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Autor:
J A Jagow, Karen C. Jinneman, Curtis J. Omiecinski, Stephen D. Weagant, Walter E. Hill, Charles A. Kaysner
Publikováno v:
Journal of Food Protection. 62:438-443
By including digoxigenin-11-dUTP in a polymerase chain reaction (PCR), amplification products were produced that contained nonisotopic markers for use as DNA hybridization probes. Because these labeled amplicons encode pathogenic traits for specific
The polymerase chain reaction (PCR) was used to amplify a 760-base-pair (bp) fragment with the 220-kbp invasive plasmids of enteroinvasive Escherichia coli, Shigella flexneri, Shigella dysenteriae, Shigella boydii, and Shigella sonnei as templates. T
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5dad610b45ba21506a6a8be2db152954
https://europepmc.org/articles/PMC184467/
https://europepmc.org/articles/PMC184467/
Publikováno v:
Microbial Toxins in Foods and Feeds ISBN: 9781461279167
Enteroinvasive Escherichia coli and the four Shigella species, S. dysenteriae, S. flexneri, S. sonnei and S. boydii are the causative agents of bacillary dysentery (shigellosis) in higher primates. Shigella-induced infections are due to the ingestion
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::d632b25af28eea3453ee6bd07c63e122
https://doi.org/10.1007/978-1-4613-0663-4_11
https://doi.org/10.1007/978-1-4613-0663-4_11
Autor:
J A Jagow, Walter E. Hill
Publikováno v:
Molecular and Cellular Probes. 2:189-195
The efficiency of enumerating virulent Yersinia enterocolitica colonies was determined for 10 artificially contaminated foods by autoradiography after DNA colony hybridization using a 32 P-labelled genetic probe and paper filters. Enumeration efficie
Autor:
R J Barnard, A. H. Schwab, B. A. Wentz, J. M. Lanier, A. P. Duran, R. B. Read, J A Jagow, A. Swartzentruber
Publikováno v:
Journal of Food Protection. 48:70-75
In-line samples of crust, filling and topping were collected from pies being prepared by all U.S. firms making frozen cream-type pies for interstate distribution. All firms adhered to Good Manufacturing Practices, as determined by visual inspection.
Publikováno v:
Journal of AOAC INTERNATIONAL. 69:531-536
The genes that encode several of the enterotoxins produced by Escherichia coli have been cloned by recombinant DNA techniques. When the nucleotide sequence of these genes is determined, defined sequence oligonucleotides that include a part of these g
Autor:
B K Boutin, Joseph M. Madden, J A Jagow, B. A. McCardell, W L Payne, D.B. Shah, Walter E. Hill
Publikováno v:
Applied and Environmental Microbiology. 45:1324-1330
Four methods were compared for detecting heat-labile toxin production by Escherichia coli: DNA colony hybridization, two enzyme-linked immunosorbent assays, and the mouse Y-1 adrenal cell reaction. Although results of the methods were in general agre
Autor:
S L Moseley, J L Ferreira, J A Jagow, Gemski P, Noah Cw, Davis Vm, W L Payne, Walter E. Hill, English Ll, Crouch Rj
Publikováno v:
Journal of AOAC INTERNATIONAL. 67:801-807
Enteropathogenic Escherichia coli strains may produce a cholera-like, heat-labile enterotoxin (LT) as a virulence factor. The gene that codes for LT can be purified by recombinant DNA techniques and used as a genetic probe for DNA hybridization. Thes
Autor:
W E, Hill, W L, Payne, R J, Crouch, V M, Davis, L L, English, J L, Ferreira, P, Gemski, J A, Jagow, S L, Moseley, C W, Noah
Publikováno v:
Journal - Association of Official Analytical Chemists. 67(4)
Enteropathogenic Escherichia coli strains may produce a cholera-like, heat-labile enterotoxin (LT) as a virulence factor. The gene that codes for LT can be purified by recombinant DNA techniques and used as a genetic probe for DNA hybridization. Thes