Human cellular model systems of β-thalassemia enable in-depth analysis of disease phenotype

Autor: Deborah E. Daniels, Ivan Ferrer-Vicens, Joseph Hawksworth, Tatyana N. Andrienko, Elizabeth M. Finnie, Natalie S. Bretherton, Daniel C. J. Ferguson, A. Sofia. F. Oliveira, Jenn-Yeu A. Szeto, Marieangela C. Wilson, John N. Brewin, Jan Frayne

Publikováno v: Nature Communications, Vol 14, Iss 1, Pp 1-14 (2023)

Abstract β-thalassemia is a prevalent genetic disorder causing severe anemia due to defective erythropoiesis, with few treatment options. Studying the underlying molecular defects is impeded by paucity of suitable patient material. In this study we

Externí odkaz: https://doaj.org/article/9939683767224f40b2a842f9297fb596

Reproducible immortalization of erythroblasts from multiple stem cell sources provides approach for sustainable RBC therapeutics

Autor: Deborah E. Daniels, Daniel C.J. Ferguson, Rebecca E. Griffiths, Kongtana Trakarnsanga, Nicola Cogan, Katherine A. MacInnes, Kathryn E. Mordue, Tatyana Andrienko, Ivan Ferrer-Vicens, Daniel Ramos Jiménez, Phillip A. Lewis, Marieangela C. Wilson, Maurice A. Canham, Ryo Kurita, Yukio Nakamura, David J. Anstee, Jan Frayne

Publikováno v: Molecular Therapy: Methods & Clinical Development, Vol 22, Iss , Pp 26-39 (2021)

Developing robust methodology for the sustainable production of red blood cells in vitro is essential for providing an alternative source of clinical-quality blood, particularly for individuals with rare blood group phenotypes. Immortalized erythroid

Externí odkaz: https://doaj.org/article/1be556ed511b484ca9104a2782e6751a

Comparing the two leading erythroid lines BEL-A and HUDEP-2

Autor: Deborah E. Daniels, Damien J. Downes, Ivan Ferrer-Vicens, Daniel C. J. Ferguson, Belinda K. Singleton, Marieangela C. Wilson, Kongtana Trakarnsanga, Ryo Kurita, Yukio Nakamura, David J. Anstee, Jan Frayne

Publikováno v: Haematologica, Vol 105, Iss 8 (2020)

Externí odkaz: https://doaj.org/article/45b08dbb99fb489a9a8511a89e4e0a31

Reproducible immortalization of erythroblasts from multiple stem cell sources provides approach for sustainable RBC therapeutics

Autor: David J. Anstee, Phillip A. Lewis, Daniel Ramos Jiménez, Tatyana N Andrienko, Kathryn E. Mordue, Deborah E. Daniels, Marieangela C. Wilson, Ivan Ferrer-Vicens, Yukio Nakamura, Rebecca E. Griffiths, Daniel C. J. Ferguson, Ryo Kurita, Maurice A. Canham, Kongtana Trakarnsanga, Nicola Cogan, Jan Frayne, Katherine A. MacInnes

Publikováno v: Molecular Therapy: Methods & Clinical Development, Vol 22, Iss, Pp 26-39 (2021)
Daniels, D, Ferguson, D C J, Trakarnsanga, T, Cogan, N M, MacInnes, K, Andrienko, T N, Ferrer Vicens, I, Wilson, M C, Anstee, D J & Frayne, J 2021, ' Reproducible immortalization of erythroblasts from multiple stem cell sources provides approach for sustainable RBC therapeutics ', Molecular Therapy-Methods and Clinical Development, vol. 22, pp. 26-39 . https://doi.org/10.1016/j.omtm.2021.06.002
Molecular Therapy. Methods & Clinical Development

Developing robust methodology for the sustainable production of red blood cells in vitro is essential for providing an alternative source of clinical-quality blood, particularly for individuals with rare blood group phenotypes. Immortalized erythroid

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::afecb6f33a3bd09a2f78b76a3eade7c7
https://doi.org/10.1016/j.omtm.2021.06.002

Characterization and evolutionary origin of novel C2H2 zinc finger protein (ZNF648) required for both erythroid and megakaryocyte differentiation in humans

Autor: Deborah K. Shoemark, Daniel C. J. Ferguson, Ivan Ferrer-Vicens, Marieangela C. Wilson, Kongtana Trakarnsanga, Sarah Cooke, Marjolein Meinders, Deborah E. Daniels, Katherine A. MacInnes, Juraidah Haji Mokim, Jan Frayne, Tom A. Williams, Chartsiam Tipgomut, Edmund R. R. Moody, Belinda K. Singleton

Publikováno v: Haematologica. 106:2859-2873

Human ZNF648 is a novel poly C-terminal C2H2 zinc finger (ZnF) protein identified amongst the most dysregulated proteins in erythroid cells differentiated from induced pluripotent stem cells. Its nuclear localization and structure indicate it is like

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::216591e5722a228ade2e1527a0877313
https://doi.org/10.3324/haematol.2020.256347