Výsledky vyhledávání - "Irina Vladimirovna Biryukova"

Pho regulon promoter-mediated transcription of the key pathway gene aroG Fbr improves the performance of an l-phenylalanine-producing Escherichia coli strain

Autor: Irina Sergeevna Tsyrenzhapova, Evgeniya M. Kiseleva, Alexander A. Krylov, Irina Vladimirovna Biryukova, Vera Georgievna Doroshenko, Svetlana M. Kazakova, Vladimir Yu. Ermishev, Sergey V. Mashko

Publikováno v: Applied Microbiology and Biotechnology. 88:1287-1295

DAHP synthase (EC 4.1.2.15) is one of the key enzymes involved in aromatic amino acid biosynthesis in Escherichia coli. An approximately twofold decrease in DAHP synthase activity level was detected in the late growth phase of the l-phenylalanine (Ph

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::a0476db178f09adf76439ef7ffd4c5a9
https://doi.org/10.1007/s00253-010-2794-x

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Conditional Silencing of the Escherichia coli pykF Gene Results from Artificial Convergent Transcription Protected from Rho-Dependent Termination

Autor: Larisa G. Airich, Sergey V. Mashko, Evgeniya M. Kiseleva, N. I. Minaeva, Alexander A. Krylov, Irina Vladimirovna Biryukova

Publikováno v: Microbial Physiology. 18:1-13

PykF is one of two pyruvate kinases in Escherichia coli K-12. λPL was convergently integrated into the chromosome of the MG1655 strain, downstream of pykF, face-to-face with its native promoter. In the presence of λcIts857, efficient pykF ts-silenc

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::aa11e41837b626d7a4cbdccc7ba39f65
https://doi.org/10.1159/000274307

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A new method for the construction of translationally coupled operons in a bacterial chromosome

Autor: Zinaida Polonskaya, A. Yu. Gulevich, S. V. Mashko, Irina Vladimirovna Biryukova, Danila Vadimovich Zimenkov, Alexander A. Krylov, V. Yu. Ermishev, N. I. Minaeva, A. Yu. Skorokhodova

Publikováno v: Molecular Biology. 43:505-514

A new method for the construction of translationally coupled operons in a bacterial chromosome was developed on the basis of the recombineering approach. The method includes the in vitro construction of an artificial operon with an efficiently transl

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::71403e80e7cad3981ce047f66d95788d
https://doi.org/10.1134/s0026893309030194

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Tuning the Expression Level of a Gene Located on a Bacterial Chromosome

Autor: N. I. Minaeva, Vera Georgievna Doroshenko, A. Yu. Gulevich, Irina Vladimirovna Biryukova, S. V. Mashko, A. Yu. Skorokhodova, Danila Vadimovich Zimenkov, Joanna I. Katashkina

Publikováno v: Molecular Biology. 39:719-726

A new method of constructing a set of bacterial cell clones varying in the strength of a promoter upstream of the gene of interest was developed with the use of Escherichia coli MG1655 and lacZ as a reporter. The gist of it lies in constructing a set

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_________::cdea6f56e0f3e65c129ab2722c37c12a
https://doi.org/10.1007/s11008-005-0087-8

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Construction of stably maintained non-mobilizable derivatives of RSF1010 lacking all known elements essential for mobilization

Autor: Joanna I. Katashkina, Irina L. Tokmakova, Lubov I Golubeva, Irina G. Andreeva, Alexandra Yu Skorokhodova, Sergey V. Mashko, Tatiana M. Kuvaeva, Irina Vladimirovna Biryukova

Publikováno v: BMC Biotechnology, Vol 7, Iss 1, p 80 (2007)
BMC Biotechnology

Background RSF1010 is a well-studied broad-host-range plasmid able to be mobilized to different bacteria and plants. RSF1010-derived plasmid vectors are widely used in both basic research and industrial applications. In the latter case, exploiting of

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::45857ff079392a7a1c70beca7138ea81
http://www.biomedcentral.com/1472-6750/7/80

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Dual-In/Out strategy for genes integration into bacterial chromosome: a novel approach to step-by-step construction of plasmid-less marker-less recombinant E. coli strains with predesigned genome structure

Autor: Aleksandra Yu Skorokhodova, Sergey V. Mashko, Irina Vladimirovna Biryukova, Danila Vadimovich Zimenkov, Evgeny R Gak, N. I. Minaeva

Publikováno v: BMC Biotechnology, Vol 8, Iss 1, p 63 (2008)
BMC Biotechnology

Background The development of modern producer strains with metabolically engineered pathways poses special problems that often require manipulating many genes and expressing them individually at different levels or under separate regulatory controls.

Externí odkaz: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c8ad537b9aca0ea3e4164906f140a304
https://doi.org/10.1186/1472-6750-8-63

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