Zobrazeno 1 - 10
of 12
pro vyhledávání: '"Iu G, Popov"'
Publikováno v:
Prikladnaia biokhimiia i mikrobiologiia. 45(1)
L-Phenylalanine ammonia-lyase (PAL, EC 4.3.1.5) of the Rhodotorula aurantiaca strain KM-1 deaminates L-phenylalanine according to the Michaelis-Menten kinetics with K(M) = 1.75 +/- 0.44 mM and V(max) = 3.01 +/- 0.43 units/mg. The enzyme is competitiv
Publikováno v:
Genetika. 37(6)
It is shown that bacterial genes for thermostable beta-glucanases are expressed retaining their activity and substrate specificity. The leader peptide of the carrot extensin exerts effective secretion of the bacterial enzymes into the intercellular s
Publikováno v:
Genetika. 32(2)
The modified hybrid beta-1,3-glucanase gene (glc) of Clostridium thermocellum was expressed in tobacco Nicotiana tabacum. The glc gene was cloned into two plasmids, pC27-glc and pC29-glc, in which its expression was controlled by the TR2' promoter of
Publikováno v:
Genetika. 32(2)
We constructed two vectors, pC27-glc and pC29-glc, that allow expression of the beta-1,3-glucanase gene (glc) in plant cells. The glc gene was previously cloned from anaerobic thermophilous bacterium Clostridium thermocellum. To increase the efficien
Publikováno v:
Gematologiia i transfuziologiia. 38(2)
In the study of L-glutaminic acid effects on human platelet functional activity it was established that 30-60-min treatment of platelet-rich plasma with glutamate in concentration 0.1-1.0 mM at 37 degrees C results in a marked platelet activation. Th
Autor:
A D, Scholl-Engberts, E N, Sokolova, Z A, Darieva, Iu G, Popov, T V, Pospelova, V S, Gurevich
Publikováno v:
Tsitologiia. 34(5)
In view of recent studies showing that cell proliferation of E1Aad5+c-Ha-ras-transformed fibroblasts cannot be regulated by growth factors and phorbol eaters in contrast to normal and E1Aad5-immortalized cell lines, the present work was undertaken to
Publikováno v:
Biokhimiia (Moscow, Russia). 56(9)
Solubilization of the total membrane fraction of human platelets in a 2% solution of sodium deoxycholate and subsequent affinity chromatography on glutamate agarose resulted in two protein fractions possessing a glutamate-binding activity. As can be
Publikováno v:
Gematologiia i transfuziologiia. 35(10)
Publikováno v:
Biokhimiia (Moscow, Russia). 55(5)
A simple and rapid procedure for isolation of the total platelet membrane fraction by chromatography on Sepharose CL 6B has been developed. This method allows a rapid (25-30 min) one-step separation of the membrane fraction on 26 x 150 mm columns wit
Publikováno v:
Klinicheskaia khirurgiia. (5)