Zobrazeno 1 - 10 of 225 pro vyhledávání: '"Issue 127"'
1
Creation of a Dense Transposon Insertion Library Using Bacterial Conjugation in Enterobacterial Strains Such As Escherichia Coli or Shigella flexneri
Autor: Nikki E, Freed
Publikováno v: Journal of Visualized Experiments : JoVE
Transposon mutagenesis is a method that allows gene disruption via the random genomic insertion of a piece of DNA called a transposon. The protocol below outlines a method for high efficiency transfer between bacterial strains of a plasmid harboring
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::a006cfffd7c1c382d358289c923f27c0
https://pubmed.ncbi.nlm.nih.gov/28994778
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2
A Magnetic Resonance Imaging Protocol for Stroke Onset Time Estimation in Permanent Cerebral Ischemia
Autor: Michael J. Knight, Bryony L. McGarry, Risto A. Kauppinen, Kimmo Jokivarsi, Olli Gröhn
Publikováno v: Journal of Visualized Experiments : JoVE
McGarry, B, Jokivarsi, K T, Knight, M, Grohn, O H J & Kauppinen, R 2017, ' A magnetic resonance imaging protocol for stroke onset time estimation in permanent cerebral ischemia ', Journal of Visualized Experiments, vol. 127, e55277 . https://doi.org/10.3791/55277
MRI provides a sensitive and specific imaging tool to detect acute ischemic stroke by means of a reduced diffusion coefficient of brain water. In a rat model of ischemic stroke, differences in quantitative T1 and T2 MRI relaxation times (qT1 and qT2)
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::43a9f34b239663e195e70e1e421f1701
https://pubmed.ncbi.nlm.nih.gov/28994754
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3
Evaluating Primary Blast Effects In Vitro
Autor: Niall J, Logan, Hari, Arora, Claire A, Higgins
Publikováno v: Journal of Visualized Experiments : JoVE
Exposure to blast events can cause severe trauma to vital organs such as the lungs, ears, and brain. Understanding the mechanisms behind such blast-induced injuries is of great importance considering the recent trend towards the use of explosives in
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::5606129234482ae0e1fcf59846c79517
https://pubmed.ncbi.nlm.nih.gov/28994788
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4
A Novel Method: Super-selective Adrenal Venous Sampling
Autor: Tsugio Seki, Kohzoh Makita, Tetsuo Nishikawa, Shigehiro Karashima, Kanako Kiriyama-Kitamoto, Masanori Yasuda, Seishi Matsui, Koshiro Nishimoto, Masao Omura
Publikováno v: Journal of Visualized Experiments : JoVE
Primary aldosteronism (PA) and subclinical Cushing's syndrome (SCS) are conditions in which the adrenal glands autonomously produce excessive amounts of aldosterone and cortisol, respectively. The conventional adrenal venous sampling (cAVS) method co
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::93c3153cf5e350e29333c3f09c98c7a4
https://pubmed.ncbi.nlm.nih.gov/28994759
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5
The Knob Supination Task: A Semi-automated Method for Assessing Forelimb Function in Rats
Autor: Samuel D. Butensky, Robert L. Rennaker, Andrew M. Sloan, Eric C. Meyers, Joshua Santos, Thelma Bethea, Anil Sindhurakar, Jason B. Carmel
Publikováno v: Journal of Visualized Experiments : JoVE
Tasks that accurately measure dexterity in animal models are critical to understand hand function. Current rat behavioral tasks that measure dexterity largely use video analysis of reaching or food manipulation. While these tasks are easy to implemen
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6b25b87d9fc9d9db183c36e4fcf62595
https://doi.org/10.3791/56341
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6
An Experimental Protocol for Assessing the Performance of New Ultrasound Probes Based on CMUT Technology in Application to Brain Imaging
Autor: Fabio Quaglia, Alessandro Stuart Savoia, Giulia Matrone, Gloria Castellazzi, Marco Piastra, Alessandro Ramalli, Patrizia Morbini
Publikováno v: Journal of Visualized Experiments.
The possibility to perform an early and repeatable assessment of imaging performance is fundamental in the design and development process of new ultrasound (US) probes. Particularly, a more realistic analysis with application-specific imaging targets
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2fc27107e58a4e6eedf7c8dfc8a60a23
https://doi.org/10.3791/55798-v
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7
Fourier-Based Diffraction Analysis of Live Caenorhabditis elegans
Autor: Jenny, Magnes, Harold M, Hastings, Kathleen M, Raley-Susman, Clara, Alivisatos, Adam, Warner, Miranda, Hulsey-Vincent
Publikováno v: Journal of Visualized Experiments : JoVE
This manuscript describes how to classify nematodes using temporal far-field diffraction signatures. A single C. elegans is suspended in a water column inside an optical cuvette. A 632 nm continuous wave HeNe laser is directed through the cuvette usi
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::5034f533169bae5e78d5402d0e1a5ad0
https://pubmed.ncbi.nlm.nih.gov/28930977
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8
Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method
Autor: Gail M. Nelson, Brian N. Chorley, Jenna M Guynn
Publikováno v: Journal of Visualized Experiments : JoVE
New technologies that utilize capillary-based immunoassays promise faster and more quantitative protein assessment compared to traditional immunoassays. However, similar to other antibody-based protein assays, optimization of capillary-based immunoas
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7cceb5bba8da8042c1e5e069a0aff64c
https://pubmed.ncbi.nlm.nih.gov/28930974
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9
Preparation of Liquid Crystal Networks for Macroscopic Oscillatory Motion Induced by Light
Autor: Dirk J. Broer, Ghislaine Vantomme, Anne Helene Gelebart, E. W. Meijer
Publikováno v: Journal of Visualized Experiments (JoVE), 2017(127):e56266, 1-9. MYJoVE Corporation
Journal of Visualized Experiments
Journal of Visualized Experiments : JoVE
A strategy based on doped liquid crystalline networks is described to create mechanical self-sustained oscillations of plastic films under continuous light irradiation. The photo-excitation of dopants that can quickly dissipate light into heat, coupl
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::269d7d1bb7f041adc6cceb41f671717d
https://doi.org/10.3791/56266
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10
Generation of Prostate Cancer Cell Models of Resistance to the Anti-mitotic Agent Docetaxel
Autor: Jungreem Woo, Carlos Cordon-Cardo, Lisa Mohr, Marc Carceles-Cordon, Veronica Rodriguez-Bravo, Josep Domingo-Domenech
Publikováno v: Journal of Visualized Experiments : JoVE
Microtubule targeting agents (MTAs) are a mainstay in the treatment of a wide range of tumors. However, acquired resistance to chemotherapeutic drugs is a common mechanism of disease progression and a prognostic-determinant feature of malignant tumor
Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2b69083f52ca5e3bf8ea2fd0080df9ce
https://doi.org/10.3791/56327
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