Zobrazeno 1 - 10
of 19
pro vyhledávání: '"Ivan L, Budyak"'
Autor:
Junyan Yang, Raluca Ostafe, Christopher J. Welch, Brandy Verhalen, Ivan L. Budyak, Merlin L. Bruening
Publikováno v:
Analytical Chemistry.
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b35130b5f32b7c5958a83811d0150c80
https://doi.org/10.1021/acs.analchem.3c00531
https://doi.org/10.1021/acs.analchem.3c00531
Publikováno v:
Molecular pharmaceutics. 19(11)
Static light scattering (SLS) was used to characterize five monoclonal antibodies (MAbs) as a function of total ionic strength (TIS) at pH values between 5.5 and 7.0. Second osmotic virial coefficient (
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::d99029e59a76ae1ab697937b2ffa45a0
https://pubmed.ncbi.nlm.nih.gov/36194430
https://pubmed.ncbi.nlm.nih.gov/36194430
Autor:
George M. Bou-Assaf, Ivan L. Budyak, Michael Brenowitz, Eric S. Day, David Hayes, John Hill, Ranajoy Majumdar, Paola Ringhieri, Peter Schuck, Jasper C. Lin
Publikováno v:
J Pharm Sci
Analytical ultracentrifugation (AUC) is a critical analytical tool supporting the development and manufacture of protein therapeutics. AUC is routinely used as an assay orthogonal to size exclusion chromatography for aggregate quantitation. This arti
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4e43b967f463b00c953dcfcd1b5533d9
https://europepmc.org/articles/PMC9232890/
https://europepmc.org/articles/PMC9232890/
Publikováno v:
Pharmaceutical research. 38(11)
Protein solubility is an important attribute of pharmaceutical monoclonal antibody (MAb) formulations, particularly at high MAb concentrations. PEG-induced protein precipitation has been routinely used to assess protein solubility. To provide insight
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6cab516c6fdd10fe632750aa790014c4
https://pubmed.ncbi.nlm.nih.gov/34647231
https://pubmed.ncbi.nlm.nih.gov/34647231
Publikováno v:
Analytical Biochemistry. 531:16-19
Appropriate characterization of soluble aggregates is an important aspect of biologics development and manufacturing, and sedimentation velocity analytical ultracentrifugation (SV-AUC) is often used an orthogonal technique to size-exclusion chromatog
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c1c5d9ffcad1977d5afc9bbfd2405080
https://doi.org/10.1016/j.ab.2017.05.018
https://doi.org/10.1016/j.ab.2017.05.018
Publikováno v:
Analytical Biochemistry. 605:113837
Sedimentation velocity analytical ultracentrifugation (SV-AUC) is routinely used for quantitation of soluble aggregates as an orthogonal technique to size-exclusion chromatography (SEC). SV-AUC presents many advantages over the SEC, yet lower precisi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::0153852bd9ccc245f4316c95c3b2123b
https://doi.org/10.1016/j.ab.2020.113837
https://doi.org/10.1016/j.ab.2020.113837
Publikováno v:
Analytical Biochemistry. 513:36-38
Throughout the course of drug development there are many instances in which a variability assessment within a set of analytical data is required, which may be challenging for techniques that produce two-dimensional data. This note describes an interv
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d6a6caf1f5762d85507c9ff42bf2f27f
https://doi.org/10.1016/j.ab.2016.08.021
https://doi.org/10.1016/j.ab.2016.08.021
Publikováno v:
Biochemistry. 52:8843-8854
Susceptibility to aggregation is general to proteins because of the potential for intermolecular interactions between hydrophobic stretches in their amino acid sequences. Protein aggregation has been implicated in several catastrophic diseases, yet w
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a4d11bc40747d4ddf3a905c4f0a001b4
https://doi.org/10.1021/bi4013462
https://doi.org/10.1021/bi4013462
Publikováno v:
Journal of Molecular Biology. 425:3522-3535
Aromatic–aromatic interactions have long been believed to play key roles in protein structure, folding, and binding functions. However, we still lack full understanding of the contributions of aromatic–aromatic interactions to protein stability a
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6fd840adbf1f7c553671b74ec4a41aa3
https://doi.org/10.1016/j.jmb.2013.06.030
https://doi.org/10.1016/j.jmb.2013.06.030
Autor:
Young Jun Kim, Georg Büldt, Torben Hoomann, Jörg Labahn, Ivan L. Budyak, Ionela Radu, Joachim Heberle, Martin Engelhard, Ramona Schlesinger
Publikováno v:
Biophysical Chemistry. 150:23-28
Sensory rhodopsin I (SRI) from Halobacterium salinarum mediates both positive and negative phototaxis in a light-dependent manner. SRI photoactivation elicits extensive structural changes which are transmitted to the cognate transducer (HtrI). The at
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fcac7f6a60d06a44e9eb609a57c7cf82
https://doi.org/10.1016/j.bpc.2010.02.017
https://doi.org/10.1016/j.bpc.2010.02.017