Zobrazeno 1 - 10
of 164
pro vyhledávání: '"J A, Spaan"'
Autor:
Willy J. M. Spaan, David A. Brian
Publikováno v:
Seminars in Virology
Naturally occurring defective interfering RNAs have been found in 4 of 14 coronavirus species. They range in size from 2.2 kb to approximately 25 kb, or 80% of the 30-kb parent virus genome. The large DI RNAs do not in all cases appear to require hel
Autor:
Chris Lauber, Kalina T. Zlateva, Willy J. M. Spaan, Alexander E. Gorbalenya, Alexander A. Kravchenko, Eric C. J. Claas, Kelly M. Crusio, Andrey M. Leontovich
Publikováno v:
Journal of Virological Methods
Journal of Virological Methods, 177(2), 174-183
Journal of Virological Methods, 177(2), 174-183
Highlights ► We developed one-step RT-PCR assay to recognize corona- and toroviruses. ► It utilizes consensus-degenerated hybrid oligonucleotide primers. ► The primers were targeted to RNA-dependent RNA polymerase locus. ► Assay recognized vi
Autor:
Erik W.A. Marijt, Aloys C.M. Kroes, Eric C. J. Claas, Caroline S. van der Blij-de Brouwer, Christa Nederstigt, Willy J. M. Spaan, Pim L.J. van der Heiden, Ann C. T. M. Vossen, Clara C. Posthuma, Martha T. van der Beek, Eric J. Snijder
Publikováno v:
Journal of Clinical Virology
Journal of Clinical Virology, 51(1), 25-30
Journal of Clinical Virology, 51(1), 25-30
BACKGROUND Persistent infections with herpesviruses such as human cytomegalovirus (HCMV) frequently occur after solid organ or stem cell transplantation, and are due to either failure of the host to immunologically control the virus or emerging resis
Publikováno v:
Journal of Virology
Virus infection in vitro can either result in a cytopathic effect (CPE) or proceed without visible changes in infected cells (noncytopathic infection). We are interested in understanding the mechanisms controlling the impact of coronavirus infection
Publikováno v:
Journal of Virology
Recently, a paper was published in which it was proposed that the GxxxG motif of the severe acute respiratory syndrome (SARS) coronavirus spike (S) protein transmembrane domain plays a vital role in oligomerization of the protein (E. Arbely, Z. Grano
Publikováno v:
Virology
Many viruses encode antagonists to prevent interferon (IFN) induction. Infection of fibroblasts with the murine hepatitis coronavirus (MHV) and SARS-coronavirus (SARS-CoV) did not result in nuclear translocation of interferon-regulatory factor 3 (IRF
Publikováno v:
Journal of Virology
The spike protein (S) of severe acute respiratory syndrome coronavirus (SARS-CoV) is responsible for receptor binding and membrane fusion. It contains a highly conserved transmembrane domain that consists of three parts: an N-terminal tryptophan-rich
Autor:
Kathleen M. Brasky, Jean L. Patterson, Ilia Tikhonov, Richard Molenkamp, Vincent Deubel, Anysha Ticer, Igor S. Lukashevich, Peter J. Bredenbeek, Phillippe Marianneau, Ricardo Carrion, Juan C. Zapata, Dmitry Moshkoff, Maria S. Salvato, Willy J. M. Spaan
Publikováno v:
Virology. 345:299-304
The Yellow Fever Vaccine 17D (YFV17D) has been used as a vector for the Lassa virus glycoprotein precursor (LASV-GPC) resulting in construction of YFV17D/LASV-GPC recombinant virus. The virus was replication-competent and processed the LASV-GPC in ce
Publikováno v:
Journal of general virology. 87(Part 6):1403-1421
Many positive-stranded RNA viruses use subgenomic mRNAs to express part of their genetic information. To produce structural and accessory proteins, members of the order Nidovirales (corona-, toro-, arteri- and roniviruses) generate a 3′ co-terminal
Publikováno v:
Virology
Virology, 167(2), 370. Elsevier
Virology, 167(2), 370. Elsevier
The genetic information, carried on mRNA 6 of feline infectious peritonitis virus (FIPV) strain 79–1146, was determined by sequence analysis of cDNA clones derived from the 3′ end of the FIPV genome. Two ORFs were found, encoding polypeptides of