

Publikováno v: Yeast (Chichester, England). 14(2)

A set of yeast strains based on Saccharomyces cerevisiae S288C in which commonly used selectable marker genes are deleted by design based on the yeast genome sequence has been constructed and analysed. These strains minimize or eliminate the homology

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::e71343ab3ed03c7c7dd0a9b08a247ff7
https://pubmed.ncbi.nlm.nih.gov/9483801

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A useful colony colour phenotype associated with the yeast selectable/counter-selectable marker MET15

Autor: G J, Cost, J D, Boeke

Publikováno v: Yeast (Chichester, England). 12(10)

Strains of Saccharomyces cerevisiae bearing null alleles of the met15 gene are methionine auxotrophs and become darkly pigmented in the presence of Pb2+ ions (Ono et al. (1991). Appl. Env. Microbiol. 57, 3183-3186). We describe the cloning of a usefu

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::f17b3e1f26e55d57eb6702018ca98d3c
https://pubmed.ncbi.nlm.nih.gov/8873447

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Yeast lariat debranching enzyme. Substrate and sequence specificity

Autor: K, Nam, R H, Hudson, K B, Chapman, K, Ganeshan, M J, Damha, J D, Boeke

Publikováno v: The Journal of biological chemistry. 269(32)

Yeast RNA lariat debranching enzyme has been purified to near homogeneity using a bacterial overproducer of the enzyme. The enzyme is capable of digesting a variety of branched nucleic acid substrates, including group II intron lariats, multicopy sin

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::237a0652a68e19b17407e9b782180595
https://pubmed.ncbi.nlm.nih.gov/7519612

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