Zobrazeno 1 - 10
of 285
pro vyhledávání: '"Jürgen Hubbuch"'
Publikováno v:
Frontiers in Bioengineering and Biotechnology, Vol 12 (2024)
The conjugation reaction is the central step in the manufacturing process of antibody-drug conjugates (ADCs). This reaction generates a heterogeneous and complex mixture of differently conjugated sub-species depending on the chosen conjugation chemis
Externí odkaz:
https://doaj.org/article/0bf1f60593834f988cd1f6a6b7047471
Autor:
Adrian Schimek, Judy K.M. Ng, Ioannes Basbas, Fabian Martin, Dongyue Xin, David Saleh, Jürgen Hubbuch
Publikováno v:
Molecular Therapy: Methods & Clinical Development, Vol 32, Iss 4, Pp 101335- (2024)
Externí odkaz:
https://doaj.org/article/e2415659fad24882ba916afd0500c2aa
Publikováno v:
Frontiers in Bioengineering and Biotechnology, Vol 12 (2024)
Virus-like particles (VLPs) are a promising class of biopharmaceuticals for vaccines and targeted delivery. Starting from clarified lysate, VLPs are typically captured by selective precipitation. While VLP precipitation is induced by step-wise or con
Externí odkaz:
https://doaj.org/article/781a4b0952a94375b029e37c6b58fcfd
Autor:
Christina Henriette Wegner, Sebastian Mathis Eming, Brigitte Walla, Daniel Bischoff, Dirk Weuster-Botz, Jürgen Hubbuch
Publikováno v:
Frontiers in Bioengineering and Biotechnology, Vol 12 (2024)
Protein crystallization as opposed to well-established chromatography processes has the benefits to reduce production costs while reaching a comparable high purity. However, monitoring crystallization processes remains a challenge as the produced cry
Externí odkaz:
https://doaj.org/article/fa4a1b828ff5499aa3726e5163379ec0
Publikováno v:
Frontiers in Bioengineering and Biotechnology, Vol 12 (2024)
Chemometric modeling for spectral data is considered a key technology in biopharmaceutical processing to realize real-time process control and release testing. Machine learning (ML) models have been shown to increase the accuracy of various spectral
Externí odkaz:
https://doaj.org/article/1d6deebc14b142d98d4769d585f3ad23
Autor:
Jakob Heckel, Andres Martinez, Carsten Elger, Markus Haindl, Michael Leiss, Raphael Ruppert, Chris Williams, Jürgen Hubbuch, Tobias Graf
Publikováno v:
Molecular Therapy: Methods & Clinical Development, Vol 31, Iss , Pp 101148- (2023)
Recombinant adeno-associated viruses (rAAVs) are promising gene delivery vectors in the emerging field of in vivo gene therapies. To ensure their consistent quality during manufacturing and process development, multiple analytical techniques have bee
Externí odkaz:
https://doaj.org/article/6750eb5c387241d1ae66c324c8f288ed
Publikováno v:
Frontiers in Bioengineering and Biotechnology, Vol 11 (2023)
Externí odkaz:
https://doaj.org/article/656ca6e9c2d74609b936ddefebd5f930
Autor:
Nils Hillebrandt, Jürgen Hubbuch
Publikováno v:
Frontiers in Bioengineering and Biotechnology, Vol 11 (2023)
Non-enveloped virus-like particles (VLPs) are versatile protein nanoparticles with great potential for biopharmaceutical applications. However, conventional protein downstream processing (DSP) and platform processes are often not easily applicable du
Externí odkaz:
https://doaj.org/article/8b461955ba694c14aecfc6d39e17d03f
Publikováno v:
Frontiers in Bioengineering and Biotechnology, Vol 11 (2023)
The manufacturing of antibody-drug conjugates (ADCs) involves the addition of a cytotoxic small-molecule linker-drug (= payload) to a solution of functionalized antibodies. For the development of robust conjugation processes, initially small-scale re
Externí odkaz:
https://doaj.org/article/f4db704a20414ae2a319691d2ba694d8
Publikováno v:
Viruses, Vol 16, Iss 1, p 13 (2023)
Effective process development towards intensified processing for gene delivery applications using Hepatitis B core Antigen (HBcAg) virus-like particles (VLPs) relies on analytical methods for the absolute quantification of HBcAg VLP proteins and boun
Externí odkaz:
https://doaj.org/article/0227ef6e33c44625a7cd96e2f8ab2bed