Zobrazeno 1 - 10
of 17
pro vyhledávání: '"Iris A. Mastrangelo"'
Autor:
Wei Liu, Paul Hough, Iris A. Mastrangelo, Chengpu Wang, Steven O. Smith, Mahiuddin Ahmed, Takeshi Sato
Publikováno v:
Journal of Molecular Biology. 358:106-119
Soluble oligomers and protofibrils are widely thought to be the toxic forms of the Abeta42 peptide associated with Alzheimer's disease. We have investigated the structure and formation of these assemblies using a new approach in atomic force microsco
Publikováno v:
Biophysical Journal. 66(2):293-298
For inorganic crystals such as calcite (CaCO3), Atomic Force Microscopy (AFM) has provided surface structure at atomic resolution (Ohnesorge and Binnig, 1993). As part of a broad effort to obtain high resolution for an individual protein or protein a
Autor:
Virgil B. Elings, Magdalena Bezanilla, Jay C. Groppe, Paul V. C. Hough, Helen G. Hansma, G. Gurley, Robert L. Sinsheimer, Paul K. Hansma, Iris A. Mastrangelo, Thomas C. Bruice
Publikováno v:
Scanning. 15:296-299
Three advances involving DNA in atomic force microscopy (AFM) are reported here. First a HEPES-Mg buffer has been used that improves the spreading of DNA and provides good DNA coverage with as little as 200-500 picograms per sample. Second, the new "
Publikováno v:
Proceedings of the National Academy of Sciences. 88:5670-5674
Using conventional and scanning transmission electron microscopy, we have examined the physical basis of long-range enhancer effects between distal and proximal elements in a eukaryotic promoter. Specifically, we have studied binding of human transcr
Publikováno v:
Oncogene. 16(2)
The WAF1, Cyclin G and muscle creatine kinase (MCK) genes, all contain multiple copies of the consensus p53-binding element within their regulatory regions. We examined the role of these elements in transactivation of the muscle creatine kinase (MCK)
Publikováno v:
The EMBO journal. 16(11)
The mitochondrial HMG-box transcription factor xl-mtTFA activates bidirectional transcription by binding to a site separating two core promoters in Xenopus laevis mitochondrial DNA (mtDNA). Three independent approaches were used to study the higher o
Autor:
Iris A. Mastrangelo, J. E. Stenger, P. V. C. Hough, Gregory A. Mayr, Yun Wang, P Tegtmeyer, Pin Wang, Michael Reed
Publikováno v:
The EMBO journal. 13(24)
We examined the role of p53 oligomerization in DNA binding and in transactivation. By conventional electron microscopy (EM) and scanning transmission EM, we find that wild-type tetramers contact 18-20 bp at single or tandem 19 bp consensus sequences
Autor:
Nicholas H. Heintz, Nathaniel Heintz, Lisa Dailey, Joseph S. Wall, Paul Held, Iris A. Mastrangelo, Paul V. C. Hough
Publikováno v:
Journal of molecular biology. 232(3)
We show assembly of low and high multimers of HeLa cell nuclear protein, RIP60, at the origin of bidirectional replication (OBR) identified by Burhans, Vassilev, Caddle, Heintz and DePamphilis in Chinese hamster ovary cells. RIP60 binds a 5′-ATT-3
Autor:
Harold H. Smith, Iris A. Mastrangelo
Publikováno v:
Plant Science Letters. 10:171-179
Primary cultured pith cells of Datura innoxia were subjected to a stepwise selection of colonies resistant to the folic acid analog, aminopterin. The first two screenings were done at a concentration of 1 · 10−5 M, followed by 2 · 10−5 M and 4
Autor:
Robert G. Roeder, Michele Sawadogo, James F. Hainfeld, Iris A. Mastrangelo, Joseph S. Wall, Paul V. C. Hough
Publikováno v:
Proceedings of the National Academy of Sciences. 84:4826-4830
The gene-specific transcription initiation factor USF (upstream stimulatory factor) binds at a palindromic sequence that extends from -52 to -63 relative to the start site of the adenovirus type 2 major late promoter; USF enhances in vitro transcript