Zobrazeno 1 - 10 of 330 pro vyhledávání: '"Issue 136"'
1
A Rapid Image-based Bacterial Virulence Assay Using Amoeba
Autor: Kumar Perinbam, Albert Siryaporn
Publikováno v: Journal of visualized experiments : JoVE. (136)
Traditional bacterial virulence assays involve prolonged exposure of bacteria over the course of several hours to host cells. During this time, bacteria can undergo changes in the physiology due to the exposure to host growth environment and the pres
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::739d7b93a1e43f73614e4f96fe4a7896
https://pubmed.ncbi.nlm.nih.gov/30010653
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2
In Situ Measurement and Correlation of Cell Density and Light Emission of Bioluminescent Bacteria
Autor: Eveline, Brodl, Johannes, Niederhauser, Peter, Macheroux
Publikováno v: Journal of Visualized Experiments : JoVE
There is a considerable number of bacterial species capable of emitting light. All of them share the same gene cluster, namely the lux operon. Despite this similarity, these bacteria show extreme variations in characteristics like growth behavior, in
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::fadc185a1d19ce0ee2b39ddaa09a72f2
https://pubmed.ncbi.nlm.nih.gov/30010658
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3
Adherence of Bacteria to Plant Surfaces Measured in the Laboratory
Autor: Ann G. Matthysse
Publikováno v: Journal of Visualized Experiments : JoVE
This manuscript describes a method to measure bacterial binding to axenic plant surfaces in the light microscope and through the use of viable cell counts. Plant materials used include roots, sprouts, leaves, and cut fruits. The methods described are
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::190155592770653755df3ad3d5bbbae4
https://pubmed.ncbi.nlm.nih.gov/29985316
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4
Diagonal Method to Measure Synergy Among Any Number of Drugs
Autor: Murat Cokol, Melike Cokol-Cakmak, Selim Çetiner, Feray Bakan
Publikováno v: Journal of Visualized Experiments : JoVE
A synergistic drug combination has a higher efficacy compared to the effects of individual drugs. Checkerboard assays, where drugs are combined in many doses, allow sensitive measurement of drug interactions. However, these assays are costly and do n
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::61f5878562ce8870af029cf9e31d8d9f
https://pubmed.ncbi.nlm.nih.gov/29985330
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5
Methods for the Study of Regeneration in Stentor
Autor: Athena, Lin, Tatyana, Makushok, Ulises, Diaz, Wallace F, Marshall
Publikováno v: Journal of Visualized Experiments : JoVE
Cells need to be able to regenerate their parts to recover from external perturbations. The unicellular ciliate Stentor coeruleus is an excellent model organism to study wound healing and subsequent cell regeneration. The Stentor genome became availa
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::e72b34a07903653e33a9670efcb87302
https://pubmed.ncbi.nlm.nih.gov/29985325
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6
Long-term In Vivo Tracking of Inflammatory Cell Dynamics Within Drosophila Pupae
Autor: Helen, Weavers, Anna, Franz, Will, Wood, Paul, Martin
Publikováno v: Journal of Visualized Experiments : JoVE
During the rapid inflammatory response to tissue damage, cells of the innate immune system are quickly recruited to the injury site. Once at the wound, innate immune cells perform a number of essential functions, such as fighting infection, clearing
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::6968f61db7c6dd29306766aabe047f42
https://pubmed.ncbi.nlm.nih.gov/29985351
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7
Evaluating Vascular Hyperpermeability-inducing Agents in the Skin with the Miles Assay
Autor: Alessandro Fantin, Christiana Ruhrberg, James T. Brash
Publikováno v: Journal of Visualized Experiments : JoVE
The primary function of the vascular endothelium in vertebrate organisms is to serve as a barrier between the blood and each tissue of the body, whereby the permeability of the endothelium to blood cells, plasma macromolecules, and water can be adapt
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9d5ea280a92f1ecb3142e673920fbcd1
https://pubmed.ncbi.nlm.nih.gov/29985309
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8
Large-area Scanning Probe Nanolithography Facilitated by Automated Alignment and Its Application to Substrate Fabrication for Cell Culture Studies
Autor: I-Ning, Lee, Joseph, Hosford, Shuai, Wang, John A, Hunt, Judith M, Curran, William P, Heath, Lu Shin, Wong
Publikováno v: Journal of Visualized Experiments : JoVE
Scanning probe microscopy has enabled the creation of a variety of methods for the constructive ('additive') top-down fabrication of nanometer-scale features. Historically, a major drawback of scanning probe lithography has been the intrinsically low
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::acdc9e2c964b66237913883dd83ba14f
https://pubmed.ncbi.nlm.nih.gov/29985313
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9
In Vivo Electrophysiological Measurement of Compound Muscle Action Potential from the Forelimbs in Mouse Models of Motor Neuron Degeneration
Autor: Eveliina, Pollari, Robert, Prior, Wim, Robberecht, Philip, Van Damme, Ludo, Van Den Bosch
Publikováno v: Journal of Visualized Experiments : JoVE
Assessing the functionality of the nerve axon provides detailed information on the progression of neuromuscular disorders. Electrophysiological recordings provide a sensitive approach to measure nerve conduction in humans and rodent models. To broade
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::6325fbaed643b49215959a7ee47b7aab
https://pubmed.ncbi.nlm.nih.gov/29985328
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10
Development and Validation of an Ultrasensitive Single Molecule Array Digital Enzyme-linked Immunosorbent Assay for Human Interferon-α
Autor: Llibre, Alba, Bondet, Vincent, Rodéro, Mathieu, Hunt, David, Crow, Yanick, Duffy, Darragh
Publikováno v: Journal of Visualized Experiments : JoVE
Llibre, A, Bondet, V, Rodero, M P, Hunt, D, Crow, Y J & Duffy, D 2018, ' Development and Validation of an Ultrasensitive Single Molecule Array Digital Enzyme-linked Immunosorbent Assay for Human Interferon-α ', Journal of Visualized Experiments (JoVE), no. 136 . https://doi.org/10.3791/57421
Journal of visualized experiments : JoVE
Journal of visualized experiments : JoVE, 2018, 136, pp.e57421. ⟨10.3791/57421⟩
Journal of visualized experiments : JoVE, JoVE, 2018, pp.e57421. ⟨10.3791/57421⟩
International audience; The main aim of this protocol is to describe the development and validation of an interferon (IFN)-α single molecule array digital Enzyme-Linked ImmunoSorbent Assay (ELISA) assay. This system enables the quantification of hum
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::baf0361d59b18e81ef8a250bc8750c71
https://pubmed.ncbi.nlm.nih.gov/29985347
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