Soft-sensors application for automated feeding control in high-throughput mammalian cell cultures

Autor: Iván Martínez‐Monge, Carlos Martínez, Marianne Decker, Isuru A. Udugama, Igor Marín de Mas, Krist V. Gernaey, Lars K. Nielsen

Publikováno v: Martínez-Monge, I, Martínez, C, Decker, M, Udugama, I A, Marín de Mas, I, Gernaey, K V & Nielsen, L K 2022, ' Soft-sensors application for automated feeding control in high-throughput mammalian cell cultures ', Biotechnology and Bioengineering, vol. 119, no. 4, pp. 1077-1090 . https://doi.org/10.1002/bit.28032

The ever-increasing demand for biopharmaceuticals has created the need for improving the overall productivity of culture processes. One such operational concept that is considered is fed-batch operations as opposed to batch operations. However, optim

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5310bbdb033bc57b10c8a40b48ac1931
https://orbit.dtu.dk/en/publications/3b1685d4-5ab9-4737-b414-d28fecf9b9eb

The Effect of the Expression of the Antiapoptotic BHRF1 Gene on the Metabolic Behavior of a Hybridoma Cell Line

Autor: Jordi J. Cairó, Jordi Prat, Carlos J. Paredes, P. Comas, Antoni Casablancas, David Catalán-Tatjer, Iván Martínez-Monge, Martí Lecina

Publikováno v: Martínez-Monge, I, Comas, P, Catalán-Tatjer, D, Prat, J, Casablancas, A, Paredes, C, Lecina, M & Cairó, J J 2021, ' The Effect of the Expression of the Antiapoptotic BHRF1 Gene on the Metabolic Behavior of a Hybridoma Cell Line ', Applied Sciences, vol. 11, no. 14, 6258 . https://doi.org/10.3390/app11146258
Applied Sciences, Vol 11, Iss 6258, p 6258 (2021)
Applied Sciences
Volume 11
Issue 14

One of the most important limitations of mammalian cells-based bioprocesses, and particularly hybridoma cell lines, is the accelerated metabolism related to glucose and glutamine consumption. The high uptake rates of glucose and glutamine (i.e., the

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::d632ae8f68cde40a306599decd517418
https://orbit.dtu.dk/en/publications/9877a23e-a569-424b-9313-e61c2470184e

A new strategy for fed-batch process control of HEK293 cell cultures based on alkali buffer addition monitoring: comparison with O.U.R. dynamic method

Autor: Jordi J. Cairó, P. Comas, Martí Lecina, J. Triquell, Iván Martínez‐Monge, Antoni Casablancas

Publikováno v: Applied microbiology and biotechnology. 102(24)

The increasing demand for biopharmaceuticals produced in mammalian cells has driven the industry to enhance productivity of bioprocesses through different strategies. This is why fed-batch and perfusion cultures are considered more attractive choices

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8e83c1e75c24b4d1fc1dae1d2307e0e6
https://pubmed.ncbi.nlm.nih.gov/30288587