Posttranslational, site-directed photochemical fluorine editing of protein sidechains to probe residue oxidation state via 19F-nuclear magnetic resonance

Autor: Isenegger, PG, Josephson, B, Gaunt, B, Davy, MJ, Gouverneur, V, Baldwin, AJ, Davis, BG

Publikováno v: Nature Protocols. 18:1543-1562

The fluorination of amino acid residues represents a near-isosteric alteration with the potential to report on biological pathways, yet the site-directed editing of carbon–hydrogen (C–H) bonds in complex biomolecules to carbon–fluorine (C–F)

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::da881b55638824ff5e20852b6acaef1c
https://doi.org/10.1038/s41596-022-00800-9

Publisher Correction: Post-translational insertion of boron in proteins to probe and modulate function.

Autor: Mollner TA; Department of Chemistry, Chemistry Research Laboratory, University of Oxford, Oxford, UK., Isenegger PG; Department of Chemistry, Chemistry Research Laboratory, University of Oxford, Oxford, UK., Josephson B; Department of Chemistry, Chemistry Research Laboratory, University of Oxford, Oxford, UK., Buchanan C; Department of Chemistry, Physical and Theoretical Chemistry Laboratory, University of Oxford, Oxford, UK., Lercher L; Department of Chemistry, Chemistry Research Laboratory, University of Oxford, Oxford, UK., Oehlrich D; Neuroscience Medicinal Chemistry, Janssen Research and Development, Beerse, Belgium., Hansen DF; Division of Biosciences, University College London, London, UK., Mohammed S; Department of Chemistry, Chemistry Research Laboratory, University of Oxford, Oxford, UK.; Department of Biochemistry, University of Oxford, Oxford, UK.; The Rosalind Franklin Institute, Oxfordshire, Oxford, UK., Baldwin AJ; Department of Chemistry, Physical and Theoretical Chemistry Laboratory, University of Oxford, Oxford, UK.; The Rosalind Franklin Institute, Oxfordshire, Oxford, UK., Gouverneur V; Department of Chemistry, Chemistry Research Laboratory, University of Oxford, Oxford, UK., Davis BG; Department of Chemistry, Chemistry Research Laboratory, University of Oxford, Oxford, UK. Ben.Davis@rfi.ac.uk.; The Rosalind Franklin Institute, Oxfordshire, Oxford, UK. Ben.Davis@rfi.ac.uk.

Publikováno v: Nature chemical biology [Nat Chem Biol] 2022 Jan; Vol. 18 (1), pp. 116.

Residue-Selective Protein C-Formylation via Sequential Difluoroalkylation-Hydrolysis.

Autor: Imiołek M; Department of Chemistry, University of Oxford, Oxford OX1 3TA, United Kingdom of Great Britain and Northern Ireland.; The Rosalind Franklin Institute, Oxfordshire OX11 0FA, United Kingdom of Great Britain and Northern Ireland., Isenegger PG; Department of Chemistry, University of Oxford, Oxford OX1 3TA, United Kingdom of Great Britain and Northern Ireland., Ng WL; Department of Chemistry, University of Oxford, Oxford OX1 3TA, United Kingdom of Great Britain and Northern Ireland., Khan A; Department of Chemistry, University of Oxford, Oxford OX1 3TA, United Kingdom of Great Britain and Northern Ireland., Gouverneur V; Department of Chemistry, University of Oxford, Oxford OX1 3TA, United Kingdom of Great Britain and Northern Ireland., Davis BG; Department of Chemistry, University of Oxford, Oxford OX1 3TA, United Kingdom of Great Britain and Northern Ireland.; The Rosalind Franklin Institute, Oxfordshire OX11 0FA, United Kingdom of Great Britain and Northern Ireland.

Publikováno v: ACS central science [ACS Cent Sci] 2021 Jan 27; Vol. 7 (1), pp. 145-155. Date of Electronic Publication: 2021 Jan 13.

Light-driven post-translational installation of reactive protein side chains.

Autor: Josephson B; Chemistry Research Laboratory, Department of Chemistry, University of Oxford, Oxford, UK., Fehl C; Chemistry Research Laboratory, Department of Chemistry, University of Oxford, Oxford, UK.; Department of Chemistry, Wayne State University, Detroit, MI, USA., Isenegger PG; Chemistry Research Laboratory, Department of Chemistry, University of Oxford, Oxford, UK., Nadal S; Chemistry Research Laboratory, Department of Chemistry, University of Oxford, Oxford, UK., Wright TH; Chemistry Research Laboratory, Department of Chemistry, University of Oxford, Oxford, UK.; Department of Molecular Biology, Massachusetts General Hospital, Boston, MA, USA., Poh AWJ; Chemistry Research Laboratory, Department of Chemistry, University of Oxford, Oxford, UK., Bower BJ; Chemistry Research Laboratory, Department of Chemistry, University of Oxford, Oxford, UK., Giltrap AM; Chemistry Research Laboratory, Department of Chemistry, University of Oxford, Oxford, UK.; The Rosalind Franklin Institute, Harwell, UK., Chen L; Physical and Theoretical Chemistry Laboratory, Department of Chemistry, University of Oxford, Oxford, UK., Batchelor-McAuley C; Physical and Theoretical Chemistry Laboratory, Department of Chemistry, University of Oxford, Oxford, UK., Roper G; Chemistry Research Laboratory, Department of Chemistry, University of Oxford, Oxford, UK., Arisa O; Chemistry Research Laboratory, Department of Chemistry, University of Oxford, Oxford, UK., Sap JBI; Chemistry Research Laboratory, Department of Chemistry, University of Oxford, Oxford, UK., Kawamura A; Chemistry Research Laboratory, Department of Chemistry, University of Oxford, Oxford, UK., Baldwin AJ; Chemistry Research Laboratory, Department of Chemistry, University of Oxford, Oxford, UK., Mohammed S; Chemistry Research Laboratory, Department of Chemistry, University of Oxford, Oxford, UK.; The Rosalind Franklin Institute, Harwell, UK.; Department of Biochemistry, University of Oxford, Oxford, UK., Compton RG; Physical and Theoretical Chemistry Laboratory, Department of Chemistry, University of Oxford, Oxford, UK., Gouverneur V; Chemistry Research Laboratory, Department of Chemistry, University of Oxford, Oxford, UK. veronique.gouverneur@chem.ox.ac.uk., Davis BG; Chemistry Research Laboratory, Department of Chemistry, University of Oxford, Oxford, UK. ben.davis@rfi.ac.uk.; The Rosalind Franklin Institute, Harwell, UK. ben.davis@rfi.ac.uk.

Publikováno v: Nature [Nature] 2020 Sep; Vol. 585 (7826), pp. 530-537. Date of Electronic Publication: 2020 Sep 23.

Manual and automated Cu-mediated radiosynthesis of the PARP inhibitor [ ¹⁸ F]olaparib.

Autor: Guibbal F; Chemistry Research Laboratory, University of Oxford, Oxford, UK.; Radiobiology Research Institute, Department of Oncology, Churchill Hospital, University of Oxford, Headington, UK., Isenegger PG; Chemistry Research Laboratory, University of Oxford, Oxford, UK., Wilson TC; Chemistry Research Laboratory, University of Oxford, Oxford, UK., Pacelli A; Radiobiology Research Institute, Department of Oncology, Churchill Hospital, University of Oxford, Headington, UK., Mahaut D; Chemistry Research Laboratory, University of Oxford, Oxford, UK., Sap JBI; Chemistry Research Laboratory, University of Oxford, Oxford, UK., Taylor NJ; Chemistry Research Laboratory, University of Oxford, Oxford, UK., Verhoog S; Chemistry Research Laboratory, University of Oxford, Oxford, UK., Preshlock S; Chemistry Research Laboratory, University of Oxford, Oxford, UK., Hueting R; Radiobiology Research Institute, Department of Oncology, Churchill Hospital, University of Oxford, Headington, UK., Cornelissen B; Radiobiology Research Institute, Department of Oncology, Churchill Hospital, University of Oxford, Headington, UK. bart.cornelissen@oncology.ox.ac.uk., Gouverneur V; Chemistry Research Laboratory, University of Oxford, Oxford, UK. veronique.gouverneur@chem.ox.ac.uk.

Publikováno v: Nature protocols [Nat Protoc] 2020 Apr; Vol. 15 (4), pp. 1525-1541. Date of Electronic Publication: 2020 Feb 28.