Zobrazeno 1 - 10
of 199
pro vyhledávání: '"Jürgen M Plitzko"'
Autor:
Maud Dumoux, Thomas Glen, Jake LR Smith, Elaine ML Ho, Luis MA Perdigão, Avery Pennington, Sven Klumpe, Neville BY Yee, David Andrew Farmer, Pui YA Lai, William Bowles, Ron Kelley, Jürgen M Plitzko, Liang Wu, Mark Basham, Daniel K Clare, C Alistair Siebert, Michele C Darrow, James H Naismith, Michael Grange
Publikováno v:
eLife, Vol 12 (2023)
Serial focussed ion beam scanning electron microscopy (FIB/SEM) enables imaging and assessment of subcellular structures on the mesoscale (10 nm to 10 µm). When applied to vitrified samples, serial FIB/SEM is also a means to target specific structur
Externí odkaz:
https://doaj.org/article/fbc6e73bd5344bb9801714368bad32dd
Autor:
Daan B Boltje, Jacob P Hoogenboom, Arjen J Jakobi, Grant J Jensen, Caspar TH Jonker, Max J Kaag, Abraham J Koster, Mart GF Last, Cecilia de Agrela Pinto, Jürgen M Plitzko, Stefan Raunser, Sebastian Tacke, Zhexin Wang, Ernest B van der Wee, Roger Wepf, Sander den Hoedt
Publikováno v:
eLife, Vol 11 (2022)
Cryogenic electron tomography (cryo-ET) combined with subtomogram averaging, allows in situ visualization and structure determination of macromolecular complexes at subnanometre resolution. Cryogenic focused ion beam (cryo-FIB) micromachining is used
Externí odkaz:
https://doaj.org/article/83cd08d1d010476f869e35008b71c55b
Autor:
Philipp S. Erdmann, Zhen Hou, Sven Klumpe, Sagar Khavnekar, Florian Beck, Florian Wilfling, Jürgen M. Plitzko, Wolfgang Baumeister
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-9 (2021)
The large and small subunits of the ribosome are synthesized independently within the nucleolus — a membrane-less compartment within the nucleus — before being exported into the cytoplasm. Here, the authors use in situ cryo-ET to observe ribosome
Externí odkaz:
https://doaj.org/article/29af8d2ce12c4b48a297291bce32bf53
Autor:
Laura Burbaum, Jonathan Schneider, Sarah Scholze, Ralph T. Böttcher, Wolfgang Baumeister, Petra Schwille, Jürgen M. Plitzko, Marion Jasnin
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-12 (2021)
Sarcomeres, the building blocks of striated muscles, comprise ordered actomyosin arrays involved in force production. Here, the authors visualize sarcomere organization in neonatal cardiomyocytes with in situ cryo-electron tomography, revealing a red
Externí odkaz:
https://doaj.org/article/e07d093c4c6e4c35be0d0751adcc87f0
Autor:
Matthias Pöge, Julia Mahamid, Sanae S Imanishi, Jürgen M Plitzko, Krzysztof Palczewski, Wolfgang Baumeister
Publikováno v:
eLife, Vol 10 (2021)
The unique membrane organization of the rod outer segment (ROS), the specialized sensory cilium of rod photoreceptor cells, provides the foundation for phototransduction, the initial step in vision. ROS architecture is characterized by a stack of ide
Externí odkaz:
https://doaj.org/article/e7681bcf4abe4cd2827282275f70373e
Autor:
Sven Klumpe, Herman KH Fung, Sara K Goetz, Ievgeniia Zagoriy, Bernhard Hampoelz, Xiaojie Zhang, Philipp S Erdmann, Janina Baumbach, Christoph W Müller, Martin Beck, Jürgen M Plitzko, Julia Mahamid
Publikováno v:
eLife, Vol 10 (2021)
Lamella micromachining by focused ion beam milling at cryogenic temperature (cryo-FIB) has matured into a preparation method widely used for cellular cryo-electron tomography. Due to the limited ablation rates of low Ga+ ion beam currents required to
Externí odkaz:
https://doaj.org/article/dac8598bb5fe4b05b4258e93085e26ee
Charting the native architecture of Chlamydomonas thylakoid membranes with single-molecule precision
Autor:
Wojciech Wietrzynski, Miroslava Schaffer, Dimitry Tegunov, Sahradha Albert, Atsuko Kanazawa, Jürgen M Plitzko, Wolfgang Baumeister, Benjamin D Engel
Publikováno v:
eLife, Vol 9 (2020)
Thylakoid membranes scaffold an assortment of large protein complexes that work together to harness the energy of light. It has been a longstanding challenge to visualize how the intricate thylakoid network organizes these protein complexes to finely
Externí odkaz:
https://doaj.org/article/fce8ee317d8e424ca6c74db803a864e2
Autor:
Daniel Bollschweiler, Laura Radu, Luay Joudeh, Jürgen M. Plitzko, Robert M. Henderson, Ioanna Mela, Luca Pellegrini
Publikováno v:
Open Biology, Vol 9, Iss 10 (2019)
The synaptonemal complex (SC) keeps homologous chromosomes in close alignment during meiotic recombination. A hallmark of the SC is the presence of its constituent protein SYCP3 on the chromosome axis. During SC assembly, SYCP3 is deposited on both a
Externí odkaz:
https://doaj.org/article/4e53c5cc64c74d1da7694973f3d8e706
Autor:
Shyamal Mosalaganti, Jan Kosinski, Sahradha Albert, Miroslava Schaffer, Daniela Strenkert, Patrice A. Salomé, Sabeeha S. Merchant, Jürgen M. Plitzko, Wolfgang Baumeister, Benjamin D. Engel, Martin Beck
Publikováno v:
Nature Communications, Vol 9, Iss 1, Pp 1-8 (2018)
While the architecture of vertebrate nuclear pore complexes (NPCs) is well understood, the extent of its evolutionary conservation is still unclear. Here, the authors analyze the in situ architecture of an algal NPC, revealing distinct structural fea
Externí odkaz:
https://doaj.org/article/d5b2c6bf446f4dc1bf1a94753ef03049
Autor:
Jürgen M Plitzko, Sven Klumpe, Oda H Schioetz, Anna Bieber, Cristina Capitanio, Jakub Kuba, Alexander Rigort
Publikováno v:
Microscopy and Microanalysis. 28:1292-1295