Zobrazeno 1 - 10
of 10
pro vyhledávání: '"Isaac Sadnik"'
Publikováno v:
Biochemistry. 16:2221-2230
A population of free, native ribosomal 40S subunits, that do not react with 60S subunits to form 80S ribosomes, has been identified in the postmicrosomal fraction of rat liver homogenates. A protein (IF-3) has been purified from high salt (0.88 M KCI
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::11690bb194f4b39ac9591b253aa3b116
https://doi.org/10.1021/bi00629a028
https://doi.org/10.1021/bi00629a028
Publikováno v:
Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression. 782:220-227
The effects of incubation of yeast spheroplasts at elevated temperature (40 degrees C) on a number of activities involved in protein biosynthesis have been examined in preparations obtained from wild-type cells (wt A364A ) and a temperature-sensitive
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7f07a5766f561836f8c286bb1c29fdec
https://doi.org/10.1016/0167-4781(84)90027-7
https://doi.org/10.1016/0167-4781(84)90027-7
Publikováno v:
Archives of Biochemistry and Biophysics. 199:277-285
Extracts prepared from native ribosomal subunits of rat liver with high salt (0.5 m KCl) solutions contain dissociation factor activity; they dissociate 80 S ribosomes and prevent the reassociation of ribosomal subunits. One of the activities is a hi
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9af6aa4b5198cce4a71c621082a34dfe
https://doi.org/10.1016/0003-9861(80)90282-9
https://doi.org/10.1016/0003-9861(80)90282-9
Publikováno v:
Biochemical and Biophysical Research Communications. 73:532-538
An assay for factor-dependent ribosome dissociation has been developed, by coupling with the peptidyltransferase reaction between acylaminoacyl-tRNAs and puromycin. The peptidyltransferase reaction is specific for 60S subunits; it is inhibited by der
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c7ec0f9b4dcaf5c92a329c497b842194
https://doi.org/10.1016/0006-291x(76)90739-7
https://doi.org/10.1016/0006-291x(76)90739-7
Publikováno v:
Biochemistry. 14:5328-5335
The binding of the initiator tRNA Met-tRNAf, and of acetylphenylalanyl-tRNA, has been examined with rat liver 40S subunits derived from 80S ribosomes by dissociation with native 40S subunits sedimented from the postmicrosomal fraction and with native
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9b37cfa726695aeb2a3eec47e4a83e6d
https://doi.org/10.1021/bi00695a017
https://doi.org/10.1021/bi00695a017
Publikováno v:
Biochemistry. 16:4664-4672
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::8b73ffce2e2b8197e5e627a375b64cad
https://doi.org/10.1021/bi00640a021
https://doi.org/10.1021/bi00640a021
Autor:
Isaac Sadnik, Nancy Moreno, José Martínez, Calvin S. McLaughlin, B Feinberg, Kivie Moldave, Flor Herrera
Publikováno v:
Journal of Biological Chemistry. 259:14347-14349
Postpolysomal extracts from wild-type (wt A364A) and temperature-sensitive (ts 7'-14) yeast cells were preincubated for short periods of time at the nonpermissive temperature (37-41 degrees C) prior to incubations for protein synthesis at 20 degrees
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::4127651c66d20198b08b76e512dc4926
https://doi.org/10.1016/s0021-9258(17)42602-0
https://doi.org/10.1016/s0021-9258(17)42602-0
Publikováno v:
The Journal of biological chemistry. 254(10)
A cell-free protein-synthesizing system has been prepared from Saccharomyces cerevisiae by differential centrifugation of lysed spheroplasts. The preparation, a modified 100,000 x g supernatant fraction, contains ribosomes and monosomes, ribosomal su
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b562ab2f2e01437b5500838778da5f60
https://pubmed.ncbi.nlm.nih.gov/374404
https://pubmed.ncbi.nlm.nih.gov/374404
Autor:
Kivie Moldave, Isaac Sadnik
Publikováno v:
Methods in Enzymology ISBN: 9780121819590
Publisher Summary The chapter discusses the preparation of derived and native ribosomal subunits from rat liver. To prepare ribosomal 40 S and 60 S subunits derived from polysomes (designated d40S and d60S subunits), the microsomes obtained from the
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_________::b31379b1dff929486a6cd3af94af87c3
https://doi.org/10.1016/0076-6879(79)59101-0
https://doi.org/10.1016/0076-6879(79)59101-0
