Zobrazeno 1 - 10
of 147
pro vyhledávání: '"Isao, Masuda"'
Autor:
Isao Masuda, Ya-Ming Hou
Publikováno v:
Frontiers in Microbiology, Vol 15 (2024)
Interpretation of the genetic code from triplets of nucleotides to amino acids is fundamental to life. This interpretation is achieved by cellular tRNAs, each reading a triplet codon through its complementary anticodon (positions 34–36) while deliv
Externí odkaz:
https://doaj.org/article/e7999c18706a4df0bab901e5090e3fc7
Autor:
Isao Masuda, Ya-Ming Hou
Publikováno v:
STAR Protocols, Vol 4, Iss 2, Pp 102196- (2023)
Summary: We describe here a genome-wide screening approach to identify the most critical core reaction among a network of many that are supported by an essential gene to establish cell viability. We describe steps for maintenance plasmid construction
Externí odkaz:
https://doaj.org/article/d227eb1c038446f6b2165a56186e9a8c
Autor:
Gabriel Demo, Howard B. Gamper, Anna B. Loveland, Isao Masuda, Christine E. Carbone, Egor Svidritskiy, Ya-Ming Hou, Andrei A. Korostelev
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-12 (2021)
Translational frameshifting is a mechanism that expands the coding capabilities of mRNA. Here, structures of 70S ribosome complexes with GTPase elongation factor G (EF-G), a +1-frameshifting-prone mRNA and tRNAs reveal the cooperation between the rib
Externí odkaz:
https://doaj.org/article/9e96fbbefbf946cca2e64e5aa10017b3
Autor:
Howard Gamper, Haixing Li, Isao Masuda, D. Miklos Robkis, Thomas Christian, Adam B. Conn, Gregor Blaha, E. James Petersson, Ruben L. Gonzalez, Ya-Ming Hou
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-18 (2021)
Genome recoding with quadruplet codons requires a +1-frameshift-suppressor tRNA able to insert an amino acid at quadruplet codons of interest. Here the authors identify the mechanisms resulting in +1 frameshifting and the steps of the elongation cycl
Externí odkaz:
https://doaj.org/article/f25e7830b887458f96ecdebbbd086160
Autor:
Isao Masuda, Jae-Yeon Hwang, Thomas Christian, Sunita Maharjan, Fuad Mohammad, Howard Gamper, Allen R Buskirk, Ya-Ming Hou
Publikováno v:
eLife, Vol 10 (2021)
N1-methylation of G37 is required for a subset of tRNAs to maintain the translational reading-frame. While loss of m1G37 increases ribosomal +1 frameshifting, whether it incurs additional translational defects is unknown. Here, we address this questi
Externí odkaz:
https://doaj.org/article/a833a1e94d0c41389028df927ab81e6b
Autor:
Isao Masuda, Masato Koike, Shohei Nakashima, Yu Mizutani, Yusuke Ozawa, Kenji Watanabe, Yoko Sawada, Hiroshi Sugiyama, Atsushi Sugimoto, Hidetoshi Nojiri, Koichi Sashihara, Koutaro Yokote, Takahiko Shimizu
Publikováno v:
Scientific Reports, Vol 8, Iss 1, Pp 1-13 (2018)
Abstract Apples are well known to have various benefits for the human body. Procyanidins are a class of polyphenols found in apples that have demonstrated effects on the circulatory system and skeletal organs. Osteoarthritis (OA) is a locomotive synd
Externí odkaz:
https://doaj.org/article/807631a87310403fad916501ca20c8ed
Publikováno v:
Frontiers in Genetics, Vol 9 (2019)
Although the genetic code is degenerate, synonymous codons for the same amino acid are not translated equally. Codon-specific translation is important for controlling gene expression and determining the proteome of a cell. At the molecular level, cod
Externí odkaz:
https://doaj.org/article/c3dbdbba3d2e40b1a873f9168893d86c
Autor:
Howard Gamper, Yuhong Wang, Henri McGuigan, Gregor Blaha, Yujia Mao, Shoujun Xu, Ya-Ming Hou, Isao Masuda
Publikováno v:
Nucleic Acids Research
Inducing tRNA +1 frameshifting to read a quadruplet codon has the potential to incorporate a non-natural amino acid into the polypeptide chain. While this strategy is being considered for genome expansion in biotechnology and bioengineering endeavors
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::db9056d163fcc1a8262ee93cdb224748
https://doi.org/10.1093/nar/gkab734
https://doi.org/10.1093/nar/gkab734
Autor:
Gregor Blaha, Ya-Ming Hou, Thomas Christian, D. Miklos Robkis, Haixing Li, Howard Gamper, Isao Masuda, Adam B. Conn, Ruben L. Gonzalez, E. James Petersson
Publikováno v:
Nature Communications, Vol 12, Iss 1, Pp 1-18 (2021)
Nature Communications
Nature Communications
While genome recoding using quadruplet codons to incorporate non-proteinogenic amino acids is attractive for biotechnology and bioengineering purposes, the mechanism through which such codons are translated is poorly understood. Here we investigate t
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c516a2acacf45128e54e4d770c83a143
https://doaj.org/article/f25e7830b887458f96ecdebbbd086160
https://doaj.org/article/f25e7830b887458f96ecdebbbd086160
Autor:
Isao Masuda, Yuka Yamaki, Rajesh Detroja, Somnath Tagore, Henry Moore, Sunita Maharjan, Yuko Nakano, Thomas Christian, Ryuma Matsubara, Todd Lowe, Milana Frenkel-Morgenstern, Ya-Ming Hou
Publikováno v:
Masuda, Isao; Yamaki, Yuka; Detroja, Rajesh; Tagore, Somnath; Moore, Henry; Maharjan, Sunita; Nakano, Yuko; Christian, Thomas; Matsubara, Ryuma; Lowe, Todd M; Frenkel-Morgenstern, Milana; Hou, Ya-Ming (2022). tRNA methylation resolves codon usage bias at the limit of cell viability. Cell reports, 41(4), p. 111539. Cell Press 10.1016/j.celrep.2022.111539
SummaryThe codon usage of each genome is closely correlated with the abundance of tRNA isoacceptors. How codon-usage bias is resolved by tRNA post-transcriptional modifications is largely unknown. Here we demonstrate that the N1-methylation of guanos
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::31a5bd8a6048f867f2cad7c6cc6889e9
https://doi.org/10.1101/2022.05.14.491930
https://doi.org/10.1101/2022.05.14.491930
