Zobrazeno 1 - 10
of 10
pro vyhledávání: '"Ivan V. Polozov"'
Publikováno v:
Biochemistry. 45:15583-15590
We report on a novel reconstitution method for G-protein-coupled receptors (GPCRs) that yields detergent-free, single, tubular membranes in porous anodic aluminum oxide (AAO) filters at concentrations sufficient for structural studies by solid-state
Publikováno v:
Langmuir. 20:7711-7719
Lipid bilayers were deposited inside the 0.2 μm pores of anodic aluminum oxide (AAO) filters by extrusion of multilamellar liposomes and their properties studied by 2 H, 3 1 P, and 1 H solid-state NMR. Only the first bilayer adhered strongly to the
Publikováno v:
Biophysical Journal. 86:2910-2922
We use (2)H-NMR, (1)H-MAS NMR, and fluorescence microscopy to detect immiscibility in three particular phospholipid ratios mixed with 30% cholesterol: 2:1 DOPC/DPPC, 1:1 DOPC/DPPC, and 1:2 DOPC/DPPC. Large-scale (160 nm) phase separation into liquid-
Autor:
Klaus Gawrisch, Ivan V. Polozov, Nadukkudy V. Eldho, Scott E. Feller, Stephanie Tristram-Nagle
Publikováno v:
Journal of the American Chemical Society. 125:6409-6421
Insufficient supply to the developing brain of docosahexaenoic acid (22:6n3, DHA), or its omega-3 fatty acid precursors, results in replacement of DHA with docosapentaenoic acid (22:5n6, DPA), an omega-6 fatty acid that is lacking a double bond near
Publikováno v:
Chemistry and Physics of Lipids. 116:135-151
Nuclear magnetic resonance (NMR) studies on biomembranes have benefited greatly from introduction of magic angle spinning (MAS) NMR techniques. Improvements in MAS probe technology, combined with the higher magnetic field strength of modern instrumen
A method to isolate large quantities of directly accessible plasma membrane from attached cells is presented. The method is based upon the adhesion of cells to an adsorbed layer of polylysine on glass plates, followed by hypotonic lysis with ice-cold
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f7f721209ee4e9800bfe987b05113c21
https://europepmc.org/articles/PMC3541009/
https://europepmc.org/articles/PMC3541009/
Autor:
Ivan V. Polozov, Klaus Gawrisch
Publikováno v:
Methods in Molecular Biology ISBN: 9781588297297
Methods for detection of lateral domains by solid-state 2H nuclear magnetic resonance (NMR) and 1H magic angle spinning (MAS)-NMR in model- and biomembranes are presented. 2H NMR has been used for decades to distinguish between liquid-ordered and sol
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::be0f1553f96fde6a9bb1910f38149798
https://doi.org/10.1007/978-1-59745-513-8_9
https://doi.org/10.1007/978-1-59745-513-8_9
Autor:
Euijung Jo, Joan M. Boggs, Jack Blazyk, Raquel F. Epand, Gattadahalli M. Anantharamaiah, Ivan V. Polozov, Richard M. Epand
Publikováno v:
Biochimica et biophysica acta. 1511(1)
Magainins and other antimicrobial peptides increase ion flux across the membrane. They may do this by forming some type of pore or by perturbing lipid organization due to peptide lying on the bilayer surface. In order to determine if magainins pertur
Autor:
Ivan V. Polozov, Klaus Gawrisch
Publikováno v:
Biophysical Journal. (6):2051-2061
We investigated if magic angle spinning (MAS) 1 H NMR can be used as a tool for detection of liquid-ordered domains (rafts) in membranes. In experiments with the lipids SOPC, DOPC, DPPC, and cholesterol we demonstrated that 1 H MAS NMR spectra of liq
Autor:
Ivan V. Polozov, Klaus Gawrisch
Publikováno v:
Biophysical Journal. (3):1741-1751
We studied domain formation in mixtures of the monounsaturated lipids SOPC and POPE as a function of temperature and composition by NMR. Magic angle spinning at kHz frequencies restored resolution of (1)H NMR lipid resonances in the fluid phase, wher