Large-scale Production of Recombinant RNAs on a Circular Scaffold Using a Viroid-derived System in Escherichia coli

Autor: Cordero-Cucart, María Teresa, Aragonés, V., Daros Arnau, Jose Antonio|||0000-0002-6535-2889

Publikováno v: RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
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[EN] With increasing interest in RNA biology and the use of RNA molecules in sophisticated biotechnological applications, the methods to produce large amounts of recombinant RNAs are limited. Here, we describe a protocol to produce large amounts of r

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::05edc7344e8925e9ca3304a3cade9028
https://pubmed.ncbi.nlm.nih.gov/30582599

Automated Behavioral Analysis of Large C. elegans Populations Using a Wide Field-of-view Tracking Platform

Autor: Perni, Michele, Casford, Sam, Aprile, Francesco A, Nollen, Ellen A, Knowles, Tuomas PJ, Vendruscolo, Michele, Dobson, Christopher M

Publikováno v: Journal of visualized experiments : JoVE. JOURNAL OF VISUALIZED EXPERIMENTS
ISSUE=141;ISSN=1940-087X;TITLE=Journal of visualized experiments : JoVE

Caenorhabditis elegans is a well-established animal model in biomedical research, widely employed in functional genomics and ageing studies. To assess the health and fitness of the animals under study, one typically relies on motility readouts, such

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::055383c2bdbcfed1bd3364f8fa2e5eb8

Effect of Fluorescent Proteins on Fusion Partners Using Polyglutamine Toxicity Assays in Yeast

Autor: Martin L. Duennwald, Yuwei Jiang, Sonja E. Di Gregorio, Maram B Albakri, Patrick Lajoie

Publikováno v: Anatomy and Cell Biology Publications

For the investigation of protein localization and trafficking using live cell imaging, researchers often rely on fusing their protein of interest to a fluorescent reporter. The constantly evolving list of genetically encoded fluorescent proteins (FPs

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::01f296a80c7011fbcddd31acf98401e2
https://doi.org/10.3791/58748-v

Gaze in Action: Head-mounted Eye Tracking of Children's Dynamic Visual Attention During Naturalistic Behavior

Autor: John M. Franchak, Tian Linger Xu, Catalina Suarez-Rivera, Chen Yu, Chi-hsin Chen, Lauren K. Slone, Yayun Zhang, Daniel Pearcy, Linda B. Smith, Jeremy I. Borjon, Drew H. Abney

Publikováno v: Journal of visualized experiments : JoVE, vol 2018, iss 141
Slone, LK; Abney, DH; Borjon, JI; Chen, CH; Franchak, JM; Pearcy, D; et al.(2018). Gaze in action: Head-mounted eye tracking of children's dynamic visual attention during naturalistic behavior. Journal of Visualized Experiments, 2018(141). doi: 10.3791/58496. UC Riverside: Retrieved from: http://www.escholarship.org/uc/item/2553z86n
J Vis Exp

© 2018 Journal of Visualized Experiments. Young children's visual environments are dynamic, changing moment-by-moment as children physically and visually explore spaces and objects and interact with people around them. Head-mounted eye tracking offe

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3aab5df2341ea7becddbb333fefe0b7a
https://escholarship.org/uc/item/2553z86n

Use of Pre-Assembled Plastic Microfluidic Chips for Compartmentalizing Primary Murine Neurons

Autor: Valerie Poole, Joseph Harris, Tharkika Nagendran, Anne Marion Taylor

Publikováno v: Journal of visualized experiments : JoVE

Microfabricated methods to compartmentalize neurons have become essential tools for many neuroscientists. This protocol describes the use of a commercially available pre-assembled plastic chip for compartmentalizing cultured primary rat hippocampal n

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::960cecf38c31d554e08b4680a0ffb078
https://doi.org/10.3791/58421-v