A near-infrared fluorogenic dimer enables background-free imaging of endogenous GPCRs in living mice

Autor: David Brasse, Thierry Durroux, Dominique Bonnet, Lucie Esteoulle, Patrice Marchand, Iuliia A. Karpenko, Andrey S. Klymchenko, Stéphanie Riché, François Daubeuf, Mayeul Collot

Publikováno v: Chemical Science
Chemical Science, The Royal Society of Chemistry, 2020, 11 (26), pp.6824-6829. ⟨10.1039/d0sc01018a⟩
Chemical Science, 2020, 11 (26), pp.6824-6829. ⟨10.1039/d0sc01018a⟩

International audience; Fluorescent probes are commonly used in studying G protein-coupled receptors in living cells; however their application to the whole animal receptor imaging is still challenging. To address this problem, we report the design a

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::abccfc845429889f672add74f01419d4
https://doi.org/10.1039/d0sc01018a

Dual emissive analogue of deoxyuridine as a sensitive hydration-reporting probe for discriminating mismatched from matched DNA and DNA/DNA from DNA/RNA duplexes

Autor: Delphine Debayle, Nicolas P. F. Barthes, Dmytro Dziuba, Alexander P. Demchenko, Benoît Y. Michel, Krishna Gavvala, Anne Sophie Dabert-Gay, Iuliia A. Karpenko, Rachid Benhida, Dominique Bonhomme, Yves Mély, Alain Burger

Publikováno v: Journal of Materials Chemistry C. 4:3010-3017

Fluorescent nucleosides with high sensitivity to H-bonding are of great interest not only to investigate DNA hydration and DNA–protein interactions and dynamics, but also to develop screening assays. Here, we report on the incorporation of a dual e

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::c72c73474181edd56a67f3b947f4b55a
https://doi.org/10.1039/c5tc03427b

Selective Nonpeptidic Fluorescent Ligands for Oxytocin Receptor: Design, Synthesis, and Application to Time-Resolved FRET Binding Assay

Autor: Elsa Pflimlin, Thierry Durroux, Iuliia A. Karpenko, Thiéric Rodriguez, Jean-François Margathe, Elodie Dupuis, Marcel Hibert, Dominique Bonnet

Publikováno v: Journal of Medicinal Chemistry. 58:2547-2552

The design and the synthesis of the first high-affinity fluorescent ligands for oxytocin receptor (OTR) are described. These compounds enabled the development of a TR-FRET based assay for OTR, readily amenable to high throughput screening. The valida

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::816e50dbb2308aa241ce336db42b1028
https://doi.org/10.1021/jm501395b

Red Fluorescent Turn-On Ligands for Imaging and Quantifying G Protein-Coupled Receptors in Living Cells

Autor: Iuliia A. Karpenko, Christel Valencia, Dominique Bonnet, Bernard Mouillac, Yves Mély, Marcel Hibert, Rémy Kreder, Andrey S. Klymchenko, Christiane Mendre, Pascal Villa

Publikováno v: ChemBioChem. 15:359-363

Classical fluorescence-based approaches to monitor ligand-protein interactions are generally hampered by the background signal of unbound ligand, which must be removed by tedious washing steps. To overcome this major limitation, we report here the fi

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::952ba367224166cd1737db259d06df44
https://doi.org/10.1002/cbic.201300738

Synthesis of calixarene-based ketocyanine fluorophores

Autor: Svitlana V. Shishkina, Vasyl G. Pivovarenko, Aleksey B. Ryabitskii, Yuriy Matvieiev, Iuliia A. Karpenko, Vitaly I. Kalchenko, Andrii V. Kulinich, Oleg V. Shishkin

Publikováno v: Tetrahedron Letters. 52:3922-3925

Cup-shaped calix[4]arenes bearing one or two ketocyanine fluorophore fragments at the wide rim of the macrocycle are synthesized by condensation of formyl calix[4]arenes with arylmethylene(hetarylmethylene)cyclopentanones in the presence of the ionic

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::c9820b8ce5c327cbc9022d1fff381d11
https://doi.org/10.1016/j.tetlet.2011.05.094