Sequence-specific stalling of DNA polymerase gamma and the effects of mutations causing progressive ophthalmoplegia

Autor: Neli Atanassova, Sjoerd Wanrooij, Nils-Göran Larsson, Bertil Macao, Steffi Goffart, Johannes N. Spelbrink, Géraldine Farge, Maria Falkenberg, Javier Miralles Fusté, Stefan Bäckström, Ivan Khvorostov

Publikováno v: Human Molecular Genetics, 20, 1212-23
Human Molecular Genetics, 20, 6, pp. 1212-23
Atanassova, N, Fuste, J M, Wanrooij, S, Macao, B, Goffart, S, Backstrom, S, Farge, G A, Khvorostov, I, Larsson, N G, Spelbrink, J N & Falkenberg, M 2011, ' Sequence-specific stalling of DNA polymerase gamma and the effects of mutations causing progressive ophthalmoplegia ', Human Molecular Genetics, vol. 20, no. 6, pp. 1212-1223 . https://doi.org/10.1093/hmg/ddq565
Human Molecular Genetics, 20(6), 1212-1223. Oxford University Press

A large number of mutations in the gene encoding the catalytic subunit of mitochondrial DNA polymerase γ (POLγA) cause human disease. The Y955C mutation is common and leads to a dominant disease with progressive external ophthalmoplegia and other s

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fce8918e04644eaa466769a77ad63c5e
https://doi.org/10.1093/hmg/ddq565

Somatic mtDNA mutations cause aging phenotypes without affecting reactive oxygen species production

Autor: Rolf Wibom, Anna Wredenberg, Johannes N. Spelbrink, Eric Dufour, Aleksandra Trifunovic, Howard T. Jacobs, Anna Hansson, Anja T. Rovio, Nils-Göran Larsson, Ivan Khvorostov

Publikováno v: Proceedings of the National Academy of Sciences. 102:17993-17998

The mitochondrial theory of aging proposes that reactive oxygen species (ROS) generated inside the cell will lead, with time, to increasing amounts of oxidative damage to various cell components. The main site for ROS production is the respiratory ch

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::31a4d1713a77dad3e39c0a5b0fb09307
https://doi.org/10.1073/pnas.0508886102

UCP2 regulates energy metabolism and differentiation potential of human pluripotent stem cells

Autor: Jin, Zhang, Ivan, Khvorostov, Jason S, Hong, Yavuz, Oktay, Laurent, Vergnes, Esther, Nuebel, Paulin N, Wahjudi, Kiyoko, Setoguchi, Geng, Wang, Anna, Do, Hea-Jin, Jung, J Michael, McCaffery, Irwin J, Kurland, Karen, Reue, Wai-Nang P, Lee, Carla M, Koehler, Michael A, Teitell

Publikováno v: The EMBO journal. 30(24)

It has been assumed, based largely on morphologic evidence, that human pluripotent stem cells (hPSCs) contain underdeveloped, bioenergetically inactive mitochondria. In contrast, differentiated cells harbour a branched mitochondrial network with oxid

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::595f32d0d73368cd9873a647401c889e
https://pubmed.ncbi.nlm.nih.gov/27084758

From MEFs to matrigel I: passaging hESCs in the presence of MEFs

Autor: Jin Zhang, Ivan Khvorostov, Michael A. Teitell

Publikováno v: Journal of visualized experiments : JoVE. (16)

This video demonstrates how to grow human embryonic stem cells (hESCs) on mouse embryonic fibroblast (MEF) feeder cells.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::74fc1251dcd4c838b7f0e78dafd45acb
https://pubmed.ncbi.nlm.nih.gov/19066554

From MEFs to Matrigel 2: Splitting hESCs from MEFs onto Matrigel

Autor: Ivan Khvorostov, Michael A. Teitell, Jin Zhang

This video demonstrates how to grow human embryonic stem cells (hESCs) on mouse embryonic fibroblast (MEF) feeder cells, how to passage hESCs from MEF plates to feeder cell-free Matrigel plates.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c89b8abfe3408a0fce728853dd184f4e
https://europepmc.org/articles/PMC2583032/