Zobrazeno 1 - 10
of 72
pro vyhledávání: '"Irvin Isenberg"'
Publikováno v:
Nucleic Acids Research. 11:3717-3736
We have developed a method of preparing yeast chromatin that facilitates the analysis of nucleoprotein organization. Yeast chromatin, isolated as an insoluble complex, is digested with micrococcal nuclease and fractionated into major insoluble and so
Autor:
Joseph A. D'Anna, Irvin Isenberg
Publikováno v:
Biochemistry. 13:4987-4992
Autor:
Julia Bruce, Janet M. Cardenas, Michael J. Smerdon, Joan M. Miller, Irvin Isenberg, Henry W. Schaup
Publikováno v:
Analytical Biochemistry. 69:531-535
The intrinsic phosphorescence of unstained proteins may be used to locate their position in gels. The method is especially useful for preparative procedures but can be used analytically.
Autor:
Irvin Isenberg, James K.W. Mardian
Publikováno v:
Biochemistry. 17:3825-3833
The inner histones of the yeast, Saccharomyces cerevisiae, have been isolated and identified by their amino acid compositions. H4 appears to be close to its calf and pea counterparts. H2a, H2b, and H3 have diverged. The isolation of the histones was
Autor:
Enoch W. Small, Irvin Isenberg
Publikováno v:
The Journal of Chemical Physics. 66:3347-3351
Moment index displacement can determine the amount of light scatter and zero point displacement in fluorescence time decay data. Two different types of light scatter are considered and shown to have significantly different effects on the analysis of
Autor:
Irvin Isenberg, Steven Spiker
Publikováno v:
Biochemistry. 16:1819-1826
Pea histones H2a, H2b, H3, and H4 have been isolated and their interactions studied by fluorescence anisotropy, light scatter, and circular dichroism. Histones H3 and H4 are almost identical in plants and animals, but plant histones H2a and H2b diffe
Autor:
Irvin Isenberg, Douglas P. Dickinson
Publikováno v:
Microbiology. 128:651-654
Summary: A method is described for the conversion of Schizosaccharomyces pombe cells to spheroplasts using Novozym 234, a commercially available enzyme preparation. Both exponentially growing and stationary phase cells can be quantitatively converted
Autor:
James K.W. Mardian, Irvin Isenberg
Publikováno v:
Analytical Biochemistry. 91:1-12
Methods are described for localizing proteins in unstained gels and accurately excising the regions containing them. A gel elutor, which is all glass with Lucite fittings, is also described. The elutor removes proteins from gels with a high yield and
Publikováno v:
Biochemical and Biophysical Research Communications. 82:129-135
We report that yeast and wheat contain chromosomal proteins belonging to the high mobility group (HMG). This establishes that the HMG's are widely distributed throughout eukaryotes. We further suggest that prokaryotes may also contain HMG's.
Autor:
Joseph A. D'Anna, Irvin Isenberg
Publikováno v:
Biochemistry. 13:2093-2098