Transient, Consequential Increases in Extracellular Potassium Ions Accompany Channelrhodopsin2 Excitation

Autor: J. Christopher Octeau, Mohitkumar R. Gangwani, Sushmita L. Allam, Duy Tran, Shuhan Huang, Tuan M. Hoang-Trong, Peyman Golshani, Timothy H. Rumbell, James R. Kozloski, Baljit S. Khakh

Publikováno v: Cell Reports, Vol 27, Iss 8, Pp 2249-2261.e7 (2019)

Summary: Channelrhodopsin2 (ChR2) optogenetic excitation is widely used to study neurons, astrocytes, and circuits. Using complementary approaches in situ and in vivo, we found that ChR2 stimulation leads to significant transient elevation of extrace

Externí odkaz: https://doaj.org/article/c1aa3074aba74728a3a6e75d06f3e790

G protein beta 5 is targeted to D2-dopamine receptor-containing biochemical compartments and blocks dopamine-dependent receptor internalization.

Autor: J Christopher Octeau, Joseph M Schrader, Ikuo Masuho, Meenakshi Sharma, Christopher Aiudi, Ching-Kang Chen, Abraham Kovoor, Jeremy Celver

Publikováno v: PLoS ONE, Vol 9, Iss 8, p e105791 (2014)

G beta 5 (Gbeta5, Gβ5) is a unique G protein β subunit that is thought to be expressed as an obligate heterodimer with R7 regulator of G protein signaling (RGS) proteins instead of with G gamma (Gγ) subunits. We found that D2-dopamine receptor (D2

Externí odkaz: https://doaj.org/article/57c65d263ef84be59288b515d21880a6

Assessing Neuron–Astrocyte Spatial Interactions Using the Neuron–Astrocyte Proximity Assay

Autor: Baljit S. Khakh, Mark H. G. Verheijen, J. Christopher Octeau, Aina Badia‐Soteras

Publikováno v: Badia-Soteras, A, Octeau, J C, Verheijen, M H G & Khakh, B S 2020, ' Assessing Neuron-Astrocyte Spatial Interactions Using the Neuron-Astrocyte Proximity Assay ', Current protocols in neuroscience, vol. 91, no. 1, e91, pp. e91 . https://doi.org/10.1002/cpns.91
Current protocols in neuroscience, 91(1):e91

Astrocytes are morphologically complex cells with numerous close contacts with neurons at the level of their somata, branches, and branchlets. The smallest astrocyte processes make discrete contacts with synapses at scales that cannot be observed by

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::88303d8cd91da25f597ec1a51887bd59
https://doi.org/10.1002/cpns.91

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Making, Testing, and Using Potassium Ion Selective Microelectrodes in Tissue Slices of Adult Brain

Autor: J. Christopher Octeau, Guido C. Faas, Istvan Mody, Baljit S. Khakh

Publikováno v: Journal of Visualized Experiments.

Potassium ions significantly contribute to the resting membrane potential of cells and, therefore, extracellular K(+) concentration is a crucial regulator of cell excitability. Altered concentrations of extracellular K(+) affect the resting membrane

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::36dd47da91839cb150793c22dbd32df3
https://doi.org/10.3791/57511