Zobrazeno 1 - 10
of 313
pro vyhledávání: '"J, Bragado"'
Publikováno v:
Biology, Vol 11, Iss 5, p 659 (2022)
Infertility affects approximately 15% of couples worldwide of childbearing age, and in many cases the etiology of male infertility is unknown. The current standard evaluation of semen is insufficient to establish an accurate diagnosis. Proteomics tec
Externí odkaz:
https://doaj.org/article/e8bca9a725c44343887fde238aa6c814
Autor:
Violeta Calle-Guisado, Ana Hurtado de Llera, David Martin-Hidalgo, Jose Mijares, Maria C Gil, Ignacio S Alvarez, Maria J Bragado, Luis J Garcia-Marin
Publikováno v:
Asian Journal of Andrology, Vol 19, Iss 6, Pp 707-714 (2017)
AMP-activated kinase (AMPK), a protein that regulates energy balance and metabolism, has recently been identified in boar spermatozoa where regulates key functional sperm processes essential for fertilization. This work′s aims are AMPK identificati
Externí odkaz:
https://doaj.org/article/49a3f0e2838d4edbb849da73220e6243
Autor:
Pablo Fernández-Hernández, María Jesús Sánchez-Calabuig, Luis Jesús García-Marín, María J. Bragado, Alfonso Gutiérrez-Adán, Óscar Millet, Chiara Bruzzone, Lauro González-Fernández, Beatriz Macías-García
Publikováno v:
Animals, Vol 10, Iss 5, p 883 (2020)
Production of equine embryos in vitro is currently a commercial technique and a reliable way of obtaining offspring. In order to produce those embryos, immature oocytes are retrieved from postmortem ovaries or live mares by ovum pick-up (OPU), mature
Externí odkaz:
https://doaj.org/article/2d29c0c548e9497eb78c45bb7fc59b01
Autor:
Lauro González-Fernández, Andrea Gomez-Candelo, Soraya Solar-Malaga, Maria J. Bragado, Mercedes Torres-Badia, David Martin-Hidalgo, Ana Hurtado de Llera, Luis J. Garcia-Marin
Publikováno v:
Theriogenology. 172:55-66
The use of worldwide glyphosate-based herbicide Roundup® is growing and to date its effects on mammalian spermatozoa are controversial. This study aims to investigate the functional impact of in vitro exposure of pig spermatozoa to low concentration
Autor:
María Jesús Sánchez-Calabuig, Maria J. Bragado, Alfonso Gutiérrez-Adán, Luis J. Garcia-Marin, Lauro González-Fernández, Pablo Fernández-Hernández, Chiara Bruzzone, Beatriz Macías-García, Oscar Millet
Publikováno v:
Animals : an Open Access Journal from MDPI
Animals, Vol 10, Iss 883, p 883 (2020)
Animals
Volume 10
Issue 5
Animals, Vol 10, Iss 883, p 883 (2020)
Animals
Volume 10
Issue 5
Production of equine embryos in vitro is currently a commercial technique and a reliable way of obtaining offspring. In order to produce those embryos, immature oocytes are retrieved from postmortem ovaries or live mares by ovum pick-up (OPU), mature
Autor:
Luis J. Garcia-Marin, Lauro González-Fernández, Jose A Céspedes, Nicolás Garrido, Maria J. Bragado, Rebeca Serrano
Publikováno v:
International Journal of Molecular Sciences, Vol 21, Iss 4, p 1208 (2020)
International Journal of Molecular Sciences
Volume 21
Issue 4
International Journal of Molecular Sciences
Volume 21
Issue 4
Excessive levels of reactive nitrogen species (RNS) produce nitrosative stress. Among RNS is peroxynitrite, a highly reactive free radical generated when nitric oxide reacts with superoxide anion. Peroxynitrite effects have been mainly studied in som
Autor:
Ana Hurtado de Llera, David Martin-Hidalgo, María C Gil, Luis J Garcia-Marin, María J Bragado
Publikováno v:
PLoS ONE, Vol 7, Iss 6, p e38840 (2012)
The main functions of spermatozoa required for fertilization are dependent on the energy status and metabolism. AMP-activated kinase, AMPK, acts a sensor and regulator of cell metabolism. As AMPK studies have been focused on somatic cells, our aim wa
Externí odkaz:
https://doaj.org/article/5700c31b657442548d14cb3221ae6bae
Publikováno v:
Animal Reproduction Science. 196:176-183
Frozen-thawed boar sperm have less motility and fertility capacity in comparison to fresh sperm. Glycogen Synthase Kinase 3 (GSK3) contributes to sperm motility in fresh semen. In addition, GSK3 inhibition in boar spermatozoa in fresh semen improves
Autor:
Lauro González-Fernández, Violeta Calle-Guisado, Luis J. Garcia-Marin, Maria J. Bragado, A. Hurtado de Llera
Publikováno v:
Andrology. 5:1131-1140
AMP-activated kinase (AMPK) plays a key function in maintaining cellular energy homeostasis. We recently identified and localized AMPK protein in human spermatozoa and showed that inhibition of AMPK activity significantly modified human sperm motilit
Autor:
Violeta Calle-Guisado, María Jesús Sánchez-Calabuig, Pablo Fernández-Hernández, Beatriz Macías-García, Alfonso Gutiérrez-Adán, Lauro González-Fernández, Maria J. Bragado, Luis J. Garcia-Marin
Publikováno v:
Theriogenology. 143
A repeatable protocol for equine in vitro fertilization (IVF) has remained elusive. This is likely, in part, due to suboptimal composition of capacitation or IVF media that are currently in use. Hence, we aimed to analyse the metabolome of equine ovi