Zobrazeno 1 - 10
of 36
pro vyhledávání: '"Iván Coto Hernández"'
Publikováno v:
Scientific Reports, Vol 12, Iss 1, Pp 1-10 (2022)
Abstract Traditional histopathologic evaluation of peripheral nerve employs brightfield microscopy with diffraction limited resolution of ~ 250 nm. Though electron microscopy yields nanoscale resolution of the nervous system, sample preparation is co
Externí odkaz:
https://doaj.org/article/92c977153ac14415a9cad2c5aaaf9888
Publikováno v:
Scientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
Abstract Fluoro-Gold is a fluorescent neuronal tracer suitable for targeted deep imaging of the nervous system. Widefield fluorescence microscopy enables visualization of Fluoro-Gold, but lacks depth discrimination. Though scanning laser confocal mic
Externí odkaz:
https://doaj.org/article/1fc7e0ca3e394382b0ee2b08eabd655a
Publikováno v:
Journal of the Optical Society of America. A, Optics, image science, and vision. 39(4)
Spatial resolution of stimulated emission depletion (STED) microscopy varies with sample labeling techniques and microscope components, e.g., lasers, lenses, and photo-detectors. Fluctuations in the intensity of the depletion laser decrease achievabl
Publikováno v:
Methods and Applications in Fluorescence. 11:013001
Multiphoton microscopy (MPM) employs ultrafast infrared lasers for high-resolution deep three-dimensional imaging of live biological samples. The goal of this tutorial is to provide a practical guide to MPM imaging for novice microscopy developers an
Publikováno v:
Rishøj, L, Hernandez, I C, Ramachandran, S & Jowett, N 2022, ' Multiphoton microscopy for label-free multicolor imaging of peripheral nerve ', Journal of Biomedical Optics, vol. 27, no. 5, 056501 . https://doi.org/10.1117/1.JBO.27.5.056501
Significance: Means for quantitation of myelinated fibers in peripheral nerve may guide diagnosis and clinical decision making in management of peripheral nerve disorders. Multiphoton microscopy techniques such as the third-harmonic generation enable
Conventional histomorphometry of peripheral nerve entails lengthy chemical processing, ultrathin sectioning in resin, and imaging by light or electron microscopy. Multiphoton microscopy techniques exist enabling label-free and in vivo imaging of hist
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::4b49f7af3952a217a89dca85caa8c6ea
https://doi.org/10.1101/2021.09.30.462641
https://doi.org/10.1101/2021.09.30.462641
Autor:
Nate Jowett, Carrie Ng, Casey A. Maguire, Steven Minderler, Josette Nammour, Iván Coto Hernández, Matthew Q. Miller
Peripheral nerve injuries yield devastating consequences, and surgical repair outcomes remain suboptimal. Novel therapeutic strategies such as gene therapy could improve peripheral nerve regeneration. Though adeno-associated virus (AAV) vectors have
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::d0f6002306c94e034d553b9e896197a4
https://doi.org/10.21203/rs.3.rs-708393/v1
https://doi.org/10.21203/rs.3.rs-708393/v1
Publikováno v:
Plastic and Reconstructive Surgery. 143:771-778
BACKGROUND Donor nerve myelinated axon counts correlate with functional outcomes in reanimation procedures; however, there exists no reliable means for their intraoperative quantification. In this article, the authors report a novel protocol for rapi
Publikováno v:
Journal of Neuroscience Methods. 375:109598
Traditional histopathologic evaluation of peripheral nerve using brightfield microscopy is resource-intensive, necessitating complex sample preparation. Label-free imaging techniques paired with artificial intelligence-based image reconstruction and
Publikováno v:
Scientific Reports
Scientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
Scientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
Fluoro-Gold is a fluorescent neuronal tracer suitable for targeted deep imaging of the nervous system. Widefield fluorescence microscopy enables visualization of Fluoro-Gold, but lacks depth discrimination. Though scanning laser confocal microscopy y
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ff3cedb93c29ce66240bc1800ad4c1c2
https://doi.org/10.21203/rs.3.rs-200482/v1
https://doi.org/10.21203/rs.3.rs-200482/v1