Výsledky vyhledávání - "Isabelle O'Callaghan"

Validation of a Lysis Buffer Containing 4 M Guanidinium Thiocyanate (GITC)/ Triton X-100 for Extraction of SARS-CoV-2 RNA for COVID-19 Testing: Comparison of Formulated Lysis Buffers Containing 4 to 6 M GITC, Roche External Lysis Buffer and Qiagen RTL Lysis Buffer

Autor: Paula M. O'Connor, John McSharry, Brigid Lucey, Sarah P. Hudson, Martina F. Scallan, Isabelle O'Callaghan, Conor P. Horgan, Paul D. Cotter, Humphrey A. Moynihan, Edel Durack, Catherine Dempsey

The COVID-19 pandemic has resulted in increased need for diagnostic testing using reverse transcriptase real-time PCR (RT-PCR). An exponential increase in demand has resulted in a shortage of numerous reagents in particular those associated with the

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2af2a9c5caf62dc80f570829d7325bb3

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Improved Detection of Bacterial Pathogens in Patients Presenting with Gastroenteritis by Use of the EntericBio Real-Time Gastro Panel I Assay

Autor: Brigid Lucey, Monika Koziel, Gerard D. Corcoran, Roy D. Sleator, Rachel Kiely, Liam Blake, Isabelle O'Callaghan

Publikováno v: Journal of Clinical Microbiology. 51:2679-2685

In this study, we evaluated the use of EntericBio real-time Gastro Panel I (Serosep, Limerick, Ireland) for routine use in a clinical microbiology laboratory for simultaneous detection of Campylobacter jejuni , coli , and lari , Shiga toxin-producing

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b5f9ae8e4534eee2566910e0a521a898
https://doi.org/10.1128/jcm.00809-13

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Validation of the EntericBio Panel II® multiplex polymerase chain reaction system for detection of Campylobacter spp., Salmonella spp., Shigella spp., and verotoxigenic E. coli for use in a clinical diagnostic setting

Autor: Monika Koziel, Roy D. Sleator, Isabelle O'Callaghan, Dan Corcoran, Brigid Lucey

Publikováno v: Diagnostic Microbiology and Infectious Disease. 75:46-49

A total of 717 faeces samples were tested prospectively using the EntericBio Panel II® detection system (Serosep, Limerick, Ireland), in parallel with routine laboratory testing, which combines the EntericBio® system with retrospective culture of e

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::4bd87513785fd993a7cd04ca0e42651a
https://doi.org/10.1016/j.diagmicrobio.2012.09.007

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Stool consistency: no hard and fast rules for the diagnosis of VTEC infection

Autor: Daniel Corcoran, Shirley Ross, Isabelle O'Callaghan, Liam Blake, Brigid Lucey, Roy D. Sleator, Rachel Jermy

Publikováno v: Journal of Clinical Pathology. 69:837-837

Verotoxigenic Escherichia coli (VTEC) is a serious gastrointestinal pathogen associated with significant morbidity and mortality in developed countries1 and the Centers for Disease Control and Prevention (CDC) recommends that the ideal stool sample f

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2fc658d4b8c37da7a7b74edd2cf10b3e
https://doi.org/10.1136/jclinpath-2016-203792

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Design of a multiplex PCR assay for the simultaneous detection and confirmation of Neisseria gonorrhoeae

Autor: Daniel Corcoran, Brigid Lucey, Isabelle O'Callaghan

Publikováno v: Journal of clinical pathology. 63(5)

ObjectivesTo improve the detection of Neisseria gonorrhoeae by designing a multiplex PCR assay using two N gonorrhoeae-specific genes as targets, thereby providing detection and confirmation of a positive result simultaneously.MethodsPCR primers were

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6a6f74b9c66f13c2cf0af1748a51ec68
https://pubmed.ncbi.nlm.nih.gov/20360139

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