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of 19
pro vyhledávání: '"Ivan L. Budyak"'
Autor:
Junyan Yang, Raluca Ostafe, Christopher J. Welch, Brandy Verhalen, Ivan L. Budyak, Merlin L. Bruening
Publikováno v:
Analytical Chemistry.
Publikováno v:
Molecular pharmaceutics. 19(11)
Static light scattering (SLS) was used to characterize five monoclonal antibodies (MAbs) as a function of total ionic strength (TIS) at pH values between 5.5 and 7.0. Second osmotic virial coefficient (
Autor:
George M. Bou-Assaf, Ivan L. Budyak, Michael Brenowitz, Eric S. Day, David Hayes, John Hill, Ranajoy Majumdar, Paola Ringhieri, Peter Schuck, Jasper C. Lin
Publikováno v:
J Pharm Sci
Analytical ultracentrifugation (AUC) is a critical analytical tool supporting the development and manufacture of protein therapeutics. AUC is routinely used as an assay orthogonal to size exclusion chromatography for aggregate quantitation. This arti
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4e43b967f463b00c953dcfcd1b5533d9
https://europepmc.org/articles/PMC9232890/
https://europepmc.org/articles/PMC9232890/
Publikováno v:
Pharmaceutical research. 38(11)
Protein solubility is an important attribute of pharmaceutical monoclonal antibody (MAb) formulations, particularly at high MAb concentrations. PEG-induced protein precipitation has been routinely used to assess protein solubility. To provide insight
Publikováno v:
Analytical Biochemistry. 531:16-19
Appropriate characterization of soluble aggregates is an important aspect of biologics development and manufacturing, and sedimentation velocity analytical ultracentrifugation (SV-AUC) is often used an orthogonal technique to size-exclusion chromatog
Publikováno v:
Analytical Biochemistry. 605:113837
Sedimentation velocity analytical ultracentrifugation (SV-AUC) is routinely used for quantitation of soluble aggregates as an orthogonal technique to size-exclusion chromatography (SEC). SV-AUC presents many advantages over the SEC, yet lower precisi
Publikováno v:
Analytical Biochemistry. 513:36-38
Throughout the course of drug development there are many instances in which a variability assessment within a set of analytical data is required, which may be challenging for techniques that produce two-dimensional data. This note describes an interv
Publikováno v:
Biochemistry. 52:8843-8854
Susceptibility to aggregation is general to proteins because of the potential for intermolecular interactions between hydrophobic stretches in their amino acid sequences. Protein aggregation has been implicated in several catastrophic diseases, yet w
Publikováno v:
Journal of Molecular Biology. 425:3522-3535
Aromatic–aromatic interactions have long been believed to play key roles in protein structure, folding, and binding functions. However, we still lack full understanding of the contributions of aromatic–aromatic interactions to protein stability a
Autor:
Young Jun Kim, Georg Büldt, Torben Hoomann, Jörg Labahn, Ivan L. Budyak, Ionela Radu, Joachim Heberle, Martin Engelhard, Ramona Schlesinger
Publikováno v:
Biophysical Chemistry. 150:23-28
Sensory rhodopsin I (SRI) from Halobacterium salinarum mediates both positive and negative phototaxis in a light-dependent manner. SRI photoactivation elicits extensive structural changes which are transmitted to the cognate transducer (HtrI). The at