Zobrazeno 1 - 10
of 91
pro vyhledávání: '"Ivan A. Sakharov"'
Publikováno v:
Biosensors, Vol 12, Iss 8, p 570 (2022)
In the present work, we describe the development of a chemiluminescent enzyme-linked oligonucleotide assay coupled with mismatched catalytic hairpin assembly (mCHA) amplification for the quantitative determination of microRNA-155. To improve its sens
Externí odkaz:
https://doaj.org/article/31981fc30a224ee9b3ecd8072e56403d
Autor:
Oleg L, Bodulev, Ivan Yu, Sakharov
Publikováno v:
Biochemistry (Moscow). 87:425-442
The review discusses modern methods for the quantitative and semi-quantitative analysis of miRNAs, which are small non-coding RNAs affecting numerous biological processes such as development, differentiation, metabolism, and immune response. miRNAs a
Autor:
Anton M. Solovjev, Ivan I. Galkin, Alexey V. Medved'ko, Olga Yu. Pletjushkina, Shulin Zhao, Ivan Yu. Sakharov
Publikováno v:
The Analyst. 147:4293-4300
Heterogeneous and homogeneous-heterogeneous assays for the quantitation of hsa-miR-141-3p (miRNA-141) were constructed. Both microplate assays were based on the use of the isothermal circular strand-displacement polymerization reaction (ICSDPR), whic
Publikováno v:
Analytical and Bioanalytical Chemistry. 412:5105-5111
Nowadays, considerable efforts are focused on advancing DNA detection methods, which are extremely important in clinical diagnostics, pathogen determination, gene therapy, and forensic analysis. A one-pot sensitive microplate-based chemiluminescent a
Publikováno v:
Talanta. 253:124011
Publikováno v:
Revista Colombiana de Química, Vol 28, Iss 1, Pp 97-106 (2010)
Se determinó la actividad de la peroxidasa (EC 1.11.1.7) en 7 frutos, 13 raíces y 31 hojas de plantas tropicales que crecen en Colombia. Se encontró que la actividad de la peroxidasa varía ampliamente de una especie a otra. Para el fruto de balaz
Externí odkaz:
https://doaj.org/article/a1f6e9daa7764a8685c3722682057a53
Autor:
Irina V. Safenkova, Konstantin M. Burkin, Oleg L. Bodulev, Shyatesa C. Razo, Aleksandr V. Ivanov, Anatoly V. Zherdev, Boris B. Dzantiev, Ivan Yu Sakharov
Publikováno v:
Talanta. 247:123535
Magnetic beads (MBs) are often considered as an effective carrier in heterogeneous assays due to the simplicity of separation and washing, and the ability to increase and control the surface area. However, the effect of the MBs surface on the analyti
Publikováno v:
Analytical chemistry. 93(26)
The absolute quantification of miRNAs in a single cell allows to better understand the heterogeneity of cells and the relationship between miRNAs and diseases. However, seldom methods for miRNA quantification in a single cell have been reported becau
Publikováno v:
Analytical Letters. 53:140-151
A microplate-based sandwich assay for the determination of α-human thrombin (HTb) was developed. Fluorescein-modified 29-mer thrombin binding aptamer (FAM-TBA29) and biotinylated 15-mer thrombin bi...
Autor:
Anna Yu Kolosova, Ivan Yu. Sakharov
Publikováno v:
Analytical Letters. 52:1352-1362
Significant research efforts are currently focused on advancing DNA detection methodology. Various nanoparticles (NPs), which are currently the most widely used solid-phase carriers for nucleic acid assays, have a number of essential drawbacks. Micro