A simple and cost-effective extraction for piscine environmental DNA metabarcoding using guanidine hydrochloride method.

Autor: Amin MHF; Department of Biology, Faculty of Sciences and Technology, Universitas Airlangga. Jl. Dr. Ir. H. Soekarno, Mulyorejo, Surabaya 60115, East Java, Indonesia.; Advanced Tropical Biodiversity, Genomics, and Conservation Research Group, Faculty of Science and Technology, Universitas Airlangga. Jl. Dr. Ir. H. Soekarno, Mulyorejo, Surabaya 60115, East Java, Indonesia., Kim HW; Department of Biology, Faculty of Sciences and Technology, Universitas Airlangga. Jl. Dr. Ir. H. Soekarno, Mulyorejo, Surabaya 60115, East Java, Indonesia.; Department of Marine Biology, Pukyong National University, Busan 48513, South Korea.; Marine Integrated Biomedical Technology Center, The National Key Research Institutes in Universities, Pukyong National University, Busan 48513, South Korea., Then AY; Institute of Biological Sciences, Faculty of Science, Universiti Malaya, Kuala Lumpur 50603, Malaysia., Oktavitri NI; Environmental Engineering Study Program, Department of Biology, Faculty of Sciences and Technology, Universitas Airlangga. Jl. Dr. Ir. H. Soekarno, Mulyorejo, Surabaya 60115, East Java, Indonesia., Kim AR; Marine Integrated Biomedical Technology Center, The National Key Research Institutes in Universities, Pukyong National University, Busan 48513, South Korea., Lee SR; Marine Integrated Biomedical Technology Center, The National Key Research Institutes in Universities, Pukyong National University, Busan 48513, South Korea., Pramudya M; Department of Biology, Faculty of Sciences and Technology, Universitas Airlangga. Jl. Dr. Ir. H. Soekarno, Mulyorejo, Surabaya 60115, East Java, Indonesia., Andriyono S; Department of Marine, Faculty of Fisheries and Marine, Universitas Airlangga, Surabaya 60115, Indonesia., Iswara ASW; Department of Biology, Faculty of Sciences and Technology, Universitas Airlangga. Jl. Dr. Ir. H. Soekarno, Mulyorejo, Surabaya 60115, East Java, Indonesia.

Publikováno v: MethodsX [MethodsX] 2024 Nov 05; Vol. 13, pp. 103020. Date of Electronic Publication: 2024 Nov 05 (Print Publication: 2024).