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pro vyhledávání: '"Jörgen, Samuelsson"'
Ion-pair chromatography is the de facto standard for separating oligonucleotides and related impurities, particularly for analysis but also often for small-scale purification. Currently, there is limited understanding of the quantitative modeling of
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9464d3df2414d09046afca9535ed6952
http://urn.kb.se/resolve?urn=urn:nbn:se:kau:diva-93758
http://urn.kb.se/resolve?urn=urn:nbn:se:kau:diva-93758
Tributylamine (TBuA) and triethylamine (TEtA) are the most commonly used ion pair reagents in ion pair chromatography especially for the analysis of oligonucleotides. In order to improve the understanding of the retention and separation mechanism of
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::966a216cb1ee0288a0590d5f6bab69a0
http://urn.kb.se/resolve?urn=urn:nbn:se:kau:diva-92771
http://urn.kb.se/resolve?urn=urn:nbn:se:kau:diva-92771
Publikováno v:
Journal of chromatography. A. 1682
There is strong renewed interest in ion-pair chromatography (IPC) because of its great importance for separating new-generation biosimilar pharmaceuticals such as oligonucleotides. Due to the complexity of the IPC process, its mathematical modeling i
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5056e578e68f86676868a565396363d5
https://pubmed.ncbi.nlm.nih.gov/36122513
https://pubmed.ncbi.nlm.nih.gov/36122513
Publikováno v:
Analytical Chemistry
The traditional approach for analyzing interaction data from biosensors instruments is based on the simplified assumption that also larger biomolecules interactions are homogeneous. It was recently reported that the human receptor angiotensin-convert
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b229548c7f14742f3b1da471753734f9
https://doi.org/10.1021/acs.analchem.0c02475
https://doi.org/10.1021/acs.analchem.0c02475
Support vector regression models are created and used to predict the retention times of oligonucleotides separated using gradient ion-pair chromatography with high accuracy. The experimental dataset consisted of fully phosphorothioated oligonucleotid
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8f1211620be99bbc272a049378bb0526
http://urn.kb.se/resolve?urn=urn:nbn:se:kau:diva-89822
http://urn.kb.se/resolve?urn=urn:nbn:se:kau:diva-89822
Autor:
Jörgen Samuelsson, Linda Thunberg, Fredrik Limé, Eivor Örnskov, Torgny Fornstedt, Martin Enmark, Joakim Bagge, Hanna Leek
Publikováno v:
Analytical and Bioanalytical Chemistry
Oligonucleotide drugs represent an emerging area in the pharmaceutical industry. Solid-phase synthesis generates many structurally closely related impurities, making efficient separation systems for purification and analysis a key challenge during ph
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::faab313cb73d48cdc0fb8b4c7726ae96
https://doi.org/10.1007/s00216-019-02236-9
https://doi.org/10.1007/s00216-019-02236-9
Publikováno v:
Microchemical Journal. 145:927-935
Thermal imaging was used to visualise axial temperature gradients in chromatography columns running solvents of water, methanol and acetonitrile at various compositions. The non-monotonic relationship between solvent composition and viscosity enabled
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::976d342dc9212436a0f87a2bc556c535
https://doi.org/10.1016/j.microc.2018.11.037
https://doi.org/10.1016/j.microc.2018.11.037
Publikováno v:
Journal of chromatography. A. 1656
The effective separation of many solutes, including pharmaceuticals, can be performed using an ion-pair reagent (IPR) in the mobile phase. However, chromatographic separation and mathematical modelling are a challenge in ionpair chromatography (IPC),
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1e5a02f008904f866dfb3511c3ca2f79
https://pubmed.ncbi.nlm.nih.gov/34537662
https://pubmed.ncbi.nlm.nih.gov/34537662
Here, overloaded concentration profiles were predicted in supercritical fluid chromatography using a combined two-dimensional heat and mass transfer model. The heat balance equation provided the temperature and pressure profiles inside the column. Fr
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a4996e7a5b4fd8578af7de7596a1ad2d
http://urn.kb.se/resolve?urn=urn:nbn:se:kau:diva-83351
http://urn.kb.se/resolve?urn=urn:nbn:se:kau:diva-83351
Autor:
Said Harun, Eivor Örnskov, Anders Dahlén, Linda Thunberg, Torgny Fornstedt, Martin Enmark, Jörgen Samuelsson
A B S T R A C T Here it was investigated how oligonucleotide retention and selectivity factors are affected by electrostatic and non-electrostatic interactions in ion pair chromatography. A framework was derived describing how selectivity depends on
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4a1ff576e3d66a4394585a43ac707378
http://urn.kb.se/resolve?urn=urn:nbn:se:kau:diva-85296
http://urn.kb.se/resolve?urn=urn:nbn:se:kau:diva-85296