Zobrazeno 1 - 10
of 11
pro vyhledávání: '"Isa M. Stehle"'
Autor:
Marialuisa Lavitrano, Alessia Vargiolu, Augusta Zannoni, Maria Grazia Cerrito, Hans J. Lipps, Pierluigi Donini, Roberto Giovannoni, Isa M. Stehle, Monica Forni, Stefano Manzini, Michele Papa, Maria Laura Bacci, Maria Rosaria Bianco
Publikováno v:
Proceedings of the National Academy of Sciences. 103:17672-17677
Genetic modification of cells and animals is an invaluable tool for biotechnology and biomedicine. Currently, integrating vectors are used for this purpose. These vectors, however, may lead to insertional mutagenesis and variable transgene expression
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::08f2b9a5919047efe2e8f71f923183f3
https://doi.org/10.1073/pnas.0604938103
https://doi.org/10.1073/pnas.0604938103
Publikováno v:
Molecular Biology Reports. 31:85-90
Currently available vectors for mammalian cells suffer from a number of limitations which make them only partially useful for genetic modification of eukaryotic cells and organisms and for gene therapy. While integration of a vector can lead to unpre
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::2fc31f5d82af1c207262cd6c907be052
https://doi.org/10.1023/b:mole.0000031363.35839.46
https://doi.org/10.1023/b:mole.0000031363.35839.46
Publikováno v:
The EMBO Journal. 23:191-201
An extrachromosomally replicating plasmid was used to investigate the specificity by which the origin recognition complex (ORC) interacts with DNA sequences in mammalian cells in vivo. We first showed that the plasmid pEPI-1 replicates semiconservati
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ed3a6b9da678bc7d8f7c4442e97d8f34
https://doi.org/10.1038/sj.emboj.7600029
https://doi.org/10.1038/sj.emboj.7600029
Publikováno v:
Gene. 304:23-33
Currently used vectors in human gene therapy suffer from a number of limitations with respect to safety and reproducibility. There is increasing agreement that the ideal vector for gene therapy should be completely based on chromosomal elements and b
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f339b30429d084a23d066694850378ae
https://doi.org/10.1016/s0378-1119(02)01215-5
https://doi.org/10.1016/s0378-1119(02)01215-5
Publikováno v:
Human gene therapy. 16(4)
The inhibition of gene expression by RNA interference harbors a high potential for application in the therapy of human diseases. However, while exogenous application of siRNAs efficiently inhibits gene expression, these effects are only transient in
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aa1beb5d4aab0929f366087f35af4115
https://pubmed.ncbi.nlm.nih.gov/15871685
https://pubmed.ncbi.nlm.nih.gov/15871685
Autor:
Frank O. Fackelmayer, Tobias Eisenberger, Isa M. Stehle, Frank Herrmann, Armin Baiker, Andreas Jenke, Jürgen Bode, Hans J. Lipps
The activation of mammalian origins of replication depends so far on ill understood epigenetic events, such as binding of transcription factors, chromatin structure, and nuclear localization. Understanding these mechanisms is not only a scientific ch
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::7c115238a2ff45be9bd96e63f621b60b
https://europepmc.org/articles/PMC509201/
https://europepmc.org/articles/PMC509201/
Publikováno v:
Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology. 11(5)
In order to analyze epigenetic factors involved in the regulation of DNA replication in higher eukaryotic cells, minimal systems have to be established. We have recently constructed a non-viral episomal vector system which replicates episomally in ma
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=pmid________::8d20b458b9ee99324dc92911aa26e4cf
https://pubmed.ncbi.nlm.nih.gov/12971718
https://pubmed.ncbi.nlm.nih.gov/12971718
Autor:
Frank O. Fackelmayer, Franziska Jönsson, Harald Conradt, Jürgen Bode, Bok Hee C Jenke, Hans J. Lipps, Christian Fetzer, Isa M. Stehle
pEPI-1, a vector in which a chromosomal scaffold/matrix-attached region (S/MAR) is linked to the simian virus 40 origin of replication, is propagated episomally in CHO cells in the absence of the virally encoded large T-antigen and is stably maintain
Externí odkaz:
https://explore.openaire.eu/search/publication?articleId=doi_dedup___::282c8ff8dd8e45492f6340ea4e7481e1
https://hdl.handle.net/10033/347315
https://hdl.handle.net/10033/347315
Autor:
Andreas C.W. Jenke, Tobias Eisenberger, Armin Baiker, Isa M. Stehle, Stefan Wirth, Hans Joachim Lipps
Publikováno v:
Human Gene Therapy; Apr2005, Vol. 16 Issue 4, p533-539, 7p
Publikováno v:
Molecular Biology Reports; Jun2004, Vol. 31 Issue 2, p85-90, 6p