Zobrazeno 1 - 10
of 12
pro vyhledávání: '"J A, Wehrly"'
Publikováno v:
Methods in enzymology. 184
Publikováno v:
Clinical Chemistry. 29:609-613
We describe assays for functional antithrombin III (AT III) and plasminogen in plasma with the Du Pont aca discrete clinical analyzer. Both are two-stage kinetic assays, based on synthetic substrate methodologies, and require 20-microL sample volumes
Autor:
J. A. Wehrly, J. F. Cannon, Stuart W. Peltz, Walter F. Mangel, John R. Brocklehurst, D. C. Livingston, Steven P. Leytus, Gary Peltz
Publikováno v:
Biochemistry. 20:4298-4306
The molecule 3',6'-bis(4-guanidinobenzoyloxy)-5-[N'-(4-carboxyphenyl)thioureido[spirop]isobenzofuran-1-(3H),9'-[9H]xanthen]-3-one, abbreviated FDE, was designed and synthesized as a fluorogenic active-site titrant for serine proteases. It is an analo
Publikováno v:
Clinical Chemistry. 29:614-617
A method for determination of functional fibrinogen has been developed for the Du Pont aca discrete clinical analyzer. This fully automated assay is based on the rate of fibrin turbidity formation when thrombin is added to the test sample. The rate o
Publikováno v:
Clinical Chemistry. 29:603-608
Optimized assays for antithrombin III and plasminogen have been developed based on a study of the kinetic parameters Km and Kcat for four commercially available substrates: the p-nitroanilide derivatives of D-Phe-pipecolyl-Arg (S-2238), and toluenesu
Publikováno v:
Clinical chemistry. 29(4)
A method for determination of functional fibrinogen has been developed for the Du Pont aca discrete clinical analyzer. This fully automated assay is based on the rate of fibrin turbidity formation when thrombin is added to the test sample. The rate o
Publikováno v:
Clinical chemistry. 29(4)
Optimized assays for antithrombin III and plasminogen have been developed based on a study of the kinetic parameters Km and Kcat for four commercially available substrates: the p-nitroanilide derivatives of D-Phe-pipecolyl-Arg (S-2238), and toluenesu
Publikováno v:
Oral Presentations.
The need for adherence to basic enzyme kinetics principles in the measurement of coagulation enzymes with synthetic substrates has recently been addressed by the ICTH Subcommittee on Synthetic Substrates. We describe an assay for anti thrombin III (A
Publikováno v:
Clinical chemistry. 29(4)
We describe assays for functional antithrombin III (AT III) and plasminogen in plasma with the Du Pont aca discrete clinical analyzer. Both are two-stage kinetic assays, based on synthetic substrate methodologies, and require 20-microL sample volumes
Autor:
John R. Brocklehurst, H.-Y. Liu, B. L. Salter, Gary Peltz, Steven P. Leytus, Walter F. Mangel, D. C. Livingston, J. A. Wehrly, J. L. Mosher, J. F. Cannon
Publikováno v:
Cold Spring Harbor symposia on quantitative biology.