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Cytostatic p21 G protein-activated protein kinase gamma-PAK

Autor: J, Roig, J A, Traugh

Publikováno v: Vitamins and hormones. 62

The p21-activated protein kinase gamma-PAK, also known as PAK2, has very different properties from the other two highly conserved isoforms of the PAK family, alpha-PAK (PAK1) and beta-PAK (PAK3). gamma-PAK has cytostatic activity, as shown by inhibit

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::7c056204915834db0f34aa907c781729
https://pubmed.ncbi.nlm.nih.gov/11345898

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Substrates enhance autophosphorylation and activation of p21-activated protein kinase gamma-PAK in the absence of activation loop phosphorylation

Autor: R, Jakobi, Z, Huang, B N, Walter, P T, Tuazon, J A, Traugh

Publikováno v: European journal of biochemistry. 267(14)

The p21-activated protein kinase gamma-PAK from rabbit, expressed in insect cells, is activated following binding of Cdc42(GTPgammaS). The rate of autophosphorylation is increased fivefold and the protein kinase activity 13-fold, as measured with the

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::062d1141f3bb350ac88e2f888a19203d
https://pubmed.ncbi.nlm.nih.gov/10880965

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p21-activated protein kinase gamma-PAK is translocated and activated in response to hyperosmolarity. Implication of Cdc42 and phosphoinositide 3-kinase in a two-step mechanism for gamma-PAK activation

Autor: J, Roig, Z, Huang, C, Lytle, J A, Traugh

Publikováno v: The Journal of biological chemistry. 275(22)

A member of the family of p21-activated protein kinases, gamma-PAK, has cytostatic properties and is activated during apoptosis and in response to DNA damage. To determine whether gamma-PAK is activated by other types of cell stress and to assess its

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::ccef2b805b13827f072380f634f5d459
https://pubmed.ncbi.nlm.nih.gov/10748040

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p21-activated protein kinase gamma-PAK is activated by ionizing radiation and other DNA-damaging agents. Similarities and differences to alpha-PAK

Autor: J, Roig, J A, Traugh

Publikováno v: The Journal of biological chemistry. 274(44)

The p21-activated protein kinase gamma-PAK is activated 2-5-fold in response to ionizing radiation (IR) in 3T3-L1 fibroblasts and U937 leukemia cells. gamma-PAK is activated in a dose- and time-dependent manner. Doses from 1 to 100 Gy result in signi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::53d3328daf99b92db59a83f163a9a563
https://pubmed.ncbi.nlm.nih.gov/10531298

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A structural model for elongation factor 1 (EF-1) and phosphorylation by protein kinase CKII

Autor: G T, Sheu, J A, Traugh

Publikováno v: Molecular and cellular biochemistry. 191(1-2)

EF-1alpha binds aminoacyl-tRNA to the ribosome with the hydrolysis of GTP; the betagammadelta complex facilitates the exchange of GDP for GTP to initiate another round of elongation. To examine the subunit structure of EF-1 and phosphorylation by pro

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::55265aa9c261f65e41012500e08cf69d
https://pubmed.ncbi.nlm.nih.gov/10094407

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Recombinant subunits of mammalian elongation factor 1 expressed in Escherichia coli. Subunit interactions, elongation activity, and phosphorylation by protein kinase CKII

Autor: G T, Sheu, J A, Traugh

Publikováno v: The Journal of biological chemistry. 272(52)

The first step in elongation requires two different activities; elongation factor (EF)-1alpha transfers aminoacyl-tRNA to the ribosome and is released upon hydrolysis of GTP, EF-1betagammadelta catalyzes exchange of GDP on EF-1alpha with GTP. To anal

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::39f4126771d878cdc167b49c4f279bbc
https://pubmed.ncbi.nlm.nih.gov/9407120

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Renaturation and reconstitution of functional holoenzyme from recombinant subunits of casein kinase II expressed as inclusion bodies in E. coli

Autor: W J, Lin, R, Jakobi, J A, Traugh

Publikováno v: Genetic engineering. 18

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::69ee2191c05945fe770eae6469e6a2f9
https://pubmed.ncbi.nlm.nih.gov/8785116

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Analysis of the ATP/GTP binding site of casein kinase II by site-directed mutagenesis

Autor: R, Jakobi, J A, Traugh

Publikováno v: Physiological chemistry and physics and medical NMR. 27(4)

Casein kinase II is one of only a few protein kinases which effectively utilize ATP and GTP in the phosphotransferase reaction. Two residues conserved in the ATP binding domain of other protein kinases are unique to the catalytic (alpha) subunit of c

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::9d7d4d77db6b08b43558d7379fe2a460
https://pubmed.ncbi.nlm.nih.gov/8768785

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Serum and insulin regulate cap function in 3T3-L1 cells

Autor: D R, Gallie, J A, Traugh

Publikováno v: The Journal of biological chemistry. 269(10)

Serum starvation and readdition of serum, insulin, or mitogens result in changes in phosphorylation of several eukaryotic initiation factors that correlate with a decrease or stimulation in translation, respectively. We have introduced in vitro synth

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::d7b7666787066fc55242e8b2ed29f1d1
https://pubmed.ncbi.nlm.nih.gov/8125928

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