Výsledky vyhledávání - "Isslam Bouazzaoui"

Supplementary Figure 4. (A-D) from Combining Homologous Recombination and Phosphopeptide-binding Data to Predict the Impact of BRCA1 BRCT Variants on Cancer Risk

Autor: Sandrine M. Caputo, Sophie Zinn-Justin, Bernard S. Lopez, Rosette Lidereau, Etienne Rouleau, Catherine Noguès, Jean-Christophe Aude, Philippe Lafitte, Jeff A. Schnell, Jean Feunteun, Dorine Bonte, Isslam Bouazzaoui, Patrick Julien, Josée Guirouilh-Barbat, Naima Nhiri, Eric Jacquet, Elodie Dardillac, Ambre Petitalot

Isothermal Titration Calorimetry curves obtained by adding the different phosphopeptides at (A-B) 200 mM onto the WT BRCT domains at 20 mM and (C-D) 100 mM onto the WT BRCT domains at 10 mM .

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::22dec69eab22ab3b11280be35673d603
https://doi.org/10.1158/1541-7786.22516320.v1

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Data from Combining Homologous Recombination and Phosphopeptide-binding Data to Predict the Impact of BRCA1 BRCT Variants on Cancer Risk

BRCA1 mutations have been identified that increase the risk of developing hereditary breast and ovarian cancers. Genetic screening is now offered to patients with a family history of cancer, to adapt their treatment and the management of their relati

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::b45b9eb3f37bba3880f02c4ad9363ac2
https://doi.org/10.1158/1541-7786.c.6541344

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Supplementary Figure 3. Classification of the 42 purified mutated BRCT domains as a function of their thermostability as measured using a high throughput fluorescence assay. from Combining Homologous Recombination and Phosphopeptide-binding Data to Predict the Impact of BRCA1 BRCT Variants on Cancer Risk

The black bar corresponds to WT BRCA1, the blue bars to VUS of classes 1 and 2, the grey bars to VUS of class 3 and the red bars to VUS of classes 4 and 5. Bars boxed in green correspond to mutants that are defective in phosphopeptide-binding, as obs

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::09cebf5b95634847f2937299504bfb5b
https://doi.org/10.1158/1541-7786.22516326.v1

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Supplementary Figure 6. (A) from Combining Homologous Recombination and Phosphopeptide-binding Data to Predict the Impact of BRCA1 BRCT Variants on Cancer Risk

FBTSA assay revealing that binding of the WT BRCA1 BRCT domains to ACC1-P, BACH1-P, CtiP-P, AB-1P and AB-2P induce a measurable increase in the BRCT thermostability that raises with peptide concentration.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::02a03158dbebb5c31bc66fd342597e32
https://doi.org/10.1158/1541-7786.22516314

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Supplementary Figure 2. Classification of the VUS as a function of the impact of the corresponding missense variations on BRCT domain expression in E. coli. from Combining Homologous Recombination and Phosphopeptide-binding Data to Predict the Impact of BRCA1 BRCT Variants on Cancer Risk

Mutated BRCT domains fused to GST were expressed in E. coli and purified by affinity chromatography using glutathione beads. This figure shows a SDS-PAGE gel with samples from the bacterial pellet (P), supernatant (S) and the glutathione beads (G) af

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::927bafcd0af3c9d874bd9e4737f7cad7
https://doi.org/10.1158/1541-7786.22516332

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Supplementary Figure 5. (A-B) from Combining Homologous Recombination and Phosphopeptide-binding Data to Predict the Impact of BRCA1 BRCT Variants on Cancer Risk

Size Exclusion Chromatography profiles obtained on BRCA1 BRCT domains free (red curve) or in complex with BACH1-P (purple), AB-1P (green), AB-2P (blue). The experiment was performed using a Superdex-75 10/300 GL column (GE Healthcare) pre-equilibrate

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::942b70e5108f3e81c42b65b49d78883d
https://doi.org/10.1158/1541-7786.22516317.v1

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Supplementary Figure 6. (B-C) from Combining Homologous Recombination and Phosphopeptide-binding Data to Predict the Impact of BRCA1 BRCT Variants on Cancer Risk

FBTSA assays performed to measure binding of the mutated BRCA1 BRCT domains to ACC1-P, BACH1-P, CtiP-P, AB-1P and AB-2P.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9bcb0ea74fec9143ceb078c686be4488
https://doi.org/10.1158/1541-7786.22516311.v1

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Combining Homologous Recombination and Phosphopeptide-binding Data to Predict the Impact of

Autor: Ambre, Petitalot, Elodie, Dardillac, Eric, Jacquet, Naima, Nhiri, Josée, Guirouilh-Barbat, Patrick, Julien, Isslam, Bouazzaoui, Dorine, Bonte, Jean, Feunteun, Jeff A, Schnell, Philippe, Lafitte, Jean-Christophe, Aude, Catherine, Noguès, Etienne, Rouleau, Rosette, Lidereau, Bernard S, Lopez, Sophie, Zinn-Justin, Sandrine M, Caputo

Publikováno v: Molecular cancer research : MCR. 17(1)

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=pmid________::dbc7a7bf9ba6794c009e850ec9b64728
https://pubmed.ncbi.nlm.nih.gov/30257991

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