Zobrazeno 1 - 6
of 6
pro vyhledávání: '"Itzcoatl A. Pla"'
Publikováno v:
Biotechnology Progress. 36
Glycoproteins could be highly sialylated, and controlling the sialic acid levels for some therapeutic proteins is critical to ensure product consistency and efficacy. N-acetylneuraminic acid (Neu5Ac, or NANA) and N-glycolylneuraminic acid (Neu5Gc, or
Autor:
Ting Shi, Scott A. Miller, Douglas G. Weaver, Michael Casati, Wei Ding, Donald W. Kessler, Charles Pathirana, Itzcoatl A. Pla, Nancy A. Mackin, Russell D. Martin, Nuria de Mas
Publikováno v:
PDA Journal of Pharmaceutical Science and Technology. 70:272-281
Air–liquid interface rings were observed on the side walls of stainless steel buffer vessels after certain downstream buffer preparations. Those rings were resistant to regular cleaning-in-place procedures but could be removed by manual means. To i
Autor:
Gerd Ritter, Todd Vannelli, Michael L. Shuler, Carl A. Batt, Leonardo M. Damasceno, Itzcoatl A. Pla
Publikováno v:
Biotechnology Progress. 22:881-888
Extracellular secretion of over 4 g x L(-1) of the A33 scFv antibody fragment was achieved in Pichia pastoris at the 10 L bioreactor scale using minimal medium and feedback control of the methanol concentration. Since methanol acts as both inducer an
Autor:
Itzcoatl A, Pla, Leonardo M, Damasceno, Todd, Vannelli, Gerd, Ritter, Carl A, Batt, Michael L, Shuler
Publikováno v:
Biotechnology progress. 22(3)
Extracellular secretion of over 4 g x L(-1) of the A33 scFv antibody fragment was achieved in Pichia pastoris at the 10 L bioreactor scale using minimal medium and feedback control of the methanol concentration. Since methanol acts as both inducer an
Autor:
Itzcoatl A. Pla, Leonardo M. Damasceno, Leonard Cohen, Gerd Ritter, Carl A. Batt, Hyun-Joo Chang, Lloyd J. Old
Publikováno v:
Protein expression and purification. 37(1)
The expression of a humanized single-chain variable domain fragment antibody (A33scFv) was optimized for Pichia pastoris with yields exceeding 4 g L(-1). A33scFv recognizes a cell surface glycoprotein (designated A33) expressed in colon cancer that s
Autor:
Itzcoatl A. Pla, Leonardo M. Damasceno, Todd Vannelli, Gerd Ritter, Carl A. Batt, Michael L. Shuler
Publikováno v:
Biotechnology Progress; Jun2006, Vol. 22 Issue 3, p881-888, 8p