Zobrazeno 1 - 10
of 163
pro vyhledávání: '"Jürgen M Plitzko"'
Autor:
Maud Dumoux, Thomas Glen, Jake LR Smith, Elaine ML Ho, Luis MA Perdigão, Avery Pennington, Sven Klumpe, Neville BY Yee, David Andrew Farmer, Pui YA Lai, William Bowles, Ron Kelley, Jürgen M Plitzko, Liang Wu, Mark Basham, Daniel K Clare, C Alistair Siebert, Michele C Darrow, James H Naismith, Michael Grange
Publikováno v:
eLife, Vol 12 (2023)
Serial focussed ion beam scanning electron microscopy (FIB/SEM) enables imaging and assessment of subcellular structures on the mesoscale (10 nm to 10 µm). When applied to vitrified samples, serial FIB/SEM is also a means to target specific structur
Externí odkaz:
https://doaj.org/article/fbc6e73bd5344bb9801714368bad32dd
Autor:
Daan B Boltje, Jacob P Hoogenboom, Arjen J Jakobi, Grant J Jensen, Caspar TH Jonker, Max J Kaag, Abraham J Koster, Mart GF Last, Cecilia de Agrela Pinto, Jürgen M Plitzko, Stefan Raunser, Sebastian Tacke, Zhexin Wang, Ernest B van der Wee, Roger Wepf, Sander den Hoedt
Publikováno v:
eLife, Vol 11 (2022)
Cryogenic electron tomography (cryo-ET) combined with subtomogram averaging, allows in situ visualization and structure determination of macromolecular complexes at subnanometre resolution. Cryogenic focused ion beam (cryo-FIB) micromachining is used
Externí odkaz:
https://doaj.org/article/83cd08d1d010476f869e35008b71c55b
Autor:
Matthias Pöge, Julia Mahamid, Sanae S Imanishi, Jürgen M Plitzko, Krzysztof Palczewski, Wolfgang Baumeister
Publikováno v:
eLife, Vol 10 (2021)
The unique membrane organization of the rod outer segment (ROS), the specialized sensory cilium of rod photoreceptor cells, provides the foundation for phototransduction, the initial step in vision. ROS architecture is characterized by a stack of ide
Externí odkaz:
https://doaj.org/article/e7681bcf4abe4cd2827282275f70373e
Autor:
Sven Klumpe, Herman KH Fung, Sara K Goetz, Ievgeniia Zagoriy, Bernhard Hampoelz, Xiaojie Zhang, Philipp S Erdmann, Janina Baumbach, Christoph W Müller, Martin Beck, Jürgen M Plitzko, Julia Mahamid
Publikováno v:
eLife, Vol 10 (2021)
Lamella micromachining by focused ion beam milling at cryogenic temperature (cryo-FIB) has matured into a preparation method widely used for cellular cryo-electron tomography. Due to the limited ablation rates of low Ga+ ion beam currents required to
Externí odkaz:
https://doaj.org/article/dac8598bb5fe4b05b4258e93085e26ee
Charting the native architecture of Chlamydomonas thylakoid membranes with single-molecule precision
Autor:
Wojciech Wietrzynski, Miroslava Schaffer, Dimitry Tegunov, Sahradha Albert, Atsuko Kanazawa, Jürgen M Plitzko, Wolfgang Baumeister, Benjamin D Engel
Publikováno v:
eLife, Vol 9 (2020)
Thylakoid membranes scaffold an assortment of large protein complexes that work together to harness the energy of light. It has been a longstanding challenge to visualize how the intricate thylakoid network organizes these protein complexes to finely
Externí odkaz:
https://doaj.org/article/fce8ee317d8e424ca6c74db803a864e2
Autor:
Yury S Bykov, Miroslava Schaffer, Svetlana O Dodonova, Sahradha Albert, Jürgen M Plitzko, Wolfgang Baumeister, Benjamin D Engel, John AG Briggs
Publikováno v:
eLife, Vol 6 (2017)
COPI-coated vesicles mediate trafficking within the Golgi apparatus and from the Golgi to the endoplasmic reticulum. The structures of membrane protein coats, including COPI, have been extensively studied with in vitro reconstitution systems using pu
Externí odkaz:
https://doaj.org/article/5c206a923cfc4cecbe5ecf116b147a82
Publikováno v:
PLoS Genetics, Vol 12, Iss 6, p e1006101 (2016)
Magnetosomes of magnetotactic bacteria contain well-ordered nanocrystals for magnetic navigation and have recently emerged as the most sophisticated model system to study the formation of membrane bounded organelles in prokaryotes. Magnetosome biosyn
Externí odkaz:
https://doaj.org/article/f5bc345549184bb4a36cddcd225bcfd9
Autor:
Benjamin D Engel, Miroslava Schaffer, Luis Kuhn Cuellar, Elizabeth Villa, Jürgen M Plitzko, Wolfgang Baumeister
Publikováno v:
eLife, Vol 4 (2015)
Externí odkaz:
https://doaj.org/article/53d6108343714cc88148b3d0fe7e6fa3
Autor:
Benjamin D Engel, Miroslava Schaffer, Luis Kuhn Cuellar, Elizabeth Villa, Jürgen M Plitzko, Wolfgang Baumeister
Publikováno v:
eLife, Vol 4 (2015)
Chloroplast function is orchestrated by the organelle's intricate architecture. By combining cryo-focused ion beam milling of vitreous Chlamydomonas cells with cryo-electron tomography, we acquired three-dimensional structures of the chloroplast in i
Externí odkaz:
https://doaj.org/article/aa7ddddf6379430a81750374cc73fc4c
Autor:
Philipp S. Erdmann, Zhen Hou, Sven Klumpe, Sagar Khavnekar, Florian Beck, Florian Wilfling, Jürgen M. Plitzko, Wolfgang Baumeister
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-9 (2021)
The large and small subunits of the ribosome are synthesized independently within the nucleolus — a membrane-less compartment within the nucleus — before being exported into the cytoplasm. Here, the authors use in situ cryo-ET to observe ribosome
Externí odkaz:
https://doaj.org/article/29af8d2ce12c4b48a297291bce32bf53