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Combinational Treatment of Trichostatin A and Vitamin C Improves the Efficiency of Cloning Mice by Somatic Cell Nuclear Transfer

Autor: Kei Miyamoto, Masayuki Anzai, Rika Azuma, Mami Oikawa, Masayasu Yamada

Publikováno v: Journal of Visualized Experiments : JoVE

Somatic cell nuclear transfer (SCNT) provides a unique opportunity to directly produce a cloned animal from a donor cell, and it requires the use of skillful techniques. Additionally, the efficiencies of cloning have remained low since the successful

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2639257bce53b105296b225f98101f6a
https://pubmed.ncbi.nlm.nih.gov/29757287

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Assessment of the Effects of Endocrine Disrupting Compounds on the Development of Vertebrate Neural Network Function Using Multi-electrode Arrays

Autor: Karla R Sanchez, Harbinder S Dhillon, Mahlet D Mersha, Murali K. Temburni

Publikováno v: Journal of Visualized Experiments : JoVE

Bis-phenols, such as bis-phenol A (BPA) and bis-phenol-S (BPS), are polymerizing agents widely used in the production of plastics and numerous everyday products. They are classified as endocrine disrupting compounds (EDC) with estradiol-like properti

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1f69c79545a29fdc16c11b3c866467cf
https://pubmed.ncbi.nlm.nih.gov/29757267

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Fabricating Degradable Thermoresponsive Hydrogels on Multiple Length Scales via Reactive Extrusion, Microfluidics, Self-assembly, and Electrospinning

Autor: Fei Xu, Scott B. Campbell, Emilia Bakaic, Daryl Sivakumaran, Eva Mueller, Todd Hoare

Publikováno v: Journal of Visualized Experiments : JoVE

While various smart materials have been explored for a variety of biomedical applications (e.g., drug delivery, tissue engineering, bioimaging, etc.), their ultimate clinical use has been hampered by the lack of biologically-relevant degradation obse

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a8ecfcb58e66374bb1d909cbf4d3ea67
https://pubmed.ncbi.nlm.nih.gov/29708523

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A Protocol for Laboratory Housing of Turquoise Killifish (Nothobranchius furzeri)

Autor: Dodzian, Joanna, Kean, Sam, Seidel, Jens, Valenzano, Dario Riccardo

Publikováno v: J Vis Exp
Journal of Visualized Experiments : JoVE

The development of husbandry practices in non-model laboratory fish used for experimental purposes has greatly benefited from the establishment of reference fish model systems, such as zebrafish and medaka. In recent years, an emerging fish - the tur

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::30da4467c3d3aed2b780e91a66426e7a
https://hdl.handle.net/21.11116/0000-0004-7205-6

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Culturing of Retinal Pigment Epithelial Cells on an Ex Vivo Model of Aged Human Bruch's Membrane

Autor: Hui, Cai, Jie, Gong, Lucian V, Del Priore, Tongalp H, Tezel, Mark A, Fields

Publikováno v: Journal of Visualized Experiments : JoVE

Aside from vitamins and antioxidants recommended by the Age-Related Eye Disease Study, there is no effective therapy for "dry," or atrophic age-related macular degeneration (AMD) which represents 90% of the cases. Therapies are needed to slow or reta

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::2621af6c88d936f4675d8a88cafa352e
https://pubmed.ncbi.nlm.nih.gov/29708536

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Virus Delivery of CRISPR Guides to the Murine Prostate for Gene Alteration

Autor: Maria Riedel, Martin K. Thomsen, Martin F. Berthelsen, Latifa Bakiri, Erwin F. Wagner

Publikováno v: Riedel, M, Berthelsen, M F, Bakiri, L, Wagner, E F & Thomsen, M K 2018, ' Virus delivery of CRISPR guides to the murine prostate for gene alteration ', Journal of Visualized Experiments, vol. 134, e57525 . https://doi.org/10.3791/57525

With an increasing incidence of prostate cancer, identification of new tumor drivers or modulators is crucial. Genetically engineered mouse models (GEMM) for prostate cancer are hampered by tumor heterogeneity and its complex microevolution dynamics.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4415e28bf6c4de6b08b67761abc6aa21
https://doi.org/10.3791/57525-v

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Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence

Autor: Thomas Friedrich, Vivian J. Spiering, Georg Johannes Freiherr von Sass, Nediljko Budisa, Almut Pelzer, Tobias Baumann, Franz-Josef Schmitt

Publikováno v: Journal of Visualized Experiments : JoVE

Fluorescent proteins are fundamental tools for the life sciences, in particular for fluorescence microscopy of living cells. While wild-type and engineered variants of the green fluorescent protein from Aequorea victoria (avGFP) as well as homologs f

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::28eb017c078653ccd59ddf4e41fd665d
https://doi.org/10.3791/57017-v

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Isolation and RNA Extraction of Neurons, Macrophages and Microglia from Larval Zebrafish Brains

Autor: Mazzolini, Julie, Chia, Wen, Sieger, Dirk

Publikováno v: Journal of Visualized Experiments : JoVE
Mazzolini, J, Chia, W & Sieger, D 2018, ' Isolation and RNA Extraction of Neurons, Macrophages and Microglia from Larval Zebrafish Brains ', Journal of Visualized Experiments (JoVE) . https://doi.org/10.3791/57431

To gain a detailed understanding of the role of different CNS cells during development or the establishment and progression of brain pathologies, it is important to isolate these cells without changing their gene expression profile. The zebrafish mod

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ad12602fae9eb6740944d56e3a1ae223
https://doi.org/10.3791/57431-v

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Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease

Publikováno v: Journal of Visualized Experiments : JoVE

Next-generation sequencing (NGS) is quickly revolutionizing how research into the genetic determinants of constitutional disease is performed. The technique is highly efficient with millions of sequencing reads being produced in a short time span and

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ccb2561b93456bf1c3c32fbd78768e8e
https://pubmed.ncbi.nlm.nih.gov/29683450

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Cavernous nerve stimulation and recording of intracavernous pressure in a rat

Autor: Peter Zvara, Lars Lund, Travis Mann-Gow, Morten Hox

Publikováno v: Hox, M, Mann-Gow, T, Lund, L & Zvara, P 2018, ' Cavernous nerve stimulation and recording of intracavernous pressure in a rat ', Journal of Visualized Experiments, vol. 2018, no. 134, e56807 . https://doi.org/10.3791/56807

The stimulation of the cavernous nerve (CN) and measurement of intracavernous pressure (ICP) have been used extensively to test and evaluate therapies for erectile dysfunction. However, the methods used vary between laboratories, and pitfalls still e

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4568eb0aa8f977590aee59c908e2e9bb
https://portal.findresearcher.sdu.dk/da/publications/9d45aaa8-8379-4a88-b05b-f88bb611e888

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